Development of a Dual-Epitope Nanobody-Based Immunosensor with MXenes@CNTs@AuNPs for Ultrasensitive Detection of Rotavirus DOI

Wenjin Hu,

Shixiang Yang,

Xiaolong Wang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(49), P. 19678 - 19686

Published: Nov. 18, 2024

Immunoassays have become essential tools for detecting infectious viruses. However, traditional monoclonal antibody-dependent immunoassays are costly, fragile, and unstable, especially in complex media. To overcome these challenges, we developed cost-effective, robust, high-affinity nanobodies as alternatives to antibodies rapid detection applications. We engineered dual-epitope nanobody (NB) pairs incorporated them into a sandwich immunosensor design detect transmitted rotaviruses rectal swabs wastewater samples. further enhance sensitivity, synthesized an advanced two-dimensional material, MXenes@CNTs@AuNPs, which offers extensive specific surface area that supports the enrichment immobilization of NBs. This integration with catalase-modified magnetic probes facilitates signal generation. Subsequently, our sensor achieved limit 0.0207 pg/mL rotavirus VP6 antigen, significantly outperforming commercial antigen kits sensitivity enhancement 3.77 × 105-fold. The exceptional performance extended specificity, repeatability, stability, accuracy across various sample types, establishing it promising tool detection. research outlines viable strategy creating robust ultrasensitive analytical nanoprobe, thereby addressing critical need efficient reliable viral methods environments.

Language: Английский

Development of a Dual-Epitope Nanobody-Based Immunosensor with MXenes@CNTs@AuNPs for Ultrasensitive Detection of Rotavirus DOI

Wenjin Hu,

Shixiang Yang,

Xiaolong Wang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(49), P. 19678 - 19686

Published: Nov. 18, 2024

Immunoassays have become essential tools for detecting infectious viruses. However, traditional monoclonal antibody-dependent immunoassays are costly, fragile, and unstable, especially in complex media. To overcome these challenges, we developed cost-effective, robust, high-affinity nanobodies as alternatives to antibodies rapid detection applications. We engineered dual-epitope nanobody (NB) pairs incorporated them into a sandwich immunosensor design detect transmitted rotaviruses rectal swabs wastewater samples. further enhance sensitivity, synthesized an advanced two-dimensional material, MXenes@CNTs@AuNPs, which offers extensive specific surface area that supports the enrichment immobilization of NBs. This integration with catalase-modified magnetic probes facilitates signal generation. Subsequently, our sensor achieved limit 0.0207 pg/mL rotavirus VP6 antigen, significantly outperforming commercial antigen kits sensitivity enhancement 3.77 × 105-fold. The exceptional performance extended specificity, repeatability, stability, accuracy across various sample types, establishing it promising tool detection. research outlines viable strategy creating robust ultrasensitive analytical nanoprobe, thereby addressing critical need efficient reliable viral methods environments.

Language: Английский

Citations

1