Effect of host microenvironment and bacterial lifestyles on antimicrobial sensitivity and implications for susceptibility testing DOI Creative Commons

Ramón Garcia-Maset,

Victoria Chu, Ka Y. Yuen

et al.

npj Antimicrobials and Resistance, Journal Year: 2025, Volume and Issue: 3(1)

Published: May 21, 2025

Abstract Bacterial infections remain a major global health issue, with antimicrobial resistance (AMR) worsening the crisis. However, treatment failure can occur even when bacteria show antibiotic susceptibility in diagnostic tests. We explore factors such as phenotypic resilience, bacterial lifestyles biofilms, and differences between laboratory tests real infection sites, highlighting need for improved platforms to better predict outcomes, reviewing emerging technologies aimed at improving testing.

Language: Английский

Advanced biomaterials for targeting mature biofilms in periodontitis therapy DOI
Jie Tao,

Yirong Sun,

Guoliang Wang

et al.

Bioactive Materials, Journal Year: 2025, Volume and Issue: 48, P. 474 - 492

Published: Feb. 27, 2025

Language: Английский

Citations

1
Simulated Media for Mimicking the Human Environment In Vitro DOI Creative Commons
Blake Roberts, Ida Clement Thaarup

Apmis, Journal Year: 2025, Volume and Issue: 133(4)

Published: April 1, 2025

ABSTRACT The phrase ‘All models are wrong but some useful’ spoken by George Box in 1976 is as relevant today ever. Modern research relies heavily on and the use of vitro cornerstone developing novel treatments for various infectious diseases. Simple growth media have been, still are, used when performing involving biofilms pathogens. However, using modern technologies, large discrepancies now being revealed between bacteria grown simple versus those more authentic media. These can lead to significant differences bacterial tolerances, patterns, biofilm formation abilities, etc. Hence, if aim replicate vivo situation a laboratory setting, creation realistic simulated bodily fluids should be prioritised. This paper presents range human from body sites where infections often occur. Bacterial behaviour has been evaluated all these compared medium counterpart. In instances, observed which might important discrepancies, particularly potential treatment efficiency. We hope this may serve inspiration any researcher doing work, attempting mimic reality.

Language: Английский

Citations

0
A pH‐responsive Cascade Nano‐Reactor Elevates ROS Generation by Remodeling Biofilm Microenvironment for Enhanced Antibacterial Treatment DOI

Xinyan Gong,

Linzhu Su,

Shiyu Peng

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract Biofilms are the root of most chronic and persistent infections pose a significant threat to human health. Reactive oxygen species (ROS) generation platforms have been used combat biofilm‐associated infections. However, biofilm microenvironments (BME) such as hypoxia overexpressed antioxidants restrict efficacy ROS‐based therapies. To address problem, this study incorporates calcium peroxide (CaO 2 ) berberine (BBR) into Fe Zn containing bimetal metal–organic frameworks (FZ) construct composite ROS nanogenerator (CBFZ), which is able remodel BME further promotes for enhance eradication. CBFZ degrades release CaO , 3+ 2+, BBR in biofilm, where decomposes O H relieve hypoxia, consumes glutathione (GSH). Subsequently, remodeled boosts production ‐dependent BBR‐mediated photodynamic therapy 2+ ‐based chemodynamic therapy, depleted GSH minimizes scavenging meantime, ultimately maintaining high level biofilm. It demonstrated that can effectively eradicate by killing embedded bacteria dispersing matrix. Moreover, exhibits an outstanding therapeutic effect murine model with subcutaneous infection. Overall, work offers propagable strategy antibiofilm therapy.

Language: Английский

Citations

0
Antibacterial Activity and Mechanism of Litsea cubeba Essential Oil Against Salmonella typhimurium DOI Creative Commons

Cuncai Wang,

Xiying Chen,

Mingjie Liu

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(9), P. 1343 - 1343

Published: April 29, 2025

Litsea cubeba essential oil (LCEO) has been reported as an antibacterial agent, but its effects against Salmonella typhimurium (S.Tm) and the underlying mechanisms remain unclear. The efficacy of LCEO was assessed utilizing both microdilution growth curve methodologies, chemical composition thoroughly analyzed. Morphological alterations in cells were observed through scanning electron microscopy (SEM), while cellular permeability gauged based on variations nucleic acid protein contents. impact ATPase activity anti-biofilm formation using colorimetric methods. results indicated that MIC MBC S.Tm 0.4 mg/mL 0.8 mg/mL, respectively. SEM PI staining revealed disrupted bacterial cell integrity. Compared to those control group, treatment with significantly elevated levels extracellular acids proteins (p < 0.05). Furthermore, at MIC, led a 77.9% reduction AKP content, decreased intracellular Na+K+-ATPase Ca2+Mg2+-ATPase activities by 79.9% 54.6%, Additionally, markedly inhibited biofilm formation, enhanced surface hydrophobicity, diminished swimming motility S.Tm. Overall, exhibited promising properties, indicating potential effective inhibitor

Language: Английский

Citations

0
Effect of host microenvironment and bacterial lifestyles on antimicrobial sensitivity and implications for susceptibility testing DOI Creative Commons

Ramón Garcia-Maset,

Victoria Chu, Ka Y. Yuen

et al.

npj Antimicrobials and Resistance, Journal Year: 2025, Volume and Issue: 3(1)

Published: May 21, 2025

Abstract Bacterial infections remain a major global health issue, with antimicrobial resistance (AMR) worsening the crisis. However, treatment failure can occur even when bacteria show antibiotic susceptibility in diagnostic tests. We explore factors such as phenotypic resilience, bacterial lifestyles biofilms, and differences between laboratory tests real infection sites, highlighting need for improved platforms to better predict outcomes, reviewing emerging technologies aimed at improving testing.

Language: Английский

Citations

0