Rapid and Accurate Diagnosis of Urinary Tract Infections Using Targeted Next-Generation Sequencing: A Multicenter Comparative Study with Metagenomic Sequencing and Traditional Culture Methods

Journal of Infection, Journal Year: 2025, Volume and Issue: 90(4), P. 106459 - 106459

Published: March 7, 2025

BACKGROUNDUrinary tract infections (UTIs) rank among the most prevalent bacterial globally. Traditional urine culture methods have significant limitations in detection time and sensitivity, prompting need to evaluate targeted next-generation sequencing (tNGS) as a potential diagnostic tool.METHODSThe study included discovery cohort of 400 suspected UTI patients (202 analyzed) validation 200 (110 analyzed). The assessed time, concordance rates, ability identify polymicrobial infections, antibiotic resistance genes (ARGs). Both clear turbid samples were evaluated across different clinical settings.RESULTSIn cohort, tNGS demonstrated 96.5% with culture-positive samples, while showing superior specificity culture-negative specimens (53.1% vs 28.1% for mNGS). Detection (12.89h) was notably shorter than mNGS (17.38h) traditional (61.48h). exhibited remarkable capability identifying (55.4% samples), significantly outperforming both (27.7%) methods, which failed detect any co-infections. method showed particular strength detecting fastidious organisms like Ureaplasma parvum fungal species such Candida tropicalis. For prediction, detected more ARGs (52.67% 41.22% mNGS) achieved 100% sensitivity vancomycin methicillin Gram-positive pathogens. confirmed tNGS's robust performance, maintaining high rates (90.00%) (55.00%), demonstrating consistent reliability settingsCONCLUSIONStNGS demonstrates advantages rapid accurate diagnosis, particularly analyzing genes. It shows promise an effective complementary tool diagnostics.

Language: Английский

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Decoding Urinary Tract Infection Trends: A 5-Year Snapshot from Central Portugal

Clinics and Practice, Journal Year: 2025, Volume and Issue: 15(1), P. 14 - 14

Published: Jan. 6, 2025

This study analyzes urinary tract infections (UTIs) in a hospital Central Portugal over five-year period, focusing on bacterial prevalence, patient demographics, and antibiotic resistance patterns. investigation aims to provide insights that can guide improved infection control treatment strategies. A total of 6161 positive urine cultures collected five years were examined, with particular emphasis 2019 due peak rates. The analysis explored demographic factors such as sex clinical service origin, resistance. Special attention was given hospitalized patients, especially those undergoing invasive procedures, their increased vulnerability infection. found UTIs more prevalent female reflecting anatomical susceptibilities. Hospitalized individuals, particularly requiring at greater risk. predominant bacteria Escherichia coli, Klebsiella pneumoniae, Enterococcus faecalis, differences prevalence by origin. Resistance Imipenem E. coli increased, raising concerns about last-resort treatments. However, other antibiotics declined, suggesting improvements recent stewardship measures. During the COVID-19 pandemic, overall consumption decreased changes practices. findings highlight importance strict control, targeted prevention measures, rational use combat Ongoing surveillance personalized approaches are essential improve UTI management outcomes.

Language: Английский

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A Novel Silver-Ruthenium-Based Antimicrobial Kills Gram-Negative Bacteria Through Oxidative Stress-Induced Macromolecular Damage

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 4, 2025

ABSTRACT Amplified by the decline in antibiotic discovery, rise of resistance has become a significant global challenge infectious disease control. Extraintestinal Escherichia coli (ExPEC), known to be most common instigators urinary tract infections (UTIs), represent such threat. Novel strategies for more efficient treatments are therefore desperately needed. These include silver nanoparticles, which have been used as antimicrobial surface-coatings on catheters eliminate biofilm-forming uropathogens and reduce risk nosocomial infections. AGXX® is promising coating that presumably kills bacteria through generation reactive oxygen species (ROS) but potent than silver. However, neither AGXX®’s mode action fully understood, nor its effects Gram-negative or bacterial response defense mechanisms towards studied detail. Here, we report bactericidal primarily based ROS formation, supplementation media with scavenger completely abolished AGXX®-induced killing. We further show impairs integrity cell envelope causes substantial protein aggregation DNA damage already at sublethal concentrations. ExPEC strains appear resistant proteotoxic compared non-pathogenic E. coli, indicating improved capabilities uropathogen. Global transcriptomic studies AGXX®-stressed revealed strong oxidative stress response, perturbations metal homeostasis, well activation heat shock responses. Finally, present evidence counter production chaperone polyphosphate.

Language: Английский

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Bacteriological Profile and Antibiotic Sensitivity Pattern of Urinary Tract Infection

East West Medical College journal., Journal Year: 2025, Volume and Issue: 13(1), P. 23 - 27

Published: Jan. 8, 2025

Introduction:Urinary tract infection (UTI) is a common that may occur in any part of the urinary system - kidneys, ureters, bladder and urethra. UTIs are caused by wide range pathogens, recurrence rates high. Antibiotic resistance to microorganism burning issue all over world due inappropriate antibiotic use. leads morbidity, mortality overall, economic burden. Therefore, surveillance antimicrobial UTI essential see its impact on population health local treatment practices. Objective: This study was conducted identify bacterial agents infections determine antibiogram patterns. Methods: A cross-sectional at outpatient service. The included patients who presented with symptoms frequency, urgency, hematuria, dysuria, suprapubic discomfort. Those clinical manifestations but antibiotics or completed within past five days were excluded. Results: prevalence high among females (88.67%) than males (13.33%). Among 30 samples, pathogens implicated Escherichia coli (E. coli) 24 (80%), Klebsiella 3. Organisms showed higher sensitivity toward Meropenem Imipenem (100%) followed Amikacin Nitrofurantoin (96.6%). Isolates organisms levels betalactamaes ampicillin (60%) cephalosporines groups. According profiles, most effective oral (96%) Ciprofloxacin (90%). Conclusion:This findings emphasize significance patterns infection. More studies can be done create national guidelines physicians should use rational adequate dose avoid emergence resistance. EWMCJ Vol. 13, No. 1, January 2025: 23-27

Language: Английский

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The natural immune molecules urinary Tamm–Horsfall protein and pentraxin 3 as predictors for recurrent urinary tract infection severity: a single-center self-control study

Renal Failure, Journal Year: 2025, Volume and Issue: 47(1)

Published: Jan. 8, 2025

Objective The innate immune defense plays a pivotal role in protecting the urinary tract from uropathogenic invasion and maintaining homeostasis. Dysregulation of system can result recurrent infections (RUTI) due to heightened susceptibility uropathogens. Despite this, predicting risk recurrence degree compromise patients who have had one infection remains challenging. Also identifying which are more susceptible developing pyelonephritis rather than local disease cystitis is imperfect, although delayed diagnosis UTI good indicator for pyelonephritis. This study aims assess potential Tamm-Horsfall protein (THP) Pentraxin 3 (PTX3) as predictors RUTI symptom severity recurrence, while also evaluating efficacy Chinese herbal formulation Tailin Formula (TLF) clinical therapeutic intervention RUTI.

Language: Английский

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Advances in Enterococcal Urinary Tract Infection Research

Advances in Clinical Medicine, Journal Year: 2025, Volume and Issue: 15(01), P. 878 - 885

Published: Jan. 1, 2025

Language: Английский

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Bioactive Compounds as Alternative Approaches for Preventing Urinary Tract Infections in the Era of Antibiotic Resistance

Antibiotics, Journal Year: 2025, Volume and Issue: 14(2), P. 144 - 144

Published: Feb. 1, 2025

Urinary tract infections (UTIs) are among the most common bacterial worldwide. They occur in urinary system when a microorganism, commonly present on perineal skin or rectum, reaches bladder through urethra, and adheres to luminal surface of uroepithelial cells, forming biofilms. The treatment UTIs includes antibiotics, but their indiscriminate use has favored development multidrug-resistant bacteria strains, which represent serious challenge today’s microbiology. pathogenesis infection antibiotic resistance synergistically contribute hindering eradication disease while favoring establishment persistent infections. repeated requirement for limited therapeutic options have further contributed increase occurrence potential relapses by failure. To limit antimicrobial broaden choice non-antibiotic preventive approaches, this review reports studies focused bacteriostatic/bactericidal activity, inhibition adhesion quorum sensing, restoration integrity immune response molecules, vitamins, compounds obtained from plants. date, different supplementations recommended European Association Urology management as an alternative approach treatment, variety bioactive under investigation, mostly at level vitro preclinical studies. Although evidence is promising, they far being included clinical practice UTIs.

Language: Английский

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Evaluating Quality of Life of Prostate Cancer Patients After Radical Prostatectomy

Cureus, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Background Prostate cancer is the second most commonly diagnosed globally and has a significant impact on patients' quality of life (QoL). The treatment prostate cancer, particularly through radical prostatectomy, offers curative benefits but often results in adverse effects such as urinary incontinence erectile dysfunction. These issues can persist long after treatment, affecting both physical mental well-being. Beyond these complications, patients may also experience emotional distress, anxiety, social implications, changes relationships self-perception. As such, evaluating QoL post-treatment essential understanding comprehensive care. Methodology This study involved 110 who underwent prostatectomy. was assessed using Short-Form Health Survey Questionnaire (SF-36) at following three points: before six 12 months post-treatment. evaluation focused physical, emotional, aspects health. Patients with psychiatric disorders or those unable to understand Greek were excluded from study. Results participants' average age ranged 67 82 years (mean = 74.2 years). A improvement functioning observed between first assessments. Improvements seen functionality, well-being, general health, not statistically significant. By third assessment (12 post-surgery), improvements noted vitality, though other changes, including role functioning, Conclusions Radical prostatectomy effective for control persistent dysfunction incontinence. While there are certain areas vitality managing full spectrum psychosocial impacts requires holistic approach. Incorporating psychological support sexual rehabilitation into plans enhance patients. integration patient-reported outcomes clinical care crucial ensuring treatment.

Language: Английский

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Fluorescent sensor array for rapid bacterial identification using antimicrobial peptide-functionalized gold nanoclusters and machine learning

Talanta, Journal Year: 2025, Volume and Issue: 291, P. 127883 - 127883

Published: March 3, 2025

Language: Английский

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Glucose alters the evolutionary response to gentamicin in uropathogenic Escherichia coli

Microbiology, Journal Year: 2025, Volume and Issue: 171(3)

Published: March 28, 2025

Urinary tract infections (UTI) are a major health and economic concern. Uropathogenic Escherichia coli (UPEC) the leading cause of UTI, antibiotic-resistant UPEC increasingly common. The microenvironment urinary is metabolically distinct, there growing interest in understanding extent to which metabolism may influence infection response antibiotics, how this varies between individuals. Diabetes, characterized part by glycosuria, known risk factor for UTI associated with more severe infections. role that glucose plays driving evolution remains unclear. Through experimental single isolate, we identified mutations RNA polymerase sigma rpoS long-term exposure. We found presence antibiotic gentamicin resulted genes including trkH , encodes potassium ion uptake system previously linked aminoglycoside resistance, autotransporter hyxB . Strikingly, these were not present populations exposed combination both gentamicin. This suggests survival mutants gentamicin, providing new avenues treatment UPEC-mediated high-risk

Language: Английский

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