Developmental Dynamics,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 21, 2025
Abstract
Background
Skeletal
muscle
stem
cells
(MuSCs)
played
an
important
role
in
maintaining
the
proper
function
of
tissues.
In
adults,
they
normally
remained
a
quiescent
state
and
activated
upon
stimulation
to
undergo
self‐renewal
or
myogenic
differentiation.
This
process
was
complexly
regulated
by
cytokines,
molecular
mechanisms
that
promoted
MuSCs
activation
largely
unknown.
Results
Here,
we
analyzed
transcriptome
data
from
different
stimuli
using
weighted
gene
co‐expression
network
analysis
(WGCNA)
identified
key
long
non‐coding
RNA
SNHG1
(lncSNHG1),
which
promotes
transition
MuSCs.
Overexpression
lncSNHG1
able
promote
proliferation
differentiation
MuSCs,
whereas
knockdown
resulted
opposite
results.
Mechanistically,
disruption
Wnt/β‐catenin
pathway
blocked
quiescence
exit
induced
lncSNHG1.
Conclusions
We
conclude
is
factor
cell
through
pathway.
Stroke and Vascular Neurology,
Journal Year:
2024,
Volume and Issue:
unknown, P. svn - 002883
Published: Jan. 29, 2024
The
use
of
biologics
in
various
diseases
has
dramatically
increased
recent
years.
Stroke,
a
cerebrovascular
disease,
is
the
second
most
common
cause
death,
and
leading
disability
with
high
morbidity
worldwide.
For
applied
treatment
acute
ischaemic
stroke,
alteplase
only
thrombolytic
agent.
Meanwhile,
current
clinical
trials
show
that
two
recombinant
proteins,
tenecteplase
non-immunogenic
staphylokinase,
are
promising
as
new
agents
for
stroke
therapy.
In
addition,
stem
cell-based
therapy,
which
uses
cells
or
organoids
treatment,
shown
results
preclinical
early
studies.
These
strategies
mainly
rely
on
unique
properties
undifferentiated
to
facilitate
tissue
repair
regeneration.
However,
there
still
considerable
journey
ahead
before
these
approaches
become
routine
use.
This
includes
optimising
cell
delivery
methods,
determining
ideal
type
dosage,
addressing
long-term
safety
concerns.
review
introduces
proteins
thrombolysis
therapy
highlights
promise
challenges
cerebral
Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: July 6, 2024
Abstract
Quiescence,
a
hallmark
of
adult
neural
stem
cells
(NSCs),
is
required
for
maintaining
the
NSC
pool
to
support
life-long
continuous
neurogenesis
in
dentate
gyrus
(DG).
Whether
long-lasting
epigenetic
modifications
maintain
quiescence
over
long
term
DG
not
well-understood.
Here
we
show
that
mice
with
haploinsufficiency
Setd1a
,
schizophrenia
risk
gene
encoding
histone
H3K4
methyltransferase,
develop
an
enlarged
more
granule
after
young
adulthood.
Deletion
specifically
quiescent
NSCs
promotes
their
activation
and
neurogenesis,
which
countered
by
inhibition
demethylase
LSD1.
Mechanistically,
RNA-sequencing
CUT
&
RUN
analyses
cultured
reveal
deletion-induced
transcriptional
changes
many
targets,
among
down-regulation
Bhlhe40
vivo.
Together,
our
study
reveals
Setd1a-dependent
mechanism
sustains
DG.
Cell Regeneration,
Journal Year:
2024,
Volume and Issue:
13(1)
Published: Sept. 30, 2024
Abstract
Tissue
stem
cells
(TSCs),
which
reside
in
specialized
tissues,
constitute
the
major
cell
sources
for
tissue
homeostasis
and
regeneration,
contribution
of
transcriptional
or
epigenetic
regulation
distinct
biological
processes
TSCs
has
been
discussed
past
few
decades.
Meanwhile,
ATP-dependent
chromatin
remodelers
use
energy
from
ATP
hydrolysis
to
remodel
nucleosomes,
thereby
affecting
dynamics
gene
expression
programs
each
type.
However,
role
fate
determination
is
less
well
understood.
In
this
review,
we
systematically
discuss
recent
advances
control
by
hematopoietic
cells,
intestinal
epithelial
neural
skin
their
highlight
importance
essential
homeostasis,
development,
regeneration.
Moreover,
exploration
molecular
cellular
mechanisms
crucial
advancing
our
understanding
maintenance
discovery
novel
therapeutic
targets.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 6, 2025
Abstract
Obesity
is
linked
to
limited
adipose
tissue
(AT)
remodeling
capacity,
leading
hypertrophic
adipocytes,
senescence,
and
inflammation.
We
used
a
mouse
model
expressing
mTert
(p21
+/Tert
)
from
the
Cdkn1a
locus
investigate
role
of
mTERT
in
obesity-induced
metabolic
disorders.
Conditional
expression
reduces
disorders
associated
with
obesity.
In
AT,
this
accompanied
by
decrease
number
senescent
p21-positive
cells,
very
short
telomeres,
oxidative
DNA
damage.
Single
nucleus
RNA-seq
data
reveal
TERT
attenuates
senescence
induced
HFD
particular
stem
progenitor
cells
(ASPC).
show
that
ASPC
expansion
differentiation
are
promoted
p21
obese
mice,
thereby
reducing
further
report
remodels
landscape
macrophages
AT
mice.
Strikingly,
inactivation
catalytic
activity
+/TertCi
mice
suppresses
promotion
adipocyte
formation,
but
neither
affects
attenuation
nor
macrophage
remodeling.
These
results
highlight
mTERT’s
canonical
non-canonical
functions
obesity-associated
thus
appears
as
potential
therapeutic
option
for
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 14, 2025
ABSTRACT
Colonic
stem
cells
have
a
key
role
in
the
continuous
regeneration
of
healthy
intestinal
epithelium.
Despite
considerable
advances
single-cell
omics
technologies,
transcriptional
heterogeneity
rare
cell
types
such
as
colonic
cells,
well
their
functional
states
and
niche-specific
behaviors,
remain
poorly
characterised.
In
this
study,
we
leverage
meta-analysis
scRNA-seq
spatial
transcriptomic
datasets
to
comprehensively
map
cells.
We
identify
multiple,
previously
underappreciated
states,
including
distinct
quiescent
subtypes
marked
by
CDKN1A
(P21),
CDKN1B
(P27),
CDKN1C
(P57),
proliferative
populations
defined
MKI67
(Ki67)
LRIG1
,
lineage-committed
intermediate
subpopulation
expressing
MUC2
.
Strikingly,
find
that
these
can
be
robustly
identified
solely
extracellular
matrix
(ECM)
gene
expression
signatures,
revealing
ECM
composition
critical
determinant
identity.
Notably,
LAMA1
is
highly
specific
P57+
population,
linking
laminin-mediated
microenvironments
active
maintenance
deep
quiescence,
consistent
with
our
recent
findings
associating
survival.
By
applying
delineate
discrete
“micro-niches”
tissue
provide
evidence
analogous
persist
colorectal
cancer
(CRC)
environment.
Extending
approach
an
unrelated
tissue,
pancreas,
detect
parallel
subtypes,
illustrating
broader
applicability
ECM-based
signatures.
Taken
together,
redefine
concept
colon,
establish
ECM-driven
signatures
powerful
tool
for
characterizing
offer
new
perspectives
on
niche-dependent
regulation
both
cancerous
populations.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 9, 2025
Abstract
The
functional
capacity
of
highly
proliferative
cell
populations
changes
with
age.
Here,
we
report
that
the
canine
lung
mesenchymal
stromal
cells
(LMSCs)
declines
increasing
age
donor.
However,
other
such
as
reduced
autophagy,
migration/wound
healing,
increased
production
reactive
oxygen
species,
and
senescence
are
not
significantly
altered
Furthermore,
transcriptomic
profiling
suggests
minimal
age-related
changes.
These
data
suggest
LMSCs
isolated
from
aging
donors
may
be
associated
reversible
cycle
arrest
(quiescence),
rather
than
irreversible
(senescence).
Similar
findings
have
been
reported
in
systems,
including
neural
muscle
stem
low
turnover-rate
tissues.
