A Dual-Channel Fluorescence Probe for Early Diagnosis and Treatment Monitoring of Acute Kidney Injury by Detecting HOCl and Cys with Different Fluorescence Signals

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

The pathogenesis of acute kidney injury (AKI) is a multifaceted process involving various mechanisms, with oxidative stress playing crucial role in its development. Hypochlorite (HOCl) and cysteine (Cys) are indicators AKI pathophysiology, directly reflecting the degree disease severity. However, their exact mechanism action pathophysiology remains unknown. Herein, we developed dual-channel fluorescent probe, MB-NAP, which allowed for simultaneous detection HOCl Cys. probe exhibited distinct fluorescence responses green channel (λex = 450 nm, λem 560 nm) red 610 690 nm), without any spectral crosstalk, allowing accurate measurement both analytes. We successfully applied MB-NAP to monitor levels Cys cellular vivo models AKI, revealing significant increase concentrations compared normal models. Furthermore, was demonstrated exhibit outstanding capabilities drug screening by effectively real-time monitoring This study not only provides more sensitive reliable method/tool tracking AKI-related pathological processes but also offers potential breakthrough early diagnosis identification therapeutic agents aimed at mitigating stress-induced damage AKI.

Language: Английский

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Biomimetic Sealing of Cisplatin by Cancer Cell Membranes to Achieve Nucleophile Resistance and Tumor Targeting for Improved Cancer Therapy

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 14, 2025

Platinum-based anticancer drugs (PBCs), particularly cisplatin, play a key role in over 70% of cancer treatment protocols. PBCs suffer from their strong affinity with numerous nucleophiles present the body, leading to significant systematic toxicity and rapid drug inactivation. The cell membrane's selective energy-dependent transport properties, inherent its unique biological structure, offer strategic opportunity for employing membranes (CMs) development PBC delivery systems that repel nucleophiles. To prove this idea, we harness CMs develop dual-package approach sealing cisplatin nanoformulation is both nucleophile-resistant tumor-targeted without need synthetic materials. process begins by conjugating CMs, creating positively charged nanoparticles. These isolated nanoparticles are then recomplexed CMs. Our strategy, which tightly seals within ensures safely sequestered reactive molecules body while simultaneously guiding it specifically homologous tumors. resulting demonstrates immune evasion prolonged circulation time due native-like identity conferred biomimetic prevented transmembrane attack nucleophiles, including not only macromolecular proteins but also small-molecule compounds such as glutathione, thereby ensuring high level cytotoxicity when challenged these It displays precise targeting at tumors, sustained release, achieves tumor suppression. features together adumbrate nanoformulation's potential revolutionary tool therapy. Given prevalence metal ion-based common susceptibility nucleophile-associated issues, strategy presented study may widely applicable solution developing metal-ion-based medications.

Language: Английский

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RGD hydrogel-loaded ADSC extracellular vesicles mitigate uranium-induced renal injury via TLR4/NF-κB pathway inhibition

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 17, 2025

Uranium-induced kidney damage represents a major health concern due to its toxic effects, including mitochondrial dysfunction and inflammation. Mitochondrial DNA (mtDNA)-mediated pyroptosis is critical pathway in the pathogenesis of renal injury. The toll-like receptor 4 / nuclear factor-kappa B (TLR4/NF-κB) signaling plays pivotal role this process. Recent studies have shown that extracellular vesicles derived from adipose-derived stem cells (ADSCs-EVs) possess therapeutic potential their anti-inflammatory regenerative properties. Incorporating ADSCs-EVs into arginine-glycine-aspartate (RGD), hydrogels may enhance stability efficacy vivo. This study aims explore molecular mechanism by which RGD hydrogel-loaded modulate mtDNA-mediated suppressing TLR4/NF-κB alleviate uranium-induced Repairing was found mitigate mtDNA leakage, thereby inhibiting pyroptosis. alleviated cell pathway. In vivo animal experiments confirmed enhanced body improved against Our findings reveal effectively inhibit pathway, preventing alleviating damage. elucidation provides novel strategy for utilizing treating

Language: Английский

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Therapeutic Approaches with Iron Oxide Nanoparticles to Induce Ferroptosis and Overcome Radioresistance in Cancers

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(3), P. 325 - 325

Published: Feb. 26, 2025

The emergence of nanotechnology in medicine, particularly using iron oxide nanoparticles (IONPs), may impact cancer treatment strategies. IONPs exhibit unique properties, such as superparamagnetism, biocompatibility, and ease surface modification, making them ideal candidates for imaging, therapeutic interventions. Their application targeted drug delivery, especially with traditional chemotherapeutic agents like cisplatin, has shown potential overcoming limitations low bioavailability systemic toxicity chemotherapies. Moreover, IONPs, by releasing ions, can induce ferroptosis, a form iron-dependent cell death, which offers promising pathway to reverse radio- chemoresistance therapy. In particular, demonstrate significant radiosensitisers, enhancing the effects radiotherapy promoting reactive oxygen species (ROS) generation, lipid peroxidation, modulating tumour microenvironment stimulate antitumour immune responses. This review explores multifunctional roles radiosensitisation through ferroptosis induction, highlighting their promise advancing head neck cancers. Additional research is crucial fully addressing clinical settings, offering novel approach personalised treatment.

Language: Английский

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Kidney-Targeted Near-Infrared Fluorescence Probe Reveals That SO₂ Is a Biomarker for Cisplatin-Induced Acute Kidney Injury

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(34), P. 12948 - 12955

Published: Aug. 17, 2023

With the widespread use of drugs, drug-induced acute kidney injury (AKI) has become an increasingly serious health concern worldwide. Currently, early diagnosis AKI remains challenging because lack effective biomarkers and noninvasive imaging tools. SO2 plays important physiological roles in living systems is antioxidant for maintaining redox homeostasis. However, relationship between (in water as SO32-/HSO3-) largely unknown. Herein, we report highly sensitive near-infrared fluorescence probe DSMN, which first time reveals AKI. The responds to SO32-/HSO3- selectively rapidly (within seconds) shows a significant turn-on at 710 nm with large Stokes shift (125 nm). these properties, was successfully applied detect cells mice. Importantly, can target kidneys, allowing detection changes concentration kidneys. Based on this, DSMN used cisplatin-induced revealed increase levels. results indicate that new biomarker powerful tool studying diagnosing

Language: Английский

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