Umbelliferone as an effective component of Rhodiola for protecting the cerebral microvascular endothelial barrier in cSVD DOI Creative Commons
Li Tang,

Hongfa Cheng,

Qiuyue Yang

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 17, 2025

Rhodiola is a common Chinese herb in the treatment of cerebral small vessel disease (cSVD). Umbelliferone, one effective components Rhodiola, can protect endothelial barrier. But its mechanisms are still unclear. Therefore, this study aimed to explore umbelliferone an component protecting microvascular barrier cSVD. Firstly, ETCM, SwissTargetPrediction and literatures were used screen targets Rhodiola. GeneCards was obtain STRING Cytoscape utilized for building PPI C-T network. Metascape construct GO KEGG enrichment analysis. Then, molecular docking employed evaluate binding ability compounds their respective target molecules. Ultimately, cell damage caused by OGD protective impact umbelliferone, bioactive constituent on Endothelial leakage migration assays assess permeability cells. IF WB techniques ascertain expression tight junction protein. The major proteins related pathways validated WB. Six 106 potential identified 1885 cSVD obtained. Nine key selected. analysis suggested that effects associated with PI3K-Akt, Ras, Rap1 MAPK signal pathways. Molecular results showed good between 28 pairs compounds. Umbelliferone improve cells damaged through MMP9, MMP2, CCND1, PTGS2 signaling Our systematically clarified treating network pharmacology docking, characterized multi-component, multi-target multi-pathway effects. This finding vitro tests, which demonstrated brain It provided valuable ideas references additional research.

Language: Английский

The immunology of stroke and dementia DOI
Costantino Iadecola, Josef Anrather

Immunity, Journal Year: 2025, Volume and Issue: 58(1), P. 18 - 39

Published: Jan. 1, 2025

Language: Английский

Citations

0
Integration of MRI radiomics and clinical data for preoperative prediction of vascular invasion in breast cancer: A deep learning approach DOI
Guoyuan Pan, Zejun Pan,

Wubiao Chen

et al.

Magnetic Resonance Imaging, Journal Year: 2025, Volume and Issue: unknown, P. 110339 - 110339

Published: Jan. 1, 2025

Language: Английский

Citations

0
Association of cortical macrostructural and microstructural changes with cognitive performance and gene expression in subcortical ischemic vascular disease patients with cognitive impairment DOI Creative Commons

Jing Huang,

Runtian Cheng,

Xiaoshuang Liu

et al.

Brain Research Bulletin, Journal Year: 2025, Volume and Issue: 222, P. 111239 - 111239

Published: Feb. 3, 2025

Previous researches have demonstrated that patients with subcortical ischemic vascular disease (SIVD) exhibited brain structure abnormalities. However, the cortical macrostructural and microstructural characteristics their relationship cognitive scores gene expression in SIVD remain largely unknown. This study collected 3D-T1 diffusion tensor imaging data from 30 impairment (SIVD-CI) 32 normal controls. The between-group comparative analyses of thickness, area, volume, local gyrification index (LGI), mean diffusivity (MD) were conducted a general linear model. Moreover, associations between significant neuroimaging values Allen Human Brain Atlas database evaluated using partial least squares regression correlation analysis. SIVD-CI showed decreases thicknesses across 18 regions, volumes three LGI five as well increases MD regions (P < 0.05). significantly reduced right insula, left superior temporal gyrus, central anterior caudal cingulate cortex, positively correlated different Furthermore, abnormal structural indicators found to be related nine risk genes (VCAN, APOE, EFEMP1, SALL1, BCAN, KCNK2, EPN2, DENND1B XKR6) specific damage may decline patients.

Language: Английский

Citations

0
Genomics of diffusion-imaging integrating GWAS, exome data and single-cell sequencing unravels lifespan determinants of cerebral small vessel disease DOI Creative Commons
Stéphanie Debette, Yasaman Saba, Hideaki Suzuki

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 14, 2025

Abstract Peak width of skeletonized mean diffusivity (PSMD) is an emerging automated diffusion imaging marker showing clinically relevant changes in cerebral small vessel disease (cSVD), a leading cause stroke and dementia with no mechanism-based treatment. We conducted genome-wide association study PSMD 58,403 participants from 24 population-based cohorts (89% European, 10% East-Asian, 1% African-American), identifying 31 independent common variant associations. Additionally, whole-exome sequencing analysis 32,957 yielded associations single burden rare coding variants four novel genes. Mendelian randomization supported causal higher blood pressure larger values, increased risk stroke, especially intracerebral hemorrhage. Strikingly, genetic susceptibility to white matter hyperintensities, established MRI-marker cSVD, was associated early childhood older age, prominent lifespan effects for VCAN SMG6. Leveraging unique brain single-cell resources we showed temporal the cell-type specificity these genes developing overall enrichment loci expressed fetal endothelial cells. Finally, through extensive integration multi-omics resources, provide precious leads gene prioritization accelerate drug discovery cSVD.

Language: Английский

Citations

0
Umbelliferone as an effective component of Rhodiola for protecting the cerebral microvascular endothelial barrier in cSVD DOI Creative Commons
Li Tang,

Hongfa Cheng,

Qiuyue Yang

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 17, 2025

Rhodiola is a common Chinese herb in the treatment of cerebral small vessel disease (cSVD). Umbelliferone, one effective components Rhodiola, can protect endothelial barrier. But its mechanisms are still unclear. Therefore, this study aimed to explore umbelliferone an component protecting microvascular barrier cSVD. Firstly, ETCM, SwissTargetPrediction and literatures were used screen targets Rhodiola. GeneCards was obtain STRING Cytoscape utilized for building PPI C-T network. Metascape construct GO KEGG enrichment analysis. Then, molecular docking employed evaluate binding ability compounds their respective target molecules. Ultimately, cell damage caused by OGD protective impact umbelliferone, bioactive constituent on Endothelial leakage migration assays assess permeability cells. IF WB techniques ascertain expression tight junction protein. The major proteins related pathways validated WB. Six 106 potential identified 1885 cSVD obtained. Nine key selected. analysis suggested that effects associated with PI3K-Akt, Ras, Rap1 MAPK signal pathways. Molecular results showed good between 28 pairs compounds. Umbelliferone improve cells damaged through MMP9, MMP2, CCND1, PTGS2 signaling Our systematically clarified treating network pharmacology docking, characterized multi-component, multi-target multi-pathway effects. This finding vitro tests, which demonstrated brain It provided valuable ideas references additional research.

Language: Английский

Citations

0