Multi‐Functional Carbon Dots for Visualizing and Modulating ROS‐Induced Mitophagy in Living Cells DOI
Hao Li,

Jiaqing Guo,

Aikun Liu

et al.

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 33(17)

Published: Jan. 29, 2023

Abstract Reactive oxygen species (ROS)‐induced mitophagy is associated with a variety of diseases. Therefore, visualizing and modulating the process ROS‐induced essential for understanding role in cellular homeostasis, physiological processes, pathogenesis. Herein, using fluorescence lifetime imaging microscopy (FLIM), first time complete dynamic PINK1/Parkin pathway‐mediated described. Induced by photo‐controlled release ROS, nitrogen‐doped multi‐functional carbon nanozymes (ENZ‐NCDs) used as probe to visualize quantitatively study mitophagy. The successful preparation ENZ‐NCDs provides potentially powerful tool new strategy real‐time monitoring future quantification mitochondrial damage caused ROS mitophagy‐related

Language: Английский

PINK1/PARKIN signalling in neurodegeneration and neuroinflammation DOI Creative Commons
Peter M. J. Quinn, Paula I. Moreira, António Francisco Ambrósio

et al.

Acta Neuropathologica Communications, Journal Year: 2020, Volume and Issue: 8(1)

Published: Nov. 9, 2020

Abstract Mutations in the PTEN-induced kinase 1 (PINK1) and Parkin RBR E3 ubiquitin-protein ligase (PARKIN) genes are associated with familial forms of Parkinson’s disease (PD). PINK1, a protein kinase, PARKIN, an ubiquitin ligase, control specific elimination dysfunctional or superfluous mitochondria, thus fine-tuning mitochondrial network preserving energy metabolism. PINK1 regulates PARKIN translocation impaired mitochondria drives their removal via selective autophagy, process known as mitophagy. As knowledge obtained using different transgenic animal models is being gathered, growing evidence supports contribution mitophagy impairment to several human pathologies, including PD Alzheimer’s diseases (AD). Therefore, therapeutic interventions aiming modulate PINK1/PARKIN signalling might have potential treat these diseases. In this review, we will start by discussing how interplay helps mediate physiology. We continue debating role dysfunction disorders such amyotrophic lateral sclerosis, Alzheimer’s, Huntington’s diseases, well eye age-related macular degeneration glaucoma, causative factors leading PINK1/PARKIN-mediated neurodegeneration neuroinflammation. Finally, discuss gene augmentation possibilities particular focus on AD, glaucoma.

Language: Английский

Citations

323
Pleiotropic Mitochondria: The Influence of Mitochondria on Neuronal Development and Disease DOI Creative Commons
Vidhya Rangaraju, Tommy L. Lewis, Yusuke Hirabayashi

et al.

Journal of Neuroscience, Journal Year: 2019, Volume and Issue: 39(42), P. 8200 - 8208

Published: Oct. 16, 2019

Mitochondria play many important biological roles, including ATP production, lipid biogenesis, ROS regulation, and calcium clearance. In neurons, the mitochondrion is an essential organelle for metabolism homeostasis. Moreover, mitochondria are extremely dynamic able to divide, fuse, move along microtubule tracks ensure their distribution neuronal periphery. Mitochondrial dysfunction altered mitochondrial dynamics observed in a wide range of conditions, from impaired development various neurodegenerative diseases. Novel imaging techniques genetic tools provide unprecedented access physiological roles by visualizing trafficking, morphological dynamics, generation, ultrastructure. Recent studies using these new have unveiled influence on axon branching, synaptic function, regulation with ER, glial cell neurogenesis, repair. This review provides overview crucial played CNS pathophysiological conditions.

Language: Английский

Citations

191
Maternal exposure to polystyrene nanoplastics causes brain abnormalities in progeny DOI
Bohyeon Jeong, Jeong Yeob Baek,

Jahong Koo

et al.

Journal of Hazardous Materials, Journal Year: 2021, Volume and Issue: 426, P. 127815 - 127815

Published: Nov. 18, 2021

Language: Английский

Citations

182
Oocytes maintain ROS-free mitochondrial metabolism by suppressing complex I DOI Creative Commons
Aida Rodríguez‐Nuevo, Ariadna Torres-Sanchez, Juan M. Durán

et al.

Nature, Journal Year: 2022, Volume and Issue: 607(7920), P. 756 - 761

Published: July 20, 2022

Oocytes form before birth and remain viable for several decades fertilization

Language: Английский

Citations

153
Proper acquisition of cell class identity in organoids allows definition of fate specification programs of the human cerebral cortex DOI Creative Commons
Ana Uzquiano, Amanda J. Kedaigle, Martina Pigoni

et al.

Cell, Journal Year: 2022, Volume and Issue: 185(20), P. 3770 - 3788.e27

Published: Sept. 1, 2022

Language: Английский

Citations

149
Dynamic profiling and functional interpretation of histone lysine crotonylation and lactylation during neural development DOI Open Access

Shang‐Kun Dai,

Peipei Liu,

Xiao Li

et al.

Development, Journal Year: 2022, Volume and Issue: 149(14)

Published: June 23, 2022

Metabolites such as crotonyl-CoA and lactyl-CoA influence gene expression by covalently modifying histones, known histone lysine crotonylation (Kcr) lactylation (Kla). However, the existence patterns, dynamic changes, biological functions associations of these modifications with acetylation during mammalian development remain largely unknown. Here, we find that Kcr Kla are widely distributed in brain undergo global changes neural development. By profiling genome-wide dynamics H3K9ac, H3K9cr H3K18la combination ATAC RNA sequencing, reveal marks tightly correlated chromatin state expression, extensively involved transcriptome remodeling to promote cell-fate transitions developing telencephalon. Importantly, demonstrate levels not consequence transcription identify deacetylases (HDACs) 1-3 novel 'erasers' H3K18la. Using P19 cells an induced differentiation system, HDAC1-3 inhibition MS-275 pre-activates neuronal transcriptional programs stimulating multiple acylations simultaneously. These findings suggest play crucial roles epigenetic regulation

Language: Английский

Citations

75
Mitochondrial pyruvate metabolism regulates the activation of quiescent adult neural stem cells DOI Creative Commons
Francesco Petrelli, Valentina Scandella,

Sylvie Montessuit

et al.

Science Advances, Journal Year: 2023, Volume and Issue: 9(9)

Published: March 1, 2023

Cellular metabolism is important for adult neural stem/progenitor cell (NSPC) behavior. However, its role in the transition from quiescence to proliferation not fully understood. We here show that mitochondrial pyruvate carrier (MPC) plays a crucial and unexpected part this process. MPC transports into mitochondria, linking cytosolic glycolysis tricarboxylic acid cycle oxidative phosphorylation. Despite metabolic key function, of NSPCs has been addressed. quiescent have an active express high levels MPC. Pharmacological inhibition increases aspartate triggers NSPC activation. Furthermore, genetic Mpc1 ablation vitro vivo also activates NSPCs, which differentiate mature neurons, leading overall increased hippocampal neurogenesis aged mice. These findings highlight importance regulation identify pathway through import controls

Language: Английский

Citations

52
Post-translational protein lactylation modification in health and diseases: a double-edged sword DOI Creative Commons
Hang Gong, Zhong Huang, Long Cheng

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Jan. 10, 2024

As more is learned about lactate, it acts as both a product and substrate functions shuttle system between different cell populations to provide the energy for sustaining tumor growth proliferation. Recent discoveries of protein lactylation modification mediated by lactate play an increasingly significant role in human health (e.g., neural osteogenic differentiation maturation) diseases tumors, fibrosis inflammation, etc.). These views are critically first described detail this review. Hence, here, we focused on new target, lactylation, which may be "double-edged sword" diseases. The main purpose review was describe how multiple physiological pathological processes their potential mechanisms through in-depth summary preclinical vitro vivo studies. Our work aims ideas treating accelerate translation from bench bedside.

Language: Английский

Citations

20
Recent mitochondria-immobilized fluorescent probes for high-fidelity bioimaging: From design to application DOI

Wenshu Meng,

Ran Chen,

Qingqing Jang

et al.

Coordination Chemistry Reviews, Journal Year: 2025, Volume and Issue: 529, P. 216456 - 216456

Published: Jan. 21, 2025

Language: Английский

Citations

5
The meninges host a distinct compartment of regulatory T cells that preserves brain homeostasis DOI
Miguel Marin-Rodero, Elisa Cintado, Alec J. Walker

et al.

Science Immunology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Our understanding of the meningeal immune system has recently burgeoned, particularly regarding how innate and adaptive effector cells are mobilized to meet brain challenges. However, information on immunocytes guard homeostasis in healthy individuals remains limited. This study highlights heterogeneous, polyfunctional regulatory T cell (T reg ) compartment meninges. A subtype specialized controlling interferon-gamma (IFN-γ) responses another dedicated regulating follicular B were substantial components this compartment. Accordingly, punctual ablation rapidly unleashed IFN-γ production by lymphocytes, unlocked access parenchyma, altered profiles. Distally, hippocampus assumed a reactive state, with morphological transcriptional changes multiple glial types. Within dentate gyrus, neural stem underwent more death blocked from further differentiation, which coincided impairments short-term spatial-reference memory. Thus, regs multifaceted safeguard at steady state.

Language: Английский

Citations

5