Nature Biotechnology, Journal Year: 2021, Volume and Issue: 40(3), P. 364 - 373
Published: Oct. 4, 2021
Language: Английский
Nature Biotechnology, Journal Year: 2023, Volume and Issue: 42(4), P. 617 - 627
Published: July 10, 2023
Abstract Whole-body imaging techniques play a vital role in exploring the interplay of physiological systems maintaining health and driving disease. We introduce wildDISCO, new approach for whole-body immunolabeling, optical clearing mice, circumventing need transgenic reporter animals or nanobody labeling so overcoming existing technical limitations. identified heptakis(2,6-di- O -methyl)-β-cyclodextrin as potent enhancer cholesterol extraction membrane permeabilization, enabling deep, homogeneous penetration standard antibodies without aggregation. WildDISCO facilitates peripheral nervous systems, lymphatic vessels immune cells whole mice at cellular resolution by diverse endogenous proteins. Additionally, we examined rare proliferating effects biological perturbations, demonstrated germ-free mice. applied wildDISCO to map tertiary lymphoid structures context breast cancer, considering both primary tumor metastases throughout mouse body. An atlas high-resolution images showcasing nervous, vascular is accessible http://discotechnologies.org/wildDISCO/atlas/index.php .
Language: Английский
Citations
71
Nature Immunology, Journal Year: 2023, Volume and Issue: 24(12), P. 1982 - 1993
Published: Nov. 27, 2023
Language: Английский
Citations
60
Science, Journal Year: 2024, Volume and Issue: 385(6713)
Published: Sept. 5, 2024
Optical imaging plays a central role in biology and medicine but is hindered by light scattering live tissue. We report the counterintuitive observation that strongly absorbing molecules can achieve optical transparency animals. explored physics behind this found when dissolve water, they modify refractive index of aqueous medium through Kramers-Kronig relations to match high-index tissue components such as lipids. have demonstrated our straightforward approach reversibly render mouse body transparent allow visualization wide range deep-seated structures activities. This work suggests search for high-performance clearing agents should focus on molecules.
Language: Английский
Citations
47
Opto-Electronic Advances, Journal Year: 2024, Volume and Issue: 7(6), P. 230212 - 230212
Published: Jan. 1, 2024
Language: Английский
Citations
20
Bioengineering & Translational Medicine, Journal Year: 2024, Volume and Issue: 9(2)
Published: Jan. 20, 2024
Abstract In this review, we explore the growing role of artificial intelligence (AI) in advancing biomedical applications human pluripotent stem cell (hPSC)‐derived organoids. Stem cell‐derived organoids, these miniature organ replicas, have become essential tools for disease modeling, drug discovery, and regenerative medicine. However, analyzing vast intricate datasets generated from organoids can be inefficient error‐prone. AI techniques offer a promising solution to efficiently extract insights make predictions diverse data types microscopy images, transcriptomics, metabolomics, proteomics. This review offers brief overview organoid characterization fundamental concepts while focusing on comprehensive exploration organoid‐based modeling evaluation. It provides into future possibilities enhancing quality control fabrication, label‐free recognition, three‐dimensional image reconstruction complex structures. presents challenges potential solutions AI‐organoid integration, establishment reliable model decision‐making processes standardization research.
Language: Английский
Citations
18
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: March 27, 2024
Abstract In 2015, we launched the mesoSPIM initiative, an open-source project for making light-sheet microscopy of large cleared tissues more accessible. Meanwhile, demand imaging larger samples at higher speed and resolution has increased, requiring major improvements in capabilities such microscopes. Here, introduce next-generation (“Benchtop”) with a significantly increased field view, improved resolution, throughput, affordable cost, simpler assembly compared to original version. We develop optical method testing detection objectives that enables us select optimal large-sensor cameras. The achieves high spatial (1.5 µm laterally, 3.3 axially) across entire magnification up 20×, supports sample sizes ranging from sub-mm several centimeters while being compatible multiple clearing techniques. microscope serves broad range applications neuroscience, developmental biology, pathology, even physics.
Language: Английский
Citations
17
European Journal of Nuclear Medicine and Molecular Imaging, Journal Year: 2021, Volume and Issue: 48(8), P. 2338 - 2350
Published: Feb. 13, 2021
Abstract Pathology is the medical specialty concerned with study of disease nature and causes, playing a key role in bridging basic researches clinical medicine. In course development, pathology has significantly expanded our understanding disease, exerted enormous impact on management patients. However, challenges facing pathology, inherent invasiveness pathological practice persistent concerns sample representativeness, constitute its limitations. Molecular imaging noninvasive technique to visualize, characterize, measure biological processes at molecular level living subjects. With continuous development equipment probes, enabled an increasingly precise evaluation pathophysiological changes. A new pathophysiology visualization system based forming shows great potential reform practice. Several improvements “trans-,” including trans-scale, transparency, translation, would be driven by this kind Pathological changes could evaluated trans-scale mode; tissues transparentized better present underlying information; translational research facilitated. Thus, transpathology greatly facilitate deciphering events multiscale perspective, supporting precision medicine future.
Language: Английский
Citations
103
Nature Protocols, Journal Year: 2020, Volume and Issue: 16(1), P. 239 - 262
Published: Nov. 27, 2020
Language: Английский
Citations
86
Cell Reports, Journal Year: 2020, Volume and Issue: 33(5), P. 108349 - 108349
Published: Nov. 1, 2020
We present a tiling light sheet microscope compatible with all tissue clearing methods for rapid multicolor 3D imaging of cleared tissues micron-scale (4 × 4 10 μm3) to submicron-scale (0.3 0.3 1 spatial resolution. The resolving ability is improved sub-100 nm (70 70 200 nm3) via expansion. uses sheets achieve higher resolution and better optical sectioning than conventional microscopes. illumination phase modulated adjust the position intensity profile based on desired speed keep aligned. align modulation alone also ensures its accuracy makes reliable easy operate. Here we describe working principle design microscope. demonstrate utility by various tissues.
Language: Английский
Citations
81
European Journal of Immunology, Journal Year: 2021, Volume and Issue: 51(5), P. 1262 - 1277
Published: Feb. 8, 2021
Abstract Multiparameter tissue imaging enables analysis of cell‐cell interactions in situ, the cellular basis for structure, and novel cell types that are spatially restricted, giving clues to biological mechanisms behind homeostasis disease. Here, we streamlined simplified multiplexed method CO‐Detection by indEXing (CODEX) validating 58 unique oligonucleotide barcodes can be conjugated antibodies. We showed barcoded antibodies retained their specificity staining cognate targets human tissue. Antibodies were visualized one at a time adding fluorescently labeled complementary barcode, imaging, stripping, repeating this cycle. With developed panel 46 was used stain five lymphoid tissues: three tonsils, spleen, LN. To analyze data produced, an image processing pipeline enabled single‐cell on data, including unsupervised clustering, revealed 31 across all tissues. compared cell‐type compositions within directly surrounding follicles from different organs evaluated density correlations. This sequential exchange technique facile tissues leverages pre‐existing infrastructure decrease barriers broad use imaging.
Language: Английский
Citations
79