Journal of Boron,
Journal Year:
2025,
Volume and Issue:
10(1), P. 1 - 9
Published: April 1, 2025
This
study
designed
to
investigate
the
biochemical
changes
in
glial
cells'
oxidant/antioxidant
systems
response
glutamate-induced
excitotoxicity
of
boric
acid
(BA).
The
present
employed
C6
cells.
For
study,
cells
were
separated
into
4
groups
as
control,
glutamate
(10mM),
glutamate+BA
(0,23;
0,46;
0,93;
1,87
and
3,75
μg/mL),
BA
μg/mL).
control
group
was
not
treated.
treated
with
10
mM
for
24
hours.
administered
one
hour
prior
addition
incubated
viability
evaluated
using
an
XTT
assay.
Commercial
kits
used
analyses.
Significance
set
at
less
than
0.05.
analysis
revealed
that
levels
malondialdehyde
(MDA),
nitric
oxide
(NO),
inducible
snythase
(iNOS),
neuronal
synthase
(nNOS),
total
oxidant
status
(TOS)
elevated
compared
(p
APOPTOSIS,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 17, 2025
Abstract
Neuronal
damage
in
brain
ischemia
is
characterized
by
a
disassembly
of
the
proteasome
and
decrease
its
proteolytic
activity.
However,
to
what
extent
these
alterations
are
coupled
neuronal
death
controversial
since
inhibitors
were
shown
provide
protection
different
models
stroke
rodents.
This
question
was
addressed
present
work
using
cultured
rat
cerebrocortical
neurons
subjected
transient
oxygen-
glucose-deprivation
(OGD)
as
model
for
vitro
ischemia.
Under
latter
conditions
there
time-dependent
loss
activity,
determined
cleavage
Suc-LLVY-AMC
fluorogenic
substrate,
proteasome,
assessed
native-polyacrylamide
gel
electrophoresis
followed
western
blot
against
Psma2
Rpt6,
which
components
catalytic
core
regulatory
particle,
respectively.
Immunocytochemistry
experiments
two
proteins
also
showed
differential
effects
on
their
dendritic
distribution.
OGD
downregulated
protein
levels
Rpt3
Rpt10,
mechanism
dependent
activity
NMDA
receptors
mediated
calpains.
Activation
an
inhibitor
USP14,
deubiquitinase
enzyme,
inhibited
OGD-induced
cell
death,
decreased
calpain
analysis
spectrin
cleavage.
Similar
results
obtained
presence
oleic
amide
derivatives
(B12
D3)
directly
activate
20S
particle.
Together,
show
that
activation
prevents
cortical
ischemia,
indicating
inhibition
mediator
Journal of Boron,
Journal Year:
2025,
Volume and Issue:
10(1), P. 1 - 9
Published: April 1, 2025
This
study
designed
to
investigate
the
biochemical
changes
in
glial
cells'
oxidant/antioxidant
systems
response
glutamate-induced
excitotoxicity
of
boric
acid
(BA).
The
present
employed
C6
cells.
For
study,
cells
were
separated
into
4
groups
as
control,
glutamate
(10mM),
glutamate+BA
(0,23;
0,46;
0,93;
1,87
and
3,75
μg/mL),
BA
μg/mL).
control
group
was
not
treated.
treated
with
10
mM
for
24
hours.
administered
one
hour
prior
addition
incubated
viability
evaluated
using
an
XTT
assay.
Commercial
kits
used
analyses.
Significance
set
at
less
than
0.05.
analysis
revealed
that
levels
malondialdehyde
(MDA),
nitric
oxide
(NO),
inducible
snythase
(iNOS),
neuronal
synthase
(nNOS),
total
oxidant
status
(TOS)
elevated
compared
(p
