Published: Jan. 1, 2025
Language: Английский
Current Biology, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Language: Английский
Citations
1
Current Biology, Journal Year: 2025, Volume and Issue: 35(5), P. R164 - R168
Published: March 1, 2025
Language: Английский
Citations
1
iScience, Journal Year: 2025, Volume and Issue: 28(4), P. 112194 - 112194
Published: March 11, 2025
Recent studies have revealed diverse neuron types in the superior colliculus (SC), a midbrain structure critical for sensorimotor transformation. Here, as an important step toward studying function of these subtypes, we characterize 10 transgenic mouse lines based on recently published molecular atlas superficial SC. We show that Cre or fluorescence expression some corresponds specifically to certain transcriptomic types. These include two GENSAT been used target morphological cell SC and three knockin lines. In contrast, such correspondence is not seen other tested mice. Importantly, pattern marker genes all highly consistent with atlas. Together, our support correlation between types, identify useful targeting genetically, demonstrate validity single-cell transcriptomics data.
Language: Английский
Citations
1
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 13, 2025
Abstract Locating the source of a specific sound in complex environment and determining its saliency is critical for survival. The superior colliculus (SC), sensorimotor midbrain structure, plays an important role localization has been shown to have topographic map auditory space range species. In mice, previous studies using broadband white noise stimuli found that neurons use high-frequency monaural spectral cues interaural level differences (ILDs) compute spatially restricted receptive fields (RFs), these RFs are organized topographically along azimuth. However, naturalistic environment, animal encounters may rich components; however, sources can still be localized efficiently. It remains unknown whether how SC respond sounds and, turn, RF. Here, we show results from large-scale vivo physiological recordings response noise, ultrasonic pup calls chirps. We find mouse with distinct temporal patterns spatial preference predominantly at ∼60 degrees contralateral addition, categorized based on their spectrotemporal field demonstrated there least 4 subtypes responsive neurons. Significance Statement (SC) receives visual information used localize objects. While organization composition visually well described, much less known about types properties presented calls, chirp mice while recording Analysis neuronal responses defines classes also stimuli, mainly occur when side but not front animal. These lead hypothesis different strategies depending source.
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 23, 2025
The superior colliculus (SC), a midbrain sensorimotor hub, is anatomically and functionally similar across vertebrates, but how its cell types have evolved unclear. Using single-nucleus transcriptomics, we compared the SC's molecular cellular organization in mice, tree shrews, humans. Despite over 96 million years of evolutionary divergence, identified ∼30 consensus neuronal subtypes, including Cbln2 + neurons that form SC-pulvinar circuit mice shrews. Synapse-related genes were among most conserved, unlike neocortex, suggesting co-conservation synaptic circuitry. In contrast, cilia-related diverged significantly species, highlighting potential importance primary cilium SC evolution. Additionally, novel inhibitory neuron shrews humans not mice. Our findings reveal has by conserving genes, circuitry, while diversifying ciliary gene expression an subtype.
Language: Английский
Citations
0
Published: Jan. 1, 2025
Language: Английский
Citations
0