Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Dec. 28, 2024
Abstract
Osteoarthritis
(OA)
is
a
complex
disease
of
cartilage
characterised
by
joint
pain,
functional
limitation,
and
reduced
quality
life
with
affected
movement
leading
to
pain
limited
mobility.
Current
methods
diagnose
OA
are
predominantly
X-ray,
MRI
invasive
fluid
analysis,
all
which
lack
chemical
or
molecular
specificity
detection
the
at
later
stages.
A
rapid
minimally
non-destructive
approach
diagnosis
critical
unmet
need.
Label-free
techniques
such
as
Raman
Spectroscopy
(RS),
Coherent
anti-Stokes
scattering
(CARS),
Second
Harmonic
Generation
(SHG)
Two
Photon
Fluorescence
(TPF)
increasingly
being
used
characterise
tissue.
However,
current
studies
based
on
whole
tissue
analysis
do
not
consider
different
structurally
distinct
layers
in
cartilage.
In
this
work,
we
use
spectroscopy
obtain
signatures
from
superficial
(top)
deep
(bottom)
layer
healthy
osteoarthritic
samples
64
patients
(19
control
45
OA).
Spectra
were
acquired
both
‘fingerprint’
region
700
1720
cm
−
1
high-frequency
stretching
2500
3300
.
Principal
component
linear
discriminant
was
identify
peaks
that
contributed
significantly
classification
accuracy
samples.
The
most
pronounced
differences
observed
proline
(855
921
)
hydroxyproline
(877
938
),
sulphated
glycosaminoglycan
(sGAG)
(1064
1380
frequencies
for
well
1245
1272
,
1320
1345
1451
collagen
modes
altered
samples,
consistent
expected
structural
changes.
Classification
fingerprint
spectral
controls
found
be
97%
93%
using
individual/all
spectra
and,
100%
95%
mean
per
patient,
respectively.
diseased
classified
an
88%
84%
spectra,
96%
patient
layers,
C-H
(2500–3300
resulted
high
identification
but
low
controls.
Differential
changes
age
(under
60
over
years),
contrast,
less
significant
gender.
Prominent
preliminarily
imaged
CARS,
SHG
TPF.
Cell
clustering
together
pericellular
matrix
structure
correlating
analysis.
study
demonstrates
potential
multimodal
imaging
interrogate
provides
insight
into
composition
its
implications
increasing
aging
demographic.
Tissue Engineering Part A,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 15, 2025
Cartilage
injuries
are
extremely
common
in
the
general
population,
and
conventional
interventions
have
failed
to
produce
optimal
results.
Tissue
engineering
(TE)
technology
has
been
developed
neocartilage
for
use
a
variety
of
cartilage-related
conditions.
However,
progress
field
cartilage
TE
historically
difficult
due
high
functional
demand
avascular
nature
tissue.
Recent
advancements
cell
sourcing,
biostimulation,
scaffold
revolutionized
made
clinical
application
this
reality.
will
continue
expand
its
horizons
fully
integrate
three-dimensional
printing,
gene
editing,
sourcing
future.
This
review
focuses
on
recent
landscape
treatments
conditions.AbstractImpact
StatementCartilage
tissue
potential
transform
practice
through
swift
generation
integrative
neotissues.
There
is
an
increasing
effective
therapies
conditions
such
as
osteoarthritis
focal
lesions,
scientific
breakthroughs
brought
closer
widespread
use.
with
emphasis
applications
that
can
further
enhance
contribute
improved
care
patients.
Journal of Biomedical Materials Research Part B Applied Biomaterials,
Journal Year:
2025,
Volume and Issue:
113(2)
Published: Jan. 22, 2025
ABSTRACT
Intervertebral
disc
degeneration
(IDD)
is
one
of
the
leading
causes
chronic
pain
and
disability,
traditional
treatment
methods
often
struggle
to
restore
its
complex
biomechanical
properties.
This
article
explores
innovative
application
self‐healing
hydrogels
in
IDD,
offering
new
hope
for
repair
due
their
exceptional
self‐repair
capabilities
adaptability.
As
a
key
support
structure
human
body,
intervertebral
discs
are
damaged
by
trauma
or
degenerative
changes.
Self‐healing
not
only
mimic
mechanical
properties
natural
but
also
when
damaged,
thereby
maintaining
stable
functionality.
reviews
mechanisms
design
strategies
and,
first
time,
outlines
potential
IDD.
Furthermore,
looks
forward
future
developments
field,
including
intelligent
material
design,
multifunctional
integration,
encapsulation
release
bioactive
molecules,
combinations
with
tissue
engineering
stem
cell
therapy,
perspectives
IDD
treatment.
Advanced Healthcare Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 30, 2025
Abstract
Cartilage
regeneration
requires
a
specialized
biomechanical
environment.
Macroscopically,
cartilage
repair
protracted,
stable
mechanical
environment,
whereas
microscopically,
it
involves
dynamic
interactions
between
cells
and
the
extracellular
matrix.
Therefore,
this
study
aims
to
design
hydrogel
that
meets
complex
requirements
for
repair.
Dynamic
hybrid
hydrogels
with
temporal
stability
at
macroscale
properties
microscale
are
successfully
synthesized.
The
simulates
stress
relaxation
viscoelasticity
of
pericellular
matrix,
facilitating
effective
matrix
cells.
in
vitro
vivo
experiments
demonstrated
significantly
promoted
alleviates
abnormal
actin
polymerization,
reduces
intracellular
stress,
increases
volume
individual
By
modulating
cytoskeleton,
inhibits
Notch
signal
transduction
both
receptor
ligand
cells,
resulting
an
improved
phenotype.
This
introduces
scaffold
modulates
chondrocyte
cytoskeleton
signaling
pathways
by
establishing
appropriate
thus
offering
promising
material
Research Square (Research Square),
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 15, 2025
Abstract
The
body
has
evolved
three
types
of
cartilage:
hyaline,
elastic,
and
fibrocartilage.
Modern
tissue
engineering
techniques
can
harvest
different
chondrocytes,
expand
them
in
vitro,
use
to
repair
various
cartilage
defects.
However,
the
modulatory
effect
cartilaginous
niches
on
type
regenerated
after
implantation
chondrocytes
from
origins
remains
unknown.
In
this
study,
typical
cartilage—auricular
(elastic
cartilage),
articular
(hyaline
meniscus
(fibrocartilage)—were
investigated.
Chondrocytes
derived
these
cartilages
were
mixed
with
Pluronic
gel
implanted
into
for
one
month.
Our
results
demonstrated
that
environment,
auricular
lost
elastin
expression,
lacked
a
fibrous
structure,
showing
reduced
I
collagen
increased
II
all
resembling
hyaline
cartilage-like
structure.
did
not
express
elastin,
maintaining
while
fibrocartilage
failed
form
cartilage.
exhibit
weak
expression
positive
expression.
Regenerated
transform
This
study
provides
valuable
insights
how
influence
characteristics
cartilage,
offering
potential
implications
improving
strategies
engineering.
Tissue Engineering Part B Reviews,
Journal Year:
2024,
Volume and Issue:
unknown
Published: July 6, 2024
Cartilage
tissue,
encompassing
hyaline
cartilage,
fibrocartilage,
and
elastic
plays
a
pivotal
role
in
the
human
body
because
of
its
unique
composition,
structure,
biomechanical
properties.
However,
inherent
avascularity
limited
regenerative
capacity
cartilage
present
significant
challenges
to
healing
following
injury.
This
review
provides
comprehensive
analysis
current
state
tissue
engineering,
focusing
on
critical
components
cell
sources,
scaffolds,
growth
factors
tailored
regeneration
each
type.
We
explore
similarities
differences
properties
three
types
their
implications
for
engineering.
A
emphasis
is
placed
innovative
strategies
regeneration,
including
potential
