Harnessing Raman spectroscopy and multimodal imaging of cartilage for osteoarthritis diagnosis DOI Creative Commons
Anna Crisford,

Hiroki Cook,

Konstantinos N. Bourdakos

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 28, 2024

Abstract Osteoarthritis (OA) is a complex disease of cartilage characterised by joint pain, functional limitation, and reduced quality life with affected movement leading to pain limited mobility. Current methods diagnose OA are predominantly X-ray, MRI invasive fluid analysis, all which lack chemical or molecular specificity detection the at later stages. A rapid minimally non-destructive approach diagnosis critical unmet need. Label-free techniques such as Raman Spectroscopy (RS), Coherent anti-Stokes scattering (CARS), Second Harmonic Generation (SHG) Two Photon Fluorescence (TPF) increasingly being used characterise tissue. However, current studies based on whole tissue analysis do not consider different structurally distinct layers in cartilage. In this work, we use spectroscopy obtain signatures from superficial (top) deep (bottom) layer healthy osteoarthritic samples 64 patients (19 control 45 OA). Spectra were acquired both ‘fingerprint’ region 700 1720 cm − 1 high-frequency stretching 2500 3300 . Principal component linear discriminant was identify peaks that contributed significantly classification accuracy samples. The most pronounced differences observed proline (855 921 ) hydroxyproline (877 938 ), sulphated glycosaminoglycan (sGAG) (1064 1380 frequencies for well 1245 1272 , 1320 1345 1451 collagen modes altered samples, consistent expected structural changes. Classification fingerprint spectral controls found be 97% 93% using individual/all spectra and, 100% 95% mean per patient, respectively. diseased classified an 88% 84% spectra, 96% patient layers, C-H (2500–3300 resulted high identification but low controls. Differential changes age (under 60 over years), contrast, less significant gender. Prominent preliminarily imaged CARS, SHG TPF. Cell clustering together pericellular matrix structure correlating analysis. study demonstrates potential multimodal imaging interrogate provides insight into composition its implications increasing aging demographic.

Language: Английский

Exploring advancements in polysaccharide-based approaches: The cornerstone of next-generation cartilage regeneration therapeutics DOI
Noella Abraham,

Giriraj Pandey,

Tejaswini Kolipaka

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 141352 - 141352

Published: Feb. 1, 2025

Language: Английский

Citations

2
Injectable biomimetic hydrogel based on modified chitosan and silk fibroin with decellularized cartilage extracellular matrix for cartilage repair and regeneration DOI

Jinpeng Wan,

Jinshan Jiang,

Xinyi Yu

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 140058 - 140058

Published: Jan. 1, 2025

Language: Английский

Citations

1
Restoring articular cartilage: insights from structure, composition and development DOI
Alba Pueyo-Moliner, Keita Ito, Frank Zaucke

et al.

Nature Reviews Rheumatology, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

Language: Английский

Citations

1
Current Concepts and Clinical Applications in Cartilage Tissue Engineering DOI
C Wright,

Serafina Faith Zotter,

Wei Shao Tung

et al.

Tissue Engineering Part A, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Cartilage injuries are extremely common in the general population, and conventional interventions have failed to produce optimal results. Tissue engineering (TE) technology has been developed neocartilage for use a variety of cartilage-related conditions. However, progress field cartilage TE historically difficult due high functional demand avascular nature tissue. Recent advancements cell sourcing, biostimulation, scaffold revolutionized made clinical application this reality. will continue expand its horizons fully integrate three-dimensional printing, gene editing, sourcing future. This review focuses on recent landscape treatments conditions.AbstractImpact StatementCartilage tissue potential transform practice through swift generation integrative neotissues. There is an increasing effective therapies conditions such as osteoarthritis focal lesions, scientific breakthroughs brought closer widespread use. with emphasis applications that can further enhance contribute improved care patients.

Language: Английский

Citations

0
Application of Self‐Healing Hydrogels in the Treatment of Intervertebral Disc Degeneration DOI Open Access
Cunliang Guo, Xinyi Jiao,

Xiaoxun Du

et al.

Journal of Biomedical Materials Research Part B Applied Biomaterials, Journal Year: 2025, Volume and Issue: 113(2)

Published: Jan. 22, 2025

ABSTRACT Intervertebral disc degeneration (IDD) is one of the leading causes chronic pain and disability, traditional treatment methods often struggle to restore its complex biomechanical properties. This article explores innovative application self‐healing hydrogels in IDD, offering new hope for repair due their exceptional self‐repair capabilities adaptability. As a key support structure human body, intervertebral discs are damaged by trauma or degenerative changes. Self‐healing not only mimic mechanical properties natural but also when damaged, thereby maintaining stable functionality. reviews mechanisms design strategies and, first time, outlines potential IDD. Furthermore, looks forward future developments field, including intelligent material design, multifunctional integration, encapsulation release bioactive molecules, combinations with tissue engineering stem cell therapy, perspectives IDD treatment.

Language: Английский

Citations

0
Editorial: K. A. Athanasiou Special Issue DOI
Farshid Guilak, Michael S. Detamore, Gabriela Espinosa

et al.

Tissue Engineering Part A, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

Language: Английский

Citations

0
Injectable Microspheres With Cartilage‐Like Structure Facilitate Inflammation Inhibition and Tissue Regeneration DOI Open Access
Qi Feng, Qingtao Li,

Zequ Lin

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 16, 2025

Abstract The unique structure of cartilage tissue, characterized by a robust collagen fiber framework and dynamic proteoglycan hydrogel filling, is crucial for maintaining biomechanical biochemical homeostasis. Herein, cartilage‐inspired microsphere with sponge filling (SponGel MS) successfully prepared to restore Specifically, epigallocatechin gallate nanoparticles coated MnO 2 (MnO @EGCG) are first synthesized, which can scavenge H O hydroxyl radicals. formation SponGel MS involves sequential fabrication the phase through cryogenic radical reaction via Schiff base reaction. dynamically release @EGCG inhibit inflammation SDF‐1 recruit stem cells. Porous favorable compressive performance His‐Ala‐Val (HAV) peptide provides stable cell niches regulate condensation, co‐assembly chondrogenic differentiation. Animal experiments demonstrate that suppresses expression IL‐1β I, ultimately achieving regeneration. This study represents novel strategy constructing repair materials.

Language: Английский

Citations

0
Application of Engineered Stem Cells in the Field of Tissue Regeneration DOI Open Access

佳 王

Journal of Clinical Personalized Medicine, Journal Year: 2025, Volume and Issue: 04(01), P. 977 - 984

Published: Jan. 1, 2025

Language: Английский

Citations

0
Stable‐Dynamic Hydrogels Mimicking the Pericellular Matrix for Articular Cartilage Repair DOI Open Access
Yixin Li, Tian‐Cheng Li, Cheng Zhu

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 30, 2025

Abstract Cartilage regeneration requires a specialized biomechanical environment. Macroscopically, cartilage repair protracted, stable mechanical environment, whereas microscopically, it involves dynamic interactions between cells and the extracellular matrix. Therefore, this study aims to design hydrogel that meets complex requirements for repair. Dynamic hybrid hydrogels with temporal stability at macroscale properties microscale are successfully synthesized. The simulates stress relaxation viscoelasticity of pericellular matrix, facilitating effective matrix cells. in vitro vivo experiments demonstrated significantly promoted alleviates abnormal actin polymerization, reduces intracellular stress, increases volume individual By modulating cytoskeleton, inhibits Notch signal transduction both receptor ligand cells, resulting an improved phenotype. This introduces scaffold modulates chondrocyte cytoskeleton signaling pathways by establishing appropriate thus offering promising material

Language: Английский

Citations

0
Eutectogels: Recent Advances and Emerging Biological Applications DOI
Yi Hu, Wenhui Yang,

Jiamian Zhan

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Abstract Eutectogels, a family of emerging materials, integrate the characteristics deep eutectic solvents (DES) and gels, presenting unique application potential in biomedical engineering. However, latest progress eutectogels their applications field remain to be summarized refined. This review first introduces composition properties generated by interaction between DES gel matrix. Subsequently, classifications advantages are comprehensively discussed according cross‐linked preparation methods, including physical, chemical, multiple cross‐linked. By analyzing eutectogels, such as mechanical performance, thermal properties, conductivity, injectability, structure‐function relationships, is overviewed. Then, eutectogel's prospects engineering elaborated detail, drug delivery improve stability precise control release, enhancing curative effects reducing side effects. Also, functional utilization tissue engineering, biosensing, bioadhesives, related domains explored. Finally, existing challenges prospective research directions delineated.

Language: Английский

Citations

0