Search-and-replace genome editing without double-strand breaks or donor DNA

Nature, Journal Year: 2019, Volume and Issue: 576(7785), P. 149 - 157

Published: Oct. 21, 2019

Language: Английский

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Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors

Nature Biotechnology, Journal Year: 2020, Volume and Issue: 38(7), P. 824 - 844

Published: June 22, 2020

Language: Английский

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CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing

Nucleic Acids Research, Journal Year: 2019, Volume and Issue: 47(W1), P. W171 - W174

Published: May 6, 2019

Abstract The CRISPR–Cas system is a powerful genome editing tool that functions in diverse array of organisms and cell types. technology was initially developed to induce targeted mutations DNA, but has now been adapted target nucleic acids for range purposes. CHOPCHOP web identifying single guide RNA (sgRNA) targets. In this major update CHOPCHOP, we expand our toolbox beyond knockouts. We introduce functionality targeting with Cas13, which includes support alternative transcript isoforms accessibility predictions. incorporate new DNA modes, including CRISPR activation/repression, enrichment loci long-read sequencing, prediction Cas9 repair outcomes. Finally, results page visualization reveal downstream ATG sites, will aid users avoiding the expression truncated proteins. supports over 200 genomes have released command-line script running larger jobs handling unsupported genomes. v3 can be found at https://chopchop.cbu.uib.no

Language: Английский

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CRISPR-Based Therapeutic Genome Editing: Strategies and In Vivo Delivery by AAV Vectors

Cell, Journal Year: 2020, Volume and Issue: 181(1), P. 136 - 150

Published: April 1, 2020

Language: Английский

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Unbiased detection of CRISPR off-targets in vivo using DISCOVER-Seq

Science, Journal Year: 2019, Volume and Issue: 364(6437), P. 286 - 289

Published: April 19, 2019

Spotting off-targets from gene editing Unintended genomic modifications limit the potential therapeutic use of gene-editing tools. Available methods to find generally do not work in vivo or detect single-nucleotide changes. Three papers this issue report new for monitoring tools (see Perspective by Kempton and Qi). Wienert et al. followed recruitment a DNA repair protein breaks induced CRISPR-Cas9, enabling unbiased detection off-target cellular animal models. Zuo identified without interference natural genetic heterogeneity injecting base editors into one blastomere two-cell mouse embryo leaving other genetically identical unedited. Jin performed whole-genome sequencing on individual, genome-edited rice plants identify unintended mutations. Cytosine, but adenine, numerous variants both rice. Science , p. 286 289 292 ; see also 234

Language: Английский

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Advances in genome editing through control of DNA repair pathways

Nature Cell Biology, Journal Year: 2019, Volume and Issue: 21(12), P. 1468 - 1478

Published: Dec. 1, 2019

Language: Английский

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Prime editing for precise and highly versatile genome manipulation

Nature Reviews Genetics, Journal Year: 2022, Volume and Issue: 24(3), P. 161 - 177

Published: Nov. 7, 2022

Language: Английский

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The molecular basis and disease relevance of non-homologous DNA end joining

Nature Reviews Molecular Cell Biology, Journal Year: 2020, Volume and Issue: 21(12), P. 765 - 781

Published: Oct. 19, 2020

Language: Английский

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Continuous evolution of SpCas9 variants compatible with non-G PAMs

Nature Biotechnology, Journal Year: 2020, Volume and Issue: 38(4), P. 471 - 481

Published: Feb. 10, 2020

Language: Английский

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DNA Repair Pathway Choices in CRISPR-Cas9-Mediated Genome Editing

Trends in Genetics, Journal Year: 2021, Volume and Issue: 37(7), P. 639 - 656

Published: April 22, 2021

Language: Английский

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