Nature Genetics,
Journal Year:
2021,
Volume and Issue:
53(4), P. 487 - 499
Published: April 1, 2021
Abstract
The
relationship
between
chromatin
organization
and
gene
regulation
remains
unclear.
While
disruption
of
domains
domain
boundaries
can
lead
to
misexpression
developmental
genes,
acute
depletion
regulators
genome
has
a
relatively
small
effect
on
expression.
It
is
therefore
uncertain
whether
expression
state
drive
or
changes
in
facilitate
cell-type-specific
activation
Here,
using
the
dorsoventral
patterning
Drosophila
melanogaster
embryo
as
model
system,
we
provide
evidence
for
independence
We
define
tissue-specific
enhancers
link
them
patterns
single-cell
RNA-seq.
Surprisingly,
despite
states
expression,
largely
maintained
across
tissues.
Our
results
indicate
that
conformation
not
necessary
but
rather
acts
scaffold
facilitating
when
become
active.
Science,
Journal Year:
2019,
Volume and Issue:
365(6457)
Published: Sept. 5, 2019
The
complex
three-dimensional
organization
of
genomes
in
the
cell
nucleus
arises
from
a
wide
range
architectural
features
including
DNA
loops,
chromatin
domains,
and
higher-order
compartments.
Although
these
are
universally
present
most
types
tissues,
recent
single-cell
biochemistry
imaging
approaches
have
demonstrated
stochasticity
transcription
high
variability
architecture
individual
cells.
We
review
occurrence,
mechanistic
basis,
functional
implications
genome
organization.
summarize
observations
on
cell-
allele-specific
architecture,
discuss
nature
extrinsic
intrinsic
sources
organization,
highlight
potential
structural
heterogeneity
for
function.
