AI in health and medicine

Nature Medicine, Journal Year: 2022, Volume and Issue: 28(1), P. 31 - 38

Published: Jan. 1, 2022

Language: Английский

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Diversity and Biology of Cancer-Associated Fibroblasts

Physiological Reviews, Journal Year: 2020, Volume and Issue: 101(1), P. 147 - 176

Published: May 29, 2020

Efforts to develop anti-cancer therapies have largely focused on targeting the epithelial compartment, despite presence of non-neoplastic stromal components that substantially contribute progression tumor. Indeed, cancer cell survival, growth, migration, and even dormancy are influenced by surrounding tumor microenvironment (TME). Within TME, cancer-associated fibroblasts (CAFs) been shown play several roles in development a They secrete growth factors, inflammatory ligands, extracellular matrix proteins promote proliferation, therapy resistance, immune exclusion. However, recent work indicates CAFs may also restrain some circumstances. In this review, we summarize body CAFs, with particular focus most discoveries about fibroblast heterogeneity, plasticity, functions. We highlight commonalities present across different types, normal states. Finally, latest advances regarding therapeutic strategies undergoing preclinical clinical evaluation.

Language: Английский

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Treatment landscape of triple-negative breast cancer — expanded options, evolving needs

Nature Reviews Clinical Oncology, Journal Year: 2021, Volume and Issue: 19(2), P. 91 - 113

Published: Nov. 9, 2021

Language: Английский

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Coordinated Cellular Neighborhoods Orchestrate Antitumoral Immunity at the Colorectal Cancer Invasive Front

Cell, Journal Year: 2020, Volume and Issue: 182(5), P. 1341 - 1359.e19

Published: Aug. 6, 2020

Antitumoral immunity requires organized, spatially nuanced interactions between components of the immune tumor microenvironment (iTME). Understanding this coordinated behavior in effective versus ineffective control will advance immunotherapies. We re-engineered co-detection by indexing (CODEX) for paraffin-embedded tissue microarrays, enabling simultaneous profiling 140 regions from 35 advanced-stage colorectal cancer (CRC) patients with 56 protein markers. identified nine conserved, distinct cellular neighborhoods (CNs)—a collection characteristic CRC iTME. Enrichment PD-1+CD4+ T cells only within a granulocyte CN positively correlated survival high-risk patient subset. Coupling and CNs, fragmentation cell macrophage disruption inter-CN communication was associated inferior outcomes. This study provides framework interrogating how complex biological processes, such as antitumoral immunity, occur through concerted actions spatial domains.

Language: Английский

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Giotto: a toolbox for integrative analysis and visualization of spatial expression data

Genome biology, Journal Year: 2021, Volume and Issue: 22(1)

Published: March 8, 2021

Abstract Spatial transcriptomic and proteomic technologies have provided new opportunities to investigate cells in their native microenvironment. Here we present Giotto, a comprehensive open-source toolbox for spatial data analysis visualization. The module provides end-to-end by implementing wide range of algorithms characterizing tissue composition, expression patterns, cellular interactions. Furthermore, single-cell RNAseq can be integrated cell-type enrichment analysis. visualization allows users interactively visualize outputs imaging features. To demonstrate its general applicability, apply Giotto datasets encompassing diverse platforms.

Language: Английский

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Squidpy: a scalable framework for spatial omics analysis

Nature Methods, Journal Year: 2022, Volume and Issue: 19(2), P. 171 - 178

Published: Jan. 31, 2022

Spatial omics data are advancing the study of tissue organization and cellular communication at an unprecedented scale. Flexible tools required to store, integrate visualize large diversity spatial data. Here, we present Squidpy, a Python framework that brings together from image analysis enable scalable description molecular data, such as transcriptome or multivariate proteins. Squidpy provides efficient infrastructure numerous methods allow efficiently manipulate interactively is extensible can be interfaced with variety already existing libraries for

Language: Английский

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Whole-cell segmentation of tissue images with human-level performance using large-scale data annotation and deep learning

Nature Biotechnology, Journal Year: 2021, Volume and Issue: 40(4), P. 555 - 565

Published: Nov. 18, 2021

Language: Английский

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A single-cell map of intratumoral changes during anti-PD1 treatment of patients with breast cancer

Nature Medicine, Journal Year: 2021, Volume and Issue: 27(5), P. 820 - 832

Published: May 1, 2021

Language: Английский

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Non-genetic mechanisms of therapeutic resistance in cancer

Nature reviews. Cancer, Journal Year: 2020, Volume and Issue: 20(12), P. 743 - 756

Published: Oct. 8, 2020

Language: Английский

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Wnt signaling in breast cancer: biological mechanisms, challenges and opportunities

Molecular Cancer, Journal Year: 2020, Volume and Issue: 19(1)

Published: Nov. 24, 2020

Abstract Wnt signaling is a highly conserved pathway that plays critical role in controlling embryonic and organ development, as well cancer progression. Genome-wide sequencing gene expression profile analyses have demonstrated involved mainly the processes of breast proliferation metastasis. The most recent studies indicated also crucial immune microenvironment regulation, stemness maintenance, therapeutic resistance, phenotype shaping, etc. Wnt/β-Catenin, Wnt–planar cell polarity (PCP), Wnt–Ca 2+ are three well-established pathways share overlapping components play different roles In this review, we summarize main findings concerning relationship between provide an overview existing mechanisms, challenges, potential opportunities for advancing therapy diagnosis cancer.

Language: Английский

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