Neuron,
Journal Year:
2023,
Volume and Issue:
111(23), P. 3745 - 3764.e7
Published: Sept. 29, 2023
Leptomeninges,
consisting
of
the
pia
mater
and
arachnoid,
form
a
connective
tissue
investment
barrier
enclosure
brain.
The
exact
nature
leptomeningeal
cells
has
long
been
debated.
In
this
study,
we
identify
five
molecularly
distinct
fibroblast-like
transcriptomes
in
cerebral
leptomeninges;
link
them
to
anatomically
cell
types
pia,
inner
outer
arachnoid
barrier,
dural
border
layer;
contrast
sixth
transcriptome
present
choroid
plexus
median
eminence.
Newly
identified
transcriptional
markers
enabled
molecular
characterization
responsible
for
adherence
layers
one
another
barrier.
These
also
proved
useful
identifying
features
development,
injury,
repair
that
were
preserved
or
changed
after
traumatic
brain
injury.
Together,
findings
highlight
value
fibroblast
subsets
their
cellular
locations
toward
advancing
understanding
physiology
pathology.
Science,
Journal Year:
2022,
Volume and Issue:
377(6606)
Published: Aug. 4, 2022
Drosophila
melanogaster
is
a
powerful,
long-standing
model
for
metazoan
development
and
gene
regulation.
We
profiled
chromatin
accessibility
in
almost
1
million
expression
half
nuclei
from
overlapping
windows
spanning
the
entirety
of
embryogenesis.
Leveraging
developmental
asynchronicity
within
embryo
collections,
we
applied
deep
neural
networks
to
infer
age
each
nucleus,
resulting
continuous,
multimodal
views
molecular
cellular
transitions
absolute
time.
identify
cell
lineages;
their
relationships;
link
dynamic
changes
enhancer
usage,
transcription
factor
(TF)
expression,
TFs’
cognate
motifs.
With
these
data,
dynamics
usage
can
be
explored
across
lineages
at
scale
minutes,
including
precise
like
zygotic
genome
activation.
Advanced Materials,
Journal Year:
2023,
Volume and Issue:
35(38)
Published: June 26, 2023
Abstract
The
treatment
of
reperfusion
injury
after
ischemic
stroke
remains
unsatisfactory
since
the
blood‐brain
barrier
(BBB)
prevents
most
neuroprotective
agents
from
entering
brain.
Here,
a
strategy
is
proposed
based
on
bacteria‐derived
outer‐membrane
vesicle
(OMV)
hitchhiking
neutrophils
for
enhanced
brain
delivery
pioglitazone
(PGZ)
to
treat
stroke.
By
encapsulating
PGZ
into
OMV,
resulting
OMV@PGZ
nanoparticles
inherit
functions
associated
with
bacterial
outer
membrane,
making
them
ideal
decoys
neutrophil
uptake.
results
show
that
simultaneously
inhibits
activation
nucleotide
oligomerization‐like
receptor
protein
3
(NLRP3)
inflammasomes
and
ferroptosis
reduces
exert
effect.
Notably,
transcription
factors
Pou2f1
Nrf1
oligodendrocytes
are
identified
first
time
be
involved
in
this
process
promoted
neural
repair
by
single‐nucleus
RNA
sequencing
(snRNA‐seq).
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: March 6, 2023
The
mammalian
brain
is
composed
of
millions
to
billions
cells
that
are
organized
into
numerous
cell
types
with
specific
spatial
distribution
patterns
and
structural
functional
properties.
An
essential
step
towards
understanding
function
obtain
a
parts
list,
i.e.,
catalog
types,
the
brain.
Here,
we
report
comprehensive
high-resolution
transcriptomic
type
atlas
for
whole
adult
mouse
was
created
based
on
combination
two
single-cell-level,
whole-brain-scale
datasets:
single-cell
RNA-sequencing
(scRNA-seq)
dataset
~7
million
profiled,
spatially
resolved
~4.3
using
MERFISH.
hierarchically
five
nested
levels
classification:
7
divisions,
32
classes,
306
subclasses,
1,045
supertypes
5,200
clusters.
We
systematically
analyzed
neuronal,
non-neuronal,
immature
neuronal
across
identified
high
degree
correspondence
between
identity
specificity
each
type.
results
reveal
unique
features
organization
in
different
regions,
particular,
dichotomy
dorsal
ventral
brain:
part
contains
relatively
fewer
yet
highly
divergent
whereas
more
closely
related
other.
also
characterized
cell-type
expression
neurotransmitters,
neuropeptides,
transcription
factors.
study
uncovered
extraordinary
diversity
heterogeneity
neurotransmitter
neuropeptide
co-expression
brain,
suggesting
they
mediate
myriad
modes
intercellular
communications.
Finally,
found
factors
major
determinants
classification
combinatorial
factor
code
defines
all
whole-mouse-brain
establishes
benchmark
reference
foundational
resource
deep
integrative
investigations
circuit
function,
development,
evolution
Science,
Journal Year:
2023,
Volume and Issue:
380(6650)
Published: June 15, 2023
Aging
is
characterized
by
a
decline
in
tissue
function,
but
the
underlying
changes
at
cellular
resolution
across
organism
remain
unclear.
Here,
we
present
Fly
Cell
Atlas,
single-nucleus
transcriptomic
map
of
whole
aging
Neuron,
Journal Year:
2023,
Volume and Issue:
111(23), P. 3745 - 3764.e7
Published: Sept. 29, 2023
Leptomeninges,
consisting
of
the
pia
mater
and
arachnoid,
form
a
connective
tissue
investment
barrier
enclosure
brain.
The
exact
nature
leptomeningeal
cells
has
long
been
debated.
In
this
study,
we
identify
five
molecularly
distinct
fibroblast-like
transcriptomes
in
cerebral
leptomeninges;
link
them
to
anatomically
cell
types
pia,
inner
outer
arachnoid
barrier,
dural
border
layer;
contrast
sixth
transcriptome
present
choroid
plexus
median
eminence.
Newly
identified
transcriptional
markers
enabled
molecular
characterization
responsible
for
adherence
layers
one
another
barrier.
These
also
proved
useful
identifying
features
development,
injury,
repair
that
were
preserved
or
changed
after
traumatic
brain
injury.
Together,
findings
highlight
value
fibroblast
subsets
their
cellular
locations
toward
advancing
understanding
physiology
pathology.
