Reviewer #1 (Public Review): A split-GAL4 driver line resource for Drosophila CNS cell types DOI Open Access

Published: July 30, 2024

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. In addition stocks for well-characterized lines, we make available 300,000 new 3D lines.

Language: Английский

A split-GAL4 driver line resource forDrosophilaneuron types DOI Creative Commons
Geoffrey W. Meissner,

Allison Vannan,

Jennifer Jeter

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 10, 2024

Abstract Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. Previously published new included, all validated for expression curated optimal type specificity across diverse types. In addition stocks well-characterized lines, we make available 300,000 3D lines.

Language: Английский

Citations

12
A split-GAL4 driver line resource for Drosophila neuron types DOI Creative Commons
Geoffrey W. Meissner,

Allison Vannan,

Jennifer Jeter

et al.

eLife, Journal Year: 2025, Volume and Issue: 13

Published: Jan. 24, 2025

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system (CNS) have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult CNS 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. Previously published new included, all validated for expression curated optimal cell-type specificity across diverse types. In addition stocks well-characterized lines, we make available 300,000 3D lines.

Language: Английский

Citations

1
Selective integration of diverse taste inputs within a single taste modality DOI Creative Commons

Julia U. Deere,

Arvin A Sarkissian,

Meifeng Yang

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Jan. 24, 2023

A fundamental question in sensory processing is how different channels of input are processed to regulate behavior. Different may converge onto common downstream pathways drive the same behaviors, or they activate separate distinct behaviors. We investigated this Drosophila bitter taste system, which contains diverse bitter-sensing cells residing organs. First, we optogenetically activated subsets neurons within each organ. These elicited broad and highly overlapping behavioral effects, suggesting that pathways, but also observed differences argue for biased convergence. Consistent with these results, transsynaptic tracing revealed organs connect connectivity. one type neuron projects higher brain. integrate from multiple specific taste-related then traced circuits, providing first glimpse into Together, results reveal inputs selectively integrated early circuit, enabling pooling information, while circuit diverges have roles.

Language: Английский

Citations

17
Increased sugar valuation contributes to the evolutionary shift in egg-laying behavior of the fruit pest Drosophila suzukii DOI Creative Commons
Matthieu Cavey, Bernard Charroux,

Solène Travaillard

et al.

PLoS Biology, Journal Year: 2023, Volume and Issue: 21(12), P. e3002432 - e3002432

Published: Dec. 11, 2023

Behavior evolution can promote the emergence of agricultural pests by changing their ecological niche. For example, insect pest Drosophila suzukii has shifted its oviposition (egg-laying) niche from fermented fruits to ripe, non-fermented fruits, causing significant damage a wide range fruit crops worldwide. We investigate chemosensory changes underlying this evolutionary shift and ask whether sugars, which are depleted during fermentation, important gustatory cues that direct D. sweet, ripe fruits. show expanded responses lower sugar concentrations than model melanogaster, prefers lay eggs on fruit. The increased response correlates with an increase in value relative strawberry substrate decisions. In addition, we genetic manipulation sugar-gustatory receptor neurons (GRNs) perception is required for prefer over substrate, but not melanogaster substrate. Thus, major determinant suzukii's choice complex substrates. Calcium imaging experiments brain's primary center (suboesophageal zone) GRNs more sensitive counterparts, suggesting valuation encoded downstream circuits central nervous system (CNS). Taken together, our data suggest brain computations involved driving preference

Language: Английский

Citations

12
Neuronal substrates of egg-laying behaviour at the abdominal ganglion of Drosophila melanogaster DOI Creative Commons

Cristina Oliveira-Ferreira,

Miguel Gaspar, Maria Luísa Vasconcelos

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Dec. 11, 2023

Egg-laying in Drosophila is the product of post-mating physiological and behavioural changes that culminate a stereotyped sequence actions. harbours great potential as paradigm to uncover how appropriate motor circuits are organized activated generate behaviour. To study this programme, we first describe different phases egg-laying programme specific actions associated with each phase. Using combination neuronal activation silencing experiments, identify neurons (OvAbg) abdominal ganglion key players egg-laying. functionally characterise subsets OvAbg, used an intersectional approach neurotransmitter lines-VGlut, Cha Gad1. We show OvAbg/VGlut promote initiation egg deposition mating status dependent way. OvAbg/Cha required exploration phases, though leads specifically expulsion. Experiments OvAbg/Gad1 they participate deposition. further functional connection OvAbg brain neurons. This provides insight into organization underlying complex

Language: Английский

Citations

12
eLife assessment: A split-GAL4 driver line resource for Drosophila CNS cell types DOI Open Access
Tânia Reis

Published: July 30, 2024

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. In addition stocks for well-characterized lines, we make available 300,000 new 3D lines.

Language: Английский

Citations

4
A split-GAL4 driver line resource for Drosophila neuron types DOI Open Access
Geoffrey W. Meissner,

Allison Vannan,

Jennifer Jeter

et al.

Published: Jan. 6, 2025

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. Previously published new included, all validated for expression curated optimal type specificity across diverse types. In addition stocks well-characterized lines, we make available 300,000 3D lines.

Language: Английский

Citations

0
A neuronal substrate for translating nutrient state and resource density estimations into foraging decisions DOI Creative Commons
Dennis Goldschmidt, Ibrahim Tastekin, Daniel Münch

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 19, 2023

Foraging animals must balance the costs of exploring their surroundings with potential benefits finding nutritional resources. Each time an animal encounters a food source it decide whether to initiate feeding or continue searching for potentially better options. Experimental evidence and patch foraging models predict that this decision depends on both state density available resources in environment. How brain integrates such internal external states adapt so-called exploration-exploitation trade-off remains poorly understood. We use video-based tracking show Drosophila regulates engage patches based travel between patches, latter being measure To uncover neuronal basis process, we performed neurogenetic silencing screen more than 400 genetic driver lines sparse expression patterns fly brain. identified population neurons central complex acts as key regulator patch. manipulating activity these alters probability engage, is modulated by protein animal, perturbs ability adjust decisions fly’s patches. Taken together, our results reveal substrate information control specific decision, therefore provide important step towards mechanistic explanation cognitive computations resolve cost-benefit trade-offs.

Language: Английский

Citations

10
Descending control of motor sequences in DOI Creative Commons
J. Simpson

Current Opinion in Neurobiology, Journal Year: 2023, Volume and Issue: 84, P. 102822 - 102822

Published: Dec. 13, 2023

The descending neurons connecting the fly's brain to its ventral nerve cord respond sensory stimuli and evoke motor programs of varying complexity. Anatomical characterization their synaptic connections suggests how these circuits organize movements, while optogenetic manipulation activity reveals what behaviors they can induce. Monitoring responses or during behavior performance indicates information may encode. Recent advances in all three approaches make an excellent place better understand sensorimotor integration transformation required for nervous systems govern sequences that constitute animal behavior.

Language: Английский

Citations

10
A split-GAL4 driver line resource for Drosophila CNS cell types DOI Open Access
Geoffrey W. Meissner,

Allison Vannan,

Jennifer Jeter

et al.

Published: July 30, 2024

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. In addition stocks for well-characterized lines, we make available 300,000 new 3D lines.

Language: Английский

Citations

3