Methods in molecular biology, Journal Year: 2024, Volume and Issue: unknown, P. 129 - 152
Published: Jan. 1, 2024
Language: Английский
Cell, Journal Year: 2024, Volume and Issue: 187(17), P. 4488 - 4519
Published: Aug. 1, 2024
Language: Английский
Citations
24
Nature, Journal Year: 2024, Volume and Issue: 630(8017), P. 596 - 608
Published: June 19, 2024
Language: Английский
Citations
18
Cell, Journal Year: 2024, Volume and Issue: 187(21), P. 5838 - 5857
Published: Oct. 1, 2024
Language: Английский
Citations
11
Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)
Published: March 26, 2025
Chromatin topology can impact gene regulation, but how evolutionary divergence in chromatin has shaped regulatory landscapes for distinctive human traits remains poorly understood. CTCF sites determine by forming domains and loops. Here, we show CTCF-mediated at the domain loop scales during primate evolution, elucidating distinct mechanisms shaping landscapes. Human-specific divergent lead to a broad rewiring of transcriptional Divergent loops concord with species-specific enhancer activity, influencing connectivity target genes concordant yet constrained manner. Under this mechanism, establish role human-specific isoform diversity, functional implications disease susceptibility. Furthermore, validate function these using forebrain organoids. This study advances our understanding genetic evolution from perspective genome architecture. regulation. Wu et al. that changes structure, particularly loops, drive diversity contributing traits.
Language: Английский
Citations
1
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: April 23, 2024
Thyroid hormones (TH) play critical roles during nervous system development and patients carrying coding variants of MCT8 (monocarboxylate transporter 8) or THRA (thyroid hormone receptor alpha) present a spectrum neurological phenotypes resulting from perturbed local TH action early brain development. Recently, human cerebral organoids (hCOs) emerged as powerful in vitro tools for disease modelling recapitulating key aspects cortex To begin exploring prospects this model thyroid research, we performed detailed characterization the spatiotemporal expression developing hCOs. Immunostaining showed membrane neuronal progenitor cell types including neuroepithelial cells, radial glia cells (RGCs), intermediate progenitors outer RGCs. In addition, detected robust protein deep layer upper neurons. Spatiotemporal SLC16A2 mRNA expression, by fluorescent situ hybridization (FISH), was highly concordant with across cortical layers. FISH already neuroepithelium before onset neurogenesis. remained low ventricular zone, increased subventricular zone whereas strong observed excitatory combination up-regulation known T3 response genes following treatment, these observations show that hCOs provide promising experimentally tractable to probe neurogenesis eventually consequences impaired function
Language: Английский
Citations
7
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 15, 2024
The disproportionate expansion of telencephalic structures during human evolution involved tradeoffs that imposed greater connectivity and metabolic demands on midbrain dopaminergic neurons. Despite the central role neurons in human-enriched disorders, molecular specializations associated with human-specific features vulnerabilities system remain unexplored. Here, we establish a phylogeny-in-a-dish approach to examine gene regulatory by differentiating pools human, chimpanzee, orangutan, macaque pluripotent stem cells into ventral organoids capable forming long-range projections, spontaneous activity, dopamine release. We identify expression changes related axonal transport mitochondria reactive oxygen species buffering candidate
Language: Английский
Citations
4
Nature Neuroscience, Journal Year: 2024, Volume and Issue: 27(12), P. 2310 - 2325
Published: Dec. 1, 2024
Language: Английский
Citations
4
Medicina, Journal Year: 2024, Volume and Issue: 60(12), P. 2085 - 2085
Published: Dec. 19, 2024
Background and Objectives: This systematic review examines the integration of gamified health promotion strategies in school settings, with a focus on their potential to positively influence behaviors promote well-being among adolescents. study explores incorporation cognitive behavioral therapy (CBT), artificial intelligence, neuropsychological principles interventions, aiming enhance engagement effectiveness. Materials Methods: A narrative synthesis 56 studies, following PRISMA guidelines, underscores significant impact these interventions mental outcomes, emphasizing reductions anxiety, depression, burnout while improving coping skills lifestyle habits. The key areas emotional regulation, flexibility, adherence mechanisms is explored through quantitative qualitative syntheses underscore intervention effectiveness design principles. Results: highlights high-quality evidence supporting use gamification educational settings calls for further research optimize elements address implementation barriers. findings propose that well-designed can effectively engage students, healthy behaviors, improve acknowledging need studies explore underlying long-term effects. Conclusions: Gamified embed CBT are promising promoting schoolchildren. Although indicates they effective psychological needed features overcome challenges ensure wider more sustainable application.
Language: Английский
Citations
4
Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(3)
Published: Jan. 13, 2025
Human brain evolution is marked by a disproportionate expansion of cortical regions associated with advanced perceptual and cognitive functions. While this often attributed to the emergence novel specialized areas, modifications evolutionarily conserved also have been linked species-specific behaviors. Distinguishing between these two evolutionary outcomes has limited ability make direct comparisons species. Here, we addressed limitation examining human visual cortex relative macaques using common functional architecture: retinotopy. Our findings revealed that primarily driven increases in surface area map architecture present rather than an increase number individual areas. This was not uniform, higher-order particularly parietal cortex, exhibiting largest growth. Comparisons neonate adult humans areal size differences were already established at birth. A meta-analysis neuroimaging studies indicated most expanded areas are functions beyond processing. These results suggest adaptations may be rooted architecture, even sensory contributing broader characteristic behavior.
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 23, 2025
The superior colliculus (SC), a midbrain sensorimotor hub, is anatomically and functionally similar across vertebrates, but how its cell types have evolved unclear. Using single-nucleus transcriptomics, we compared the SC's molecular cellular organization in mice, tree shrews, humans. Despite over 96 million years of evolutionary divergence, identified ∼30 consensus neuronal subtypes, including Cbln2 + neurons that form SC-pulvinar circuit mice shrews. Synapse-related genes were among most conserved, unlike neocortex, suggesting co-conservation synaptic circuitry. In contrast, cilia-related diverged significantly species, highlighting potential importance primary cilium SC evolution. Additionally, novel inhibitory neuron shrews humans not mice. Our findings reveal has by conserving genes, circuitry, while diversifying ciliary gene expression an subtype.
Language: Английский
Citations
0