Intranasal vaccination with an NDV-vectored SARS-CoV-2 vaccine protects against Delta and Omicron challenges DOI Creative Commons
Bryce M. Warner,

Jacob G. E. Yates,

Robert Vendramelli

et al.

npj Vaccines, Journal Year: 2024, Volume and Issue: 9(1)

Published: May 23, 2024

Abstract The rapid development and deployment of vaccines following the emergence severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been estimated to have saved millions lives. Despite their immense success, there remains a need for next-generation vaccination approaches SARS-CoV-2 future emerging coronaviruses other viruses. Here we utilized Newcastle Disease virus (NDV) vectored vaccine expressing ancestral spike protein in pre-fusion stabilized chimeric conformation (NDV-PFS). When delivered intranasally, NDV-PFS protected both Syrian hamsters K18 mice against Delta Omicron variants concern. Additionally, intranasal induced robust, durable protection that was extended 6 months post-vaccination. Overall, our data provide evidence NDV-vectored represent viable mucosal approach.

Language: Английский

Virological and antigenic characteristics of SARS-CoV-2 variants LF.7.2.1, NP.1, and LP.8.1 DOI
Jingyi Liu, Yuanling Yu, Sijie Yang

et al.

The Lancet Infectious Diseases, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

1
Long-Term Immune Consequences of Initial SARS-CoV-2 A.23.1 Exposure: A Longitudinal Study of Antibody Responses and Cross-Neutralization in a Ugandan Cohort DOI Creative Commons

Gerald Kevin Oluka,

Jackson Sembera,

Joseph Ssebwana Katende

et al.

Vaccines, Journal Year: 2025, Volume and Issue: 13(2), P. 143 - 143

Published: Jan. 29, 2025

Background: This study assessed the long-term dynamics of neutralizing antibodies in a Ugandan cohort primarily exposed to A.23.1 SARS-CoV-2 variant, examining how this shaped immune breadth and potency against diverse strains following infection prototype-based vaccination. Methods: We conducted 427-day retrospective analysis 41 participants across multiple waves, assessing binding antibody responses using in-house ELISA pseudotyped virus neutralization assays. quantified key variants, A.23.1, D614G, Delta, BA.4, capturing evolving immunity pandemic. Results: Neutralizing titers remained significantly higher than those highlighting solid memory infection. Consistently lower were observed for BA.4 all time points, aligning with its strong immune-evasion capability. Correlations between spike-directed IgG (S-IgG) concentrations stronger no correlation BA.4. ChAdOx1-S vaccination substantially elevated most notably essential role boosting immunity, even individuals initially low titers. Conclusions: Initial exposure variant triggered potent responses, shaping during subsequent exposures. These findings highlight importance accounting early viral exposures vaccine development public health planning. The distinctly response highlights need continuous antigenic monitoring timely updates protection emerging variants. Vaccination remains reinforcing sustaining

Language: Английский

Citations

1
现代疫苗学赋能新突发病毒性传染病疫苗的快速“智造”——以猴痘疫情为例 DOI
Tingting Zheng, Han Wang, Qihui Wang

et al.

Chinese Science Bulletin (Chinese Version), Journal Year: 2025, Volume and Issue: 70(7), P. 789 - 798

Published: Feb. 11, 2025

Citations

1
Full-spike deep mutational scanning helps predict the evolutionary success of SARS-CoV-2 clades DOI Creative Commons
Bernadeta Dadonaite,

Jack Brown,

Teagan McMahon

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 14, 2023

SARS-CoV-2 variants acquire mutations in spike that promote immune evasion and impact other properties contribute to viral fitness such as ACE2 receptor binding cell entry. Knowledge of how affect these phenotypes can provide insight into the current potential future evolution virus. Here we use pseudovirus deep mutational scanning measure >9,000 across full XBB.1.5 BA.2 spikes binding, entry, or escape from human sera. We find outside receptor-binding domain (RBD) have meaningfully impacted during evolution. also neutralization by serum individuals who recently had infections. The strongest are RBD at sites 357, 420, 440, 456, 473-however, antigenic impacts vary individuals. identify strong RBD; however many them decrease suggesting they act modulating conformation. Notably, growth rates clades be explained substantial part measured effects on phenotypes, our data could enable better prediction

Language: Английский

Citations

21
Intranasal vaccination with an NDV-vectored SARS-CoV-2 vaccine protects against Delta and Omicron challenges DOI Creative Commons
Bryce M. Warner,

Jacob G. E. Yates,

Robert Vendramelli

et al.

npj Vaccines, Journal Year: 2024, Volume and Issue: 9(1)

Published: May 23, 2024

Abstract The rapid development and deployment of vaccines following the emergence severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been estimated to have saved millions lives. Despite their immense success, there remains a need for next-generation vaccination approaches SARS-CoV-2 future emerging coronaviruses other viruses. Here we utilized Newcastle Disease virus (NDV) vectored vaccine expressing ancestral spike protein in pre-fusion stabilized chimeric conformation (NDV-PFS). When delivered intranasally, NDV-PFS protected both Syrian hamsters K18 mice against Delta Omicron variants concern. Additionally, intranasal induced robust, durable protection that was extended 6 months post-vaccination. Overall, our data provide evidence NDV-vectored represent viable mucosal approach.

Language: Английский

Citations

7