Maize TIR-only Proteins ZmTIR1 and ZmTIR2, but not ZmTIR3 Confer Auto-active Hypersensitive Response Likely by Forming Condensation DOI Creative Commons
Zhen Kang, Qidong Ge,

M. Zhang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

SUMMARY Nucleotide binding, leucine-rich-repeat (NLR) proteins are the major intracellular receptors for defending against pathogen infection. The recognition between NLRs and secreted effectors often triggers a localized programmed cell death termed hypersensitive response (HR). Despite significant progresses have been achieved in understanding canonical with N-terminal Toll/interleukin-1 receptor (TIR) domains, molecular mechanisms underlying TIR-only plant immune responses remain unclear. In this study, we identified six TIR-containing maize, including three proteins. Functional analysis showed that ZmTIR1 ZmTIR2, but not ZmTIR3, confer autoactive HR when transiently expressed N. benthamiana . autoactivity conferred by ZmTIR2 depends on EDS1-PAD4-RNL module their putative NADase activities. Interestingly, predominantly localize punctate dots likely form condensation, while ZmTIR3 mainly localizes cytoplasm nucleus. Two specific amino acids BB-loop region were to be required ZmTIR1- ZmTIR2-mediated condensation formation auto-HR. Furthermore, ZmTIR induced Cochliobolus heterostrophus , causal agent of southern leaf blight (SLB) knock-down expression or decreased resistance SLB maize. Our study reveals novel mechanism monocot maize responses.

Language: Английский

Phase separation activates plant TIR-only immune receptors DOI
Li Wan

Science Bulletin, Journal Year: 2024, Volume and Issue: unknown

Published: May 1, 2024

Language: Английский

Citations

1
TIR domain protein-mediated phase separation activates plant immunity DOI Creative Commons
Hadiatullah Hadiatullah, Lei Li, Zhiyuan Hao

et al.

Advanced Agrochem, Journal Year: 2024, Volume and Issue: 3(3), P. 183 - 184

Published: May 1, 2024

Language: Английский

Citations

0
Bacillus Velezensis Yn2111 Promotes Plant Growth, But Exacerbates Fusarium Wilt of Banana by Blocking the Expression of Cavendish Banana Amine Oxidase Muao DOI

Hongwei Yu,

Guangdong Zhou, Wenlong Zhang

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Cryo-EM Structure of an Active Bacterial SIR2-STAND Filament DOI Creative Commons
Yiqun Wang, Yuqing Tian, Yang Xu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 16, 2024

Abstract The signal transduction ATPases with numerous domains (STAND) superfamily encompasses widely distributed immune systems across bacteria, eukaryotes and archaea. bacterial antiviral STAND type 5 (Avs5) contains an N-terminal Sirtuin (SIR2) domain, which protects against phage invasion. Despite the established roles of SIR2 in prokaryotic eukaryotic immunity, mechanism underlying their collaboration remains unclear. Here we present cryo-EM structures Escherichia fergusonii Avs5 ( Ef Avs5) filaments, elucidating mechanisms dimerization, filamentation, filament clustering, ATP binding NAD + hydrolysis, all are crucial for anti-phage defense. nucleotide-binding oligomerization (NOD) engage intra- inter-dimer interaction to form individual filament, while outward C-terminal contribute bundle formation. Filamentation potentially stabilizes dimeric configuration, thereby activating NADase activity Avs5. is deficient ATPase activity, but elevated concentrations can impede its activity. Together, uncover assembly as a unique switch enzyme activities perform antiphage defenses. Keypoints depletes assembles bundled filaments that hydrolyze . domain NOD collaborate filament. building block dimer. complex regulated by ATP.

Language: Английский

Citations

0
Filamentation activates bacterial NLR-like antiviral protein DOI Creative Commons
Jianting Zheng, Yiqun Wang, Yuqing Tian

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 15, 2024

Abstract Bacterial antiviral STANDs (Avs) are evolutionarily related to the nucleotide-binding leucine-rich repeat containing receptors (NLRs) widely distributed in immune systems across animals and plants. EfAvs5, an Avs type 5 protein from Escherichia fergusonii, contains N-terminal SIR2 effector domain, a oligomerization domain (NOD) C-terminal sensor conferring protection against diverse phage invasions. Despite established roles of STAND prokaryotic eukaryotic immunity, mechanism underlying their collaboration remains unclear. Here we present cryo-EM structures EfAvs5 filaments, elucidating mechanisms dimerization, filamentation, filament clustering, ATP binding NAD+ hydrolysis, all which crucial for anti-phage defense. The domains NODs engage intra- inter-dimer interaction form individual filament, while outward contribute bundle formation. Filamentation potentially stabilizes dimeric configuration, thereby activating NADase activity EfAvs5. is deficient ATPase activity, but elevated concentrations can impede its activity. Together, uncover assembly Avs5 as unique switch enzyme activities perform defenses, emphasizing conserved role filamentation signaling life forms.

Language: Английский

Citations

0
PP2 gene family in Phyllostachys edulis: identification, characterization, and expression profiles DOI Creative Commons
Liumeng Zheng, Huifang Zheng, Xianzhe Zheng

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Nov. 13, 2024

Phloem protein 2 (PP2), a dimeric lectin, is known for its involvement in plant responses to biotic and abiotic stresses. However, research on PP2 proteins Moso bamboo lacking. In this study, comprehensive genome-wide analysis of the PP2-like gene family was conducted (Phyllostachys edulis), which has significant economic ecological value. Using HMMER3 search InterPro domain analysis, 23 genes (PhePP2-1 PhePP2-23) were identified P. edulis genome. These distributed across 12 chromosomal scaffolds, with ranging from 216 556 amino acids length. Phylogenetic including 163 eight species, revealed six distinct groups, Group III V being largest. Gene structure motif analyses indicated conserved domains PhePP2 proteins. addition, Cis-element promoter regions highlighted their potential regulatory roles hormone, stress, light responses. Expression pattern using RNA-seq data showed differential expression under drought, salt, salicylic acid, abscisic acid treatments, indicating stress response pathways. Furthermore, qPCR validation different tissues organs confirmed profiles selected genes. This study provides understanding functional insights into applications enhancing tolerance growth plants.

Language: Английский

Citations

0
miR158a negatively regulates plant resistance to Phytophthora parasitica by repressing AtTN7 that requires EDS1‐PAD4‐ADR1 complex in Arabidopsis thaliana DOI
Yilin Li,

Xiuhong Gou,

R. Ma

et al.

The Plant Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 5, 2024

SUMMARY Small RNAs are involved in diverse cellular processes, including plant immunity to pathogens. Here, we report that miR158a negatively regulates the oomycete pathogen Phytophthora parasitica Arabidopsis thaliana . By performing real‐time quantitative PCR, transient expression, and RNA ligase‐mediated 5′ rapid amplification of cDNA ends assays, demonstrate downregulates AtTN7 expression by cleaving its 3′‐untranslated region. positively affects encodes a truncated intracellular nucleotide‐binding site leucine‐rich repeat receptor containing Toll/interleukin‐1 receptor. can degrade oxidized forms nicotinamide adenine dinucleotide (NAD+). Further genetic molecular analyses reveal Enhanced Disease Susceptibility 1‐Phytoalexin Deficient 4‐Activated Resistance 1 complex is required for AtTN7‐mediated immunity. ADR1‐dependent Ca 2+ influx crucial activating salicylic acid signaling condition AtTN7‐triggered Our study uncovers immune roles regulatory mechanisms target Both miR158a‐downregulation ‐overexpression lead enhanced resistance P. without affecting growth phenotypes, suggesting their application potentials utilization miRNAs identifying novel genes development germplasm resources with disease resistance.

Language: Английский

Citations

0
Leucine-Rich Repeat Protein Family Regulates Stress Tolerance and Development in Plants DOI Creative Commons

Hao Zhiqi,

Wang Tingyi,

Dongdong Chen

et al.

Rice Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

Citations

0
Maize TIR-only Proteins ZmTIR1 and ZmTIR2, but not ZmTIR3 Confer Auto-active Hypersensitive Response Likely by Forming Condensation DOI Creative Commons
Zhen Kang, Qidong Ge,

M. Zhang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

SUMMARY Nucleotide binding, leucine-rich-repeat (NLR) proteins are the major intracellular receptors for defending against pathogen infection. The recognition between NLRs and secreted effectors often triggers a localized programmed cell death termed hypersensitive response (HR). Despite significant progresses have been achieved in understanding canonical with N-terminal Toll/interleukin-1 receptor (TIR) domains, molecular mechanisms underlying TIR-only plant immune responses remain unclear. In this study, we identified six TIR-containing maize, including three proteins. Functional analysis showed that ZmTIR1 ZmTIR2, but not ZmTIR3, confer autoactive HR when transiently expressed N. benthamiana . autoactivity conferred by ZmTIR2 depends on EDS1-PAD4-RNL module their putative NADase activities. Interestingly, predominantly localize punctate dots likely form condensation, while ZmTIR3 mainly localizes cytoplasm nucleus. Two specific amino acids BB-loop region were to be required ZmTIR1- ZmTIR2-mediated condensation formation auto-HR. Furthermore, ZmTIR induced Cochliobolus heterostrophus , causal agent of southern leaf blight (SLB) knock-down expression or decreased resistance SLB maize. Our study reveals novel mechanism monocot maize responses.

Language: Английский

Citations

0