Molecules,
Journal Year:
2024,
Volume and Issue:
29(23), P. 5772 - 5772
Published: Dec. 6, 2024
Enzyme
catalysis
was
traditionally
used
by
various
human
cultures
to
create
value
long
before
its
basic
concepts
were
uncovered.
This
achieved
transforming
the
raw
materials
available
from
natural
resources
into
useful
products.
Tremendous
scientific
and
technological
progress
has
been
made
globally
in
understanding
what
constitutes
an
enzyme;
reactions
enzymes
can
catalyze;
how
search,
develop,
apply,
improve
make
desired
The
properties
of
as
nature's
preferred
catalysts,
such
their
high
selectivity,
diversity,
adaptability,
enable
optimal
function,
whether
single
or
multiple
reactions.
Excellent
opportunities
for
resource-efficient
manufacturing
compounds
are
provided
actions
working
reaction
cascades
pathways
within
same
space,
like
molecular
robots
along
a
production
line.
plays
increasingly
prominent
role
industrial
innovation
responsible
areas,
green
sustainable
chemistry
white
biotechnology.
Sources
inspiration
include
current
supply
chain
challenges,
treasure
enzymes,
engineer
tailor-made
enzymes.
Making
best
use
power
enzyme
is
essential
changing
products
manufactured;
renewable
biobased
replace
fossil-based
resources;
improving
safety,
health,
environmental
aspects
processes
support
cleaner
more
production.
Catalysts,
Journal Year:
2025,
Volume and Issue:
15(1), P. 37 - 37
Published: Jan. 3, 2025
Nicotinamide
mononucleotide
(NMN)
has
emerged
as
a
promising
non-natural
cofactor
with
significant
potential
to
transform
biocatalysis,
synthetic
biology,
and
therapeutic
applications.
By
modulating
NAD⁺
metabolism,
NMN
offers
unique
advantages
in
enzymatic
reactions,
metabolic
engineering,
regenerative
medicine.
This
review
provides
comprehensive
analysis
of
NMN’s
biochemical
properties,
mechanisms
action,
diverse
Emphasis
is
placed
on
its
role
addressing
challenges
multi-enzyme
cascades,
biofuel
production,
the
synthesis
high-value
chemicals.
The
paper
also
highlights
critical
research
gaps,
including
need
for
scalable
methods,
improved
integration
into
systems,
toxicity
studies
use.
Emerging
technologies
such
AI-driven
enzyme
design
CRISPR-based
genome
engineering
are
discussed
transformative
tools
optimizing
NMN-dependent
pathways.
Furthermore,
synergistic
biology
innovations,
cell-free
systems
dynamic
regulatory
networks,
explored,
paving
way
precise
modular
biotechnological
solutions.
Looking
forward,
versatility
positions
it
pivotal
tool
advancing
sustainable
bioprocessing
precision
Addressing
current
limitations
through
interdisciplinary
approaches
will
enable
redefine
boundaries
innovation.
serves
roadmap
leveraging
across
scientific
industrial
domains.
Research Square (Research Square),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 17, 2025
Abstract
Enzymatic
reductions
catalyzed
by
reductases,
such
as
aldo/ketoreductases
(AKRs),
imine
and
ene
reductases
generally
depend
on
the
regeneration
of
nicotinamide
cofactors
for
industrial
viability.
This
usually
involves
addition
a
cosubstrate
second
enzyme,
dehydrogenase,
e.g.
glucose
/
dehydrogenase.
For
commercial
viability
it
would
be
more
interesting
to
use
water
sacrificial
supply
necessary
hydrogen
atoms.
is
possible
using
photocatalytic
methods
involving
precious
metal,
Rh
Ru,
complexes
electron
mediators.
A
problem
associated
with
photobiocatalysis
in
general
weak
penetration
ultraviolet
or
visible
light
biological
systems.
Here
we
report
unprecedented
near
infrared
(NIR)
combination
hybrid
photoenzyme
based
IR
responsive
reductive
graphene
quantum
dots
(rGQDs)
that
are
non-toxic
AKRs.
We
envisaged
immobilization
AKR
close
proximity
rGQDs
could
enable
direct
transfer
atoms
from
prochiral
ketone
substrate
without
requiring
cofactor.
To
test
this
hypothesis,
immobilized
surface
cross-linked
AKR.
The
resulting
rGQD/AKR
photobiocatalyst
mediated
synthesis
pharmaceutical
intermediate,
(R)-1-[3,5-bis(trifluoromethyl)-phenyl]
ethanol
((R)-3,5-BTPE)
corresponding
ketone,
82%
yield
>
99.99%
ee
under
50
mW·cm−
2
illumination.
work
opens
new
avenues
create
artificial
photoenzymes
simplify
in
vitro
biocatalysis
coupling
renewable
solar
energy
sustainable
chemical
production.
Since
accounts
about
half
entire
spectrum
its
efficient
utilization
crucial
applications
sunlight.
have
demonstrated
our
can
effectively
utilize
catalyze
enzymatic
reduction
ketone.
these
photobiocatalysts
insoluble
solids
they
readily
recovered,
recycled
potentially
employed
continuous
flow
operation.
Natural Product Reports,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
In
this
review
we
highlight
recent
advancements
in
mass
spectrometry
that
have
allowed
for
more
efficient,
robust,
and
rigorous
enzyme
engineering
various
applications
relating
to
natural
products
chemistry.
European Journal of Organic Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 11, 2025
Abstract
Amino
acids
play
significant
roles
in
the
study
of
life
sciences,
pharmaceutical
R&D,
bioengineering,
and
so
on.
Artificial
amino
acid‐based
drugs
are
important
advancing
therapeutic
innovation
for
multiple
diseases
including
chronic
pain,
diabetes,
Alzheimer's
disease.
On
other
hand,
multicomponent
reactions
(MCRs)
demonstrated
to
be
a
powerful
strategy
construction
complicated
structures
from
readily
accessible
starting
reagents
one
pot.
Thus,
applying
preparation
their
derivatives
can
potent
research
chemistry.
With
rapid
development
various
catalytic
methods
reactions,
this
review
outlines
recent
acid
by
transition
metal‐catalyzed,
light‐mediated
organocatalytic
reactions.
