The VAPB Axis Precisely Coordinates the Timing of Motoneuron Dendritogenesis in Neural Map Development DOI Creative Commons
Daichi Kamiyama,

Yuri Nishida,

Rie Kamiyama

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 31, 2024

Abstract In Drosophila motoneurons, spatiotemporal dendritic patterns are established in the ventral nerve cord. While many guidance cues have been identified, mechanisms of temporal regulation remain unknown. Previously, we identified actin modulator Cdc42 GTPase as a key factor this process. report, further identify upstream factors that activate Cdc42. Using single-cell genetics, FRET-based imaging, and biochemical techniques, demonstrate guanine nucleotide exchange Vav is anchored to plasma membrane via Eph receptor tyrosine kinase, enabling activation. VAMP-associated protein 33 (Vap33), an ligand supplied non-cell-autonomously, may induce autophosphorylation, initiating downstream signaling. Traditionally known ER-resident protein, Vap33 secreted extracellularly at onset activation, acting cue. humans, VAPB—the ortholog Vap33—is similarly spinal cord, its dysregulation leads amyotrophic lateral sclerosis type 8 (ALS8) muscular atrophy (SMA). Our findings provide framework linking VAPB signaling motor circuitry formation both health disease.

Language: Английский

Neuronal wiring diagram of an adult brain DOI Creative Commons
Sven Dorkenwald, Arie Matsliah, Amy Sterling

et al.

Nature, Journal Year: 2024, Volume and Issue: 634(8032), P. 124 - 138

Published: Oct. 2, 2024

Language: Английский

Citations

134
Social context and dopamine signaling converge in the mushroom body to drive impulsivity DOI Open Access
Paul Rafael Sabandal, Young-Cho Kim,

John Martin Sabandal

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 23, 2025

Abstract Organisms adapt their behaviors flexibly in response to various internal and environmental factors. However, how where these factors converge the brain alter behavior is not well understood. In this study, we examine social context interacts with dopamine activity influence inhibitory control Drosophila . We found that, regardless of context—whether isolated or groups—wild-type flies consistently showed strong movement suppression a go/no-go task that measures action restraint. contrast, enhanced suppressed movements when tested alone potential mates but exhibited impulsive exposed same-sex peers. This social-context-dependent impulsivity was shown rely on dopamine-D1 receptor-cAMP signaling mushroom body (MB) neurons. Remarkably, activating MB sufficient induce impulsivity, even without input context. Our findings highlight as critical hub regulate Signficance statement study demonstrates results from interplay between elevated levels context, rather than alone, serving key neural for integrating signals Social stimuli, such presence peers, disrupt context-dependent manner, highlighting importance multimodal sensory inputs activity. These challenge isolation-focused approach traditional research underscore need account influences investigating cognitive processes disorders like ADHD, autism, substance use, settings often amplify symptoms. Classification Genetics / Neuroscience

Language: Английский

Citations

0
An ultrastructural map of a spinal sensorimotor circuit reveals the potential of astroglia modulation DOI Creative Commons

Zachary M. Koh,

Ricky Avalos Arceo,

Jacob Hammer

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

Information flow through circuits is dictated by the precise connectivity of neurons and glia. While a single astrocyte can contact many synapses, how glial-synaptic interactions are arranged within circuit to impact information remains understudied. Here, we use local spinal sensorimotor in zebrafish as model understand astroglia connected vertebrate circuit. With semi-automated cellular reconstructions automated approaches map all synaptic connections, identified connections circuit, from dorsal root ganglia interneurons finally motor neurons. This revealed complex network that interact We then mapped glial processes tripartite synapses demonstrate equally distributed across supporting idea glia modulate at different levels. show multiple astroglia, including bona fide astrocytes, sensory neuron's each these parts detailed reveals an extensive process stimuli vertebrate. utilized this ultrastructural thresholding modulation could alter circuits. validated with GCaMP6s imaging ganglia, astroglia. work provides foundational resource detailing organization offering insights influence neural networks.

Language: Английский

Citations

0
Engrams and causal specificity DOI Creative Commons

Jonathan Najenson

Philosophical Psychology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 27

Published: March 13, 2025

Language: Английский

Citations

0
Relationships and representations of brain structures, connectivity, dynamics and functions DOI
Oliver Schmitt

Progress in Neuro-Psychopharmacology and Biological Psychiatry, Journal Year: 2025, Volume and Issue: unknown, P. 111332 - 111332

Published: March 1, 2025

Language: Английский

Citations

0
A complete wiring diagram of the fruit-fly brain DOI
Anita V. Devineni

Nature, Journal Year: 2024, Volume and Issue: 634(8032), P. 35 - 36

Published: Oct. 2, 2024

Language: Английский

Citations

3
Fly connectome over the wire DOI

Jake Rogers

Nature reviews. Neuroscience, Journal Year: 2024, Volume and Issue: 25(12), P. 757 - 757

Published: Oct. 28, 2024

Language: Английский

Citations

1
Parallel and converging multisensory cascades in the Drosophila connectome DOI Creative Commons
Richard F. Betzel, Maria Grazia Puxeddu, Caio Seguin

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 8, 2024

Connectomes are network maps of synaptic connectivity. A key functional role any connectome is to constrain inter-neuronal signaling and sculpt the flow activity across nervous system. therefore play a central in rapid tranmission information about an organism’s environment from sensory neurons higher-order for action planning ultimately effectors. Here, we use parsimonious model spread investigate connectome’s shaping putative cascades. Our allows us simulate pathways sensors rest brain, mapping similarity these between different modalities identifying convergence zones–neurons that activated simultaneously by modalities. Further, considered two multisensory integration scenarios – cooperative case where interacted “speed up” (reduce) neurons’ activation times competitive “winner take all” case, streams vied same neural territory. Finally, data-driven algorithm partition into classes based on their behavior during cascade simulations. work helps underscore “simple” models enriching data, while offering classification joint connectional/dynamical properties.

Language: Английский

Citations

1
Disentangling how the brain is wired DOI Creative Commons
Simon G. Sprecher

Fly, Journal Year: 2024, Volume and Issue: 19(1)

Published: Dec. 31, 2024

Language: Английский

Citations

0
The VAPB Axis Precisely Coordinates the Timing of Motoneuron Dendritogenesis in Neural Map Development DOI Creative Commons
Daichi Kamiyama,

Yuri Nishida,

Rie Kamiyama

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 31, 2024

Abstract In Drosophila motoneurons, spatiotemporal dendritic patterns are established in the ventral nerve cord. While many guidance cues have been identified, mechanisms of temporal regulation remain unknown. Previously, we identified actin modulator Cdc42 GTPase as a key factor this process. report, further identify upstream factors that activate Cdc42. Using single-cell genetics, FRET-based imaging, and biochemical techniques, demonstrate guanine nucleotide exchange Vav is anchored to plasma membrane via Eph receptor tyrosine kinase, enabling activation. VAMP-associated protein 33 (Vap33), an ligand supplied non-cell-autonomously, may induce autophosphorylation, initiating downstream signaling. Traditionally known ER-resident protein, Vap33 secreted extracellularly at onset activation, acting cue. humans, VAPB—the ortholog Vap33—is similarly spinal cord, its dysregulation leads amyotrophic lateral sclerosis type 8 (ALS8) muscular atrophy (SMA). Our findings provide framework linking VAPB signaling motor circuitry formation both health disease.

Language: Английский

Citations

0