HAND1, partially mediated through ape-specific LTR binding, is essential for human extra-embryonic mesenchyme derivation from iPSCs DOI Creative Commons
Zukai Liu, Yuliana Tan, William F. Flynn

et al.

Cell Reports, Journal Year: 2025, Volume and Issue: 44(4), P. 115568 - 115568

Published: April 1, 2025

The specification of extra-embryonic mesenchyme (ExMC) is a prime example developmental divergence between mouse and human. Derived from definitive mesoderm during gastrulation, the human ExMC first appears at peri-implantation prior to gastrulation therefore its cellular origin, still unknown, must differ. In pluripotent stem cell model, we report that shares progenitor cells with trophoblast, suggesting trophectoderm origin. This ability form extend trophoblast lines. We define HAND1 as an essential regulator specification, null remaining in lineage. Bound by HAND1, ape-specific, endogenous retrovirus-derived LTR2B contributes unique features ExMC. Additionally, supports maintenance cells, possibly reflecting role maintaining epiblast pluripotency through development. Our data emphasize nascent evolutionary innovation early development provide system study this.

Language: Английский

Pooled CRISPR screening with optical sequencing reveals regulators of 3D chromatin organization DOI
Mazhar Adli

Nature Methods, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Language: Английский

Citations

0
Toward automated and explainable high-throughput perturbation analysis in single cells DOI Creative Commons
Jesus Gonzalez-Ferrer, Mohammed A. Mostajo-Radji

Patterns, Journal Year: 2025, Volume and Issue: 6(4), P. 101228 - 101228

Published: April 1, 2025

Language: Английский

Citations

0
HAND1, partially mediated through ape-specific LTR binding, is essential for human extra-embryonic mesenchyme derivation from iPSCs DOI Creative Commons
Zukai Liu, Yuliana Tan, William F. Flynn

et al.

Cell Reports, Journal Year: 2025, Volume and Issue: 44(4), P. 115568 - 115568

Published: April 1, 2025

The specification of extra-embryonic mesenchyme (ExMC) is a prime example developmental divergence between mouse and human. Derived from definitive mesoderm during gastrulation, the human ExMC first appears at peri-implantation prior to gastrulation therefore its cellular origin, still unknown, must differ. In pluripotent stem cell model, we report that shares progenitor cells with trophoblast, suggesting trophectoderm origin. This ability form extend trophoblast lines. We define HAND1 as an essential regulator specification, null remaining in lineage. Bound by HAND1, ape-specific, endogenous retrovirus-derived LTR2B contributes unique features ExMC. Additionally, supports maintenance cells, possibly reflecting role maintaining epiblast pluripotency through development. Our data emphasize nascent evolutionary innovation early development provide system study this.

Language: Английский

Citations

0