Different Species of Bats: Genomics, Transcriptome, and Immune Repertoire DOI Creative Commons

Huifang Wang,

Hao Zhou, Xinsheng Yao

et al.

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(4), P. 252 - 252

Published: April 7, 2025

Bats are the only mammals with ability to fly and second largest order after rodents, 20 families 1213 species (over 3000 subspecies) widely distributed in regions around world except for Antarctica. What makes bats unique their biological traits: a tolerance zoonotic infections without getting clinical symptoms, long lifespans, low incidence of tumors, high metabolism. As result, they receiving increasing attention field life sciences, particularly medical research. The rapid advancements sequencing technology have made it feasible comprehensively analyze diverse characteristics bats. This review discusses following: (1) assembly annotation overview 77 assemblies from 54 across 11 transcriptome 42 7 families, focused on comparative analysis genomic architecture, sensory adaptations (auditory, visual, olfactory), immune functions. Key findings encompass marked interspecies divergence genome size, lineage-specific expansions/contractions immune-related gene (APOBEC, IFN, PYHIN), linked ecological niches. Notably, echolocating exhibited convergent evolution auditory genes (SLC26A5 FOXP2), while fruit-eating displayed degeneration vision-associated (RHO), reflecting trade-offs between specialization demands. (2) V (variable), D (diversity), J (joining), C (constant) TR IG loci 12 five focus differences CDR3 repertoires different other mammals, provides us deeper understanding development function system organisms. Integrated genomic, transcriptomic, repertoire analyses reveal that employ distinct antiviral strategies, primarily mediated by enhanced suppressed inflammatory responses. foundational information, collaboration directions, new perspectives various laboratories conducting basic applied research vast array bat biology.

Language: Английский

Viral tolerance enabled by a bat-specific genomic tweak DOI
Junji Zhu, Michaela U. Gack

Nature, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

Language: Английский

Citations

1
Long-read sequencing and genome assembly of natural history collection samples and challenging specimens DOI Creative Commons

Bernhard Bein,

Ioannis Chrysostomakis, Larissa Souza Arantes

et al.

Genome biology, Journal Year: 2025, Volume and Issue: 26(1)

Published: Feb. 10, 2025

Museum collections harbor millions of samples, largely unutilized for long-read sequencing. Here, we use ethanol-preserved samples containing kilobase-sized DNA to show that amplification-free protocols can yield contiguous genome assemblies. Additionally, using a modified amplification-based protocol, employing an alternative polymerase overcome PCR bias, assemble the 3.1 Gb maned sloth genome, surpassing previous 500 Mb protocol size limit. Our also improves assemblies other difficult-to-sequence molluscs and arthropods, including millimeter-sized organisms. By highlighting as valuable sample resources facilitating assembly tiny challenging organisms, our study advances efforts obtain reference genomes all eukaryotes.

Language: Английский

Citations

1
How flight helped bats become invincible to viruses DOI

Ewen Callaway

Nature, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

Language: Английский

Citations

0
Comparative genomics provides insights into chromosomal evolution and immunological adaptation in horseshoe bats DOI
Shilin Tian,

Jun-Yu Si,

Libiao Zhang

et al.

Nature Ecology & Evolution, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 7, 2025

Language: Английский

Citations

0
Genomic and functional adaptations in guanylate-binding protein 5 (GBP5) highlight specificities of bat antiviral innate immunity DOI Creative Commons

Amandine Le Corf,

Sarah Maesen,

Clara Loyer

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 15, 2025

Bats are asymptomatic reservoirs of several zoonotic viruses. This may result from long-term coevolution between viruses and bats, that have led to host adaptations contributing an effective balance strong antiviral responses with innate immune tolerance. To better understand these virus-host interactions, we combined comparative transcriptomics, phylogenomics functional assays characterize the evolution bat factors. First, stimulated type I interferon pathway in Myotis yumanensis primary cells identified guanylate-binding protein 5 (GBP5) as most differentially expressed interferon-stimulated gene (ISG). Phylogenomic analyses showed GBP5 has been under episodic positive selection, numerous rapidly evolving sites species-specific duplications, suggesting past evolutionary arms races. Functional tests on orthologs ten species covering >60 million years Chiroptera revealed species- virus-specific restrictions against RNA (retrovirus HIV, rhabdoviruses European lyssavirus VSV), which typical signatures viral epidemics. Interestingly, also observed a lineage-specific loss prenylation motif common ancestor Pipistrellus Eptesicus associated different subcellular localization functions. Resurrection ancestral fuscus rescued its localization, but not complete activities, additional determinants necessary for restriction. Altogether, our results highlight contribute specific immunity provide insights into effector GBP5. is upon stimulation cells. Bat evolved genomic genetic diversification, including early stop codon leading truncation motif.GBP5 diversification bats impacts their functions.Bat GBP5s exhibit virus-specificity ability inhibit infectivity particles, bearing glycoproteins retroviral vesicular stomatitis virus lyssavirus-1.Resurrection rescues full activity.

Language: Английский

Citations

0
RNA-Seq analysis reveals the long noncoding RNAs associated with immunity in wild Myotis myotis bats DOI Creative Commons
Sébastien Riquier, Samuel Carthy, Graham M. Hughes

et al.

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: April 5, 2025

Bats possess a uniquely adapted immune system that enables them to live with viral infections without the expected maladies. The molecular basis and regulation of bats' response is still not fully understood. Long non-coding RNAs (lncRNAs) represent an emerging class molecules critical regulatory roles in multiple biological processes, including immunity. We hypothesise lncRNA-based bats may enable limit disease pathogens. developed lncRNA prediction pipeline annotate long transcriptome across bat tissues at population level. Characterisation our dataset based on 100 blood transcriptomes from wild Myotis myotis revealed lower more tissue-specific expression compared coding genes, reduced GC content shorter length distributions, consistent profiles observed other species. Using WGCNA network analyses gene ontology, we identified two mRNA-lncRNA co-expression modules associated distinct response: one linked T-cell activation vial inflammation. From these immune-related lncRNAs, selected four candidates high translational potential for regulating These include newly lncRNA, BatLnc1, antiviral functions; M. ortholog TUG1, implicated viral-host interactions; well-known lncRNAs MALAT1 NEAT1, recognised their inflammatory regulation. conducted first ab initio non-model species, wild-caught myotis. Our analysis significant variation status among subset individuals, potentially due pathogenic conditions. variations, most likely bats. This initial exploration lays groundwork future experimental validations functions, offering promising insights into role

Language: Английский

Citations

0
Different Species of Bats: Genomics, Transcriptome, and Immune Repertoire DOI Creative Commons

Huifang Wang,

Hao Zhou, Xinsheng Yao

et al.

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(4), P. 252 - 252

Published: April 7, 2025

Bats are the only mammals with ability to fly and second largest order after rodents, 20 families 1213 species (over 3000 subspecies) widely distributed in regions around world except for Antarctica. What makes bats unique their biological traits: a tolerance zoonotic infections without getting clinical symptoms, long lifespans, low incidence of tumors, high metabolism. As result, they receiving increasing attention field life sciences, particularly medical research. The rapid advancements sequencing technology have made it feasible comprehensively analyze diverse characteristics bats. This review discusses following: (1) assembly annotation overview 77 assemblies from 54 across 11 transcriptome 42 7 families, focused on comparative analysis genomic architecture, sensory adaptations (auditory, visual, olfactory), immune functions. Key findings encompass marked interspecies divergence genome size, lineage-specific expansions/contractions immune-related gene (APOBEC, IFN, PYHIN), linked ecological niches. Notably, echolocating exhibited convergent evolution auditory genes (SLC26A5 FOXP2), while fruit-eating displayed degeneration vision-associated (RHO), reflecting trade-offs between specialization demands. (2) V (variable), D (diversity), J (joining), C (constant) TR IG loci 12 five focus differences CDR3 repertoires different other mammals, provides us deeper understanding development function system organisms. Integrated genomic, transcriptomic, repertoire analyses reveal that employ distinct antiviral strategies, primarily mediated by enhanced suppressed inflammatory responses. foundational information, collaboration directions, new perspectives various laboratories conducting basic applied research vast array bat biology.

Language: Английский

Citations

0