The fate of artificial transgenes in Acanthamoeba castellanii DOI Creative Commons
Morgan J Colp,

Cédric Blais,

Bruce A. Curtis

et al.

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: April 13, 2025

The soil amoeba Acanthamoeba castellanii is an emerging model organism with which to study a wide range of biomedical, microbiological, and evolutionary phenomena. While transformation systems were established for this more than two decades ago, the fate artificial transgenes has not been well characterized. In study, experiments performed investigate how A. genome responds foreign DNA presented in both circular linear plasmid form. Nanopore sequencing was used as high throughput method screen transgene resulting transformant cultures, candidate integrations identified. Molecular biology validate sequence data provide additional context on transgenes. A devised estimate rate read chimerism nanopore runs accurately account effects identifying putative integrations. Based experimental hand, potential mechanism maintenance proposed, one incoming tandemly duplicated telomeres are added ends. Our results suggest that leads molecules maintained telomere-containing, transgene-bearing minichromosomes, may facilitate chromosomal integration. This process allow lateral gene transfer by expanding window opportunity exogenous be taken up integrated into genome. Similar mechanisms exist other eukaryote groups, suggesting widespread feature biology.

Language: Английский

Diplonemid protists possess exotic endomembrane machinery, impacting models of membrane trafficking in modern and ancient eukaryotes DOI
Kristína Záhonová, Julius Lukeš, Joel B. Dacks

et al.

Current Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

1
The fate of artificial transgenes in Acanthamoeba castellanii DOI Creative Commons
Morgan J Colp,

Cédric Blais,

Bruce A. Curtis

et al.

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: April 13, 2025

The soil amoeba Acanthamoeba castellanii is an emerging model organism with which to study a wide range of biomedical, microbiological, and evolutionary phenomena. While transformation systems were established for this more than two decades ago, the fate artificial transgenes has not been well characterized. In study, experiments performed investigate how A. genome responds foreign DNA presented in both circular linear plasmid form. Nanopore sequencing was used as high throughput method screen transgene resulting transformant cultures, candidate integrations identified. Molecular biology validate sequence data provide additional context on transgenes. A devised estimate rate read chimerism nanopore runs accurately account effects identifying putative integrations. Based experimental hand, potential mechanism maintenance proposed, one incoming tandemly duplicated telomeres are added ends. Our results suggest that leads molecules maintained telomere-containing, transgene-bearing minichromosomes, may facilitate chromosomal integration. This process allow lateral gene transfer by expanding window opportunity exogenous be taken up integrated into genome. Similar mechanisms exist other eukaryote groups, suggesting widespread feature biology.

Language: Английский

Citations

0