Trends in biotechnology,
Journal Year:
2023,
Volume and Issue:
41(3), P. 396 - 409
Published: Jan. 27, 2023
A
series
of
spectacular
scientific
discoveries
and
technological
advances
in
the
second
half
20th
century
have
provided
basis
for
ongoing
genome
editing
revolution.
The
elucidation
structural
functional
features
DNA
RNA
was
followed
by
pioneering
studies
on
editing:
Molecular
biotechnology
born.
Since
then,
four
decades
during
which
progress
insights
methods
continued
at
an
overwhelming
pace.
Fundamental
into
microbial
host-virus
interactions
led
to
development
tools
using
restriction
enzymes
or
revolutionary
CRISPR-Cas
technology.
In
this
review,
we
provide
a
historical
overview
milestones
that
revolution
speculate
about
future
trends
biotechnology.
Science,
Journal Year:
2023,
Volume and Issue:
379(6629)
Published: Jan. 19, 2023
The
advent
of
clustered
regularly
interspaced
short
palindromic
repeat
(CRISPR)
genome
editing,
coupled
with
advances
in
computing
and
imaging
capabilities,
has
initiated
a
new
era
which
genetic
diseases
individual
disease
susceptibilities
are
both
predictable
actionable.
Likewise,
genes
responsible
for
plant
traits
can
be
identified
altered
quickly,
transforming
the
pace
agricultural
research
breeding.
In
this
Review,
we
discuss
current
state
CRISPR-mediated
manipulation
human
cells,
animals,
plants
along
relevant
successes
challenges
present
roadmap
future
technology.
Cell,
Journal Year:
2024,
Volume and Issue:
187(5), P. 1076 - 1100
Published: Feb. 1, 2024
Genome
editing
has
been
a
transformative
force
in
the
life
sciences
and
human
medicine,
offering
unprecedented
opportunities
to
dissect
complex
biological
processes
treat
underlying
causes
of
many
genetic
diseases.
CRISPR-based
technologies,
with
their
remarkable
efficiency
easy
programmability,
stand
at
forefront
this
revolution.
In
Review,
we
discuss
current
state
CRISPR
gene
technologies
both
research
therapy,
highlighting
limitations
that
constrain
them
technological
innovations
have
developed
recent
years
address
them.
Additionally,
examine
summarize
landscape
applications
context
health
therapeutics.
Finally,
outline
potential
future
developments
could
shape
coming
years.
