Advanced Materials,
Journal Year:
2023,
Volume and Issue:
36(8)
Published: Nov. 7, 2023
Bacterial-based
delivery
strategies
have
recently
emerged
as
a
unique
research
direction
in
the
field
of
drug
delivery.
However,
bacterial
vectors
are
quickly
phagocytosed
by
immune
cells
after
entering
bloodstream.
Taking
advantage
this
phenomenon,
herein,
work
seeks
to
harness
potential
micron-sized
vectors,
and
find
that
inactivated
can
accumulate
at
tumor-site
intravenous
injection
through
CD11b
Science,
Journal Year:
2022,
Volume and Issue:
378(6622), P. 858 - 864
Published: Nov. 24, 2022
With
increasing
evidence
that
microbes
colonize
tumors,
synthetic
biology
tools
are
being
leveraged
to
repurpose
bacteria
as
tumor-specific
delivery
systems.
These
engineered
systems
can
modulate
the
tumor
microenvironment
using
a
combination
of
their
inherent
immunogenicity
and
local
payload
production.
Here,
we
review
genetic
circuits
enhance
spatial
temporal
control
therapeutic
improve
safety
efficacy.
We
describe
engineering
interactions
among
bacteria,
cells,
immune
progression
from
single
agents
toward
rational
with
other
modalities.
Together,
these
efforts
building
an
emerging
concept
between
programmable
medicines
biology.
Science Advances,
Journal Year:
2022,
Volume and Issue:
8(28)
Published: July 15, 2022
Bacterial
biohybrids,
composed
of
self-propelling
bacteria
carrying
micro/nanoscale
materials,
can
deliver
their
payload
to
specific
regions
under
magnetic
control,
enabling
additional
frontiers
in
minimally
invasive
medicine.
However,
current
bacterial
biohybrid
designs
lack
high-throughput
and
facile
construction
with
favorable
cargoes,
thus
underperforming
terms
propulsion,
efficiency,
tissue
penetration,
spatiotemporal
operation.
Here,
we
report
magnetically
controlled
biohybrids
for
targeted
localization
multistimuli-responsive
drug
release
three-dimensional
(3D)
biological
matrices.
Magnetic
nanoparticles
nanoliposomes
loaded
photothermal
agents
chemotherapeutic
molecules
were
integrated
onto
Escherichia
coli
~90%
efficiency.
outperforming
previously
reported
E.
–based
microrobots,
retained
original
motility
able
navigate
through
matrices
colonize
tumor
spheroids
fields
on-demand
the
by
near-infrared
stimulus.
Our
work
provides
a
multifunctional
microrobotic
platform
guided
locomotion
3D
networks
stimuli-responsive
delivery
therapeutics
diverse
medical
applications.
Chemical Reviews,
Journal Year:
2022,
Volume and Issue:
123(5), P. 2349 - 2419
Published: Dec. 13, 2022
Recent
advances
in
synthetic
biology
and
materials
science
have
given
rise
to
a
new
form
of
materials,
namely
engineered
living
(ELMs),
which
are
composed
matter
or
cell
communities
embedded
self-regenerating
matrices
their
own
artificial
scaffolds.
Like
natural
such
as
bone,
wood,
skin,
ELMs,
possess
the
functional
capabilities
organisms,
can
grow,
self-organize,
self-repair
when
needed.
They
also
spontaneously
perform
programmed
biological
functions
upon
sensing
external
cues.
Currently,
ELMs
show
promise
for
green
energy
production,
bioremediation,
disease
treatment,
fabricating
advanced
smart
materials.
This
review
first
introduces
dynamic
features
systems
potential
developing
novel
We
then
summarize
recent
research
progress
on
emerging
design
strategies
from
both
perspectives.
Finally,
we
discuss
positive
impacts
promoting
sustainability
key
future
directions.
Science,
Journal Year:
2023,
Volume and Issue:
382(6667), P. 211 - 218
Published: Oct. 12, 2023
A
major
challenge
facing
tumor-antigen
targeting
therapies
such
as
chimeric
antigen
receptor
(CAR)-T
cells
is
the
identification
of
suitable
targets
that
are
specifically
and
uniformly
expressed
on
heterogeneous
solid
tumors.
By
contrast,
certain
species
bacteria
selectively
colonize
immune-privileged
tumor
cores
can
be
engineered
antigen-independent
platforms
for
therapeutic
delivery.
To
bridge
these
approaches,
we
developed
a
platform
probiotic-guided
CAR-T
(ProCARs),
in
which
tumor-colonizing
probiotics
release
synthetic
label
tissue
CAR-mediated
lysis
situ.
This
system
demonstrated
cell
activation
antigen-agnostic
was
safe
effective
multiple
xenograft
syngeneic
models
human
mouse
cancers.
We
further
multifunctional
co-release
chemokines
to
enhance
recruitment
response.
Angewandte Chemie International Edition,
Journal Year:
2022,
Volume and Issue:
61(27)
Published: April 11, 2022
An
approach
of
decorating
bacteria
with
triple
immune
nanoactivators
is
reported
to
develop
tumor-resident
living
immunotherapeutics.
Under
cytocompatible
conditions,
tumor-specific
antigens
and
checkpoint
blocking
antibodies
are
simultaneously
conjugated
onto
bacterial
surface
then
polydopamine
nanoparticles
formed
via
in
situ
dopamine
polymerization.
In
addition
serving
as
a
linker,
its
photothermal
effect
can
repolarize
tumor-associated
macrophages
pro-inflammatory
phenotype.
The
linked
promote
the
maturation
dendritic
cells
generate
responses,
while
anchored
block
checkpoints
activate
cytotoxic
T
lymphocytes.
Decorated
show
spatiotemporal
tumor
retention
proliferation-dependent
drug
release,
achieving
potent
antitumor
effects
two
antigen-overexpressing
models.
This
work
provides
versatile
platform
prepare
multimodal
long-acting
therapeutics
for
cancer
immunotherapy.
Nature Communications,
Journal Year:
2022,
Volume and Issue:
13(1)
Published: Oct. 24, 2022
Pore-forming
Gasdermin
protein-induced
pyroptosis
in
tumor
cells
promotes
anti-tumor
immune
response
through
the
release
of
pro-inflammatory
cytokines
and
immunogenic
substances
after
cell
rupture.
However,
endosomal
sorting
complexes
required
for
transport
(ESCRT)
III-mediated
membrane
repair
significantly
diminishes
by
repairing
subsequently
removing
gasdermin
pores.
Here,
we
show
that
blocking
calcium
influx-triggered
ESCRT
III-dependent
a
biodegradable
nanoparticle-mediated
sustained
chelator
(EI-NP)
strongly
enhances
intracellularly
delivered
GSDMD-induced
via
bacteria-based
delivery
system
(VNP-GD).
An
injectable
hydrogel
lyophilized
hydrogel-based
patch
are
developed
peritumoral
administration
treating
primary
metastatic
tumors,
implantation
inoperable
tumors
respectively.
The
hydrogels,
functioning
as
local
therapeutic
reservoirs,
can
sustainedly
VNP-GD
to
effectively
trigger
EI-NP
prevent
III-induced
plasma
boost
effects,
working
synergistically
augment
response.
ACS Nano,
Journal Year:
2023,
Volume and Issue:
17(4), P. 3225 - 3258
Published: Feb. 6, 2023
The
immune
checkpoint
blockade
(ICB)
therapy
has
revolutionized
the
field
of
cancer
treatment,
while
low
response
rates
and
systemic
toxicity
limit
its
clinical
outcomes.
With
rapid
advances
in
nanotechnology
materials
science,
various
types
biomaterials
have
been
developed
to
maximize
therapeutic
efficacy
minimizing
side
effects
by
increasing
tumor
antigenicity,
reversing
immunosuppressive
microenvironment,
amplifying
antitumor
response,
reducing
extratumoral
distribution
inhibitors
as
well
enhancing
their
retention
within
target
sites.
In
this
review,
we
reviewed
current
design
strategies
for
different
augment
ICB
effectively
then
discussed
present
representative
biomaterial-assisted
modulation
targeted
delivery
boost
therapy.
Current
challenges
future
development
prospects
expanding
with
were
also
summarized.
We
anticipate
review
will
be
helpful
developing
emerging
promoting
application
Nature Reviews Bioengineering,
Journal Year:
2023,
Volume and Issue:
1(3), P. 208 - 225
Published: Feb. 2, 2023
Oral
administration
is
a
widespread
and
convenient
drug
delivery
approach.
However,
oral
can
be
affected
by
the
complex
digestive
tract
environment,
including
irregular
tissue
morphology,
presence
of
enzymes,
mucus
mucosal
barriers,
spatiotemporal
variance
in
physiological
parameters.
These
obstacles
prevent
many
therapeutics.
To
overcome
these
challenges,
devices
designed
with
bioinspired
compositions,
structures
or
functions
to
make
more
drugs
available
for
administration.
Various
have
been
developed
harnessing
biological
materials
living
microorganisms,
imitating
functions.
In
this
Review,
we
discuss
design
modification
devices,
examining
engineering
strategies
target
specific
tissues
applications.
We
highlight
how
key
bottlenecks
addressed
through
designs,
concluding
an
outlook
on
remaining
challenges
towards
clinical
translation
devices.
commonly
used
strategy
deliver
harsh
environment
prevents
negatively
affect
efficacy.
This
Review
discusses
microorganisms
into
that
associated
delivery.
