The
increasing
use
of
tissue
clearing
techniques
underscores
the
urgent
need
for
cost-effective
and
simplified
deep
imaging
methods.
While
traditional
inverted
confocal
microscopes
excel
in
high-resolution
sections
cultured
cells,
they
face
limitations
cleared
tissues
due
to
refractive
index
mismatches
between
immersion
media
objectives
sample
container.
To
overcome
these
challenges,
RIM-Deep
was
developed
significantly
improve
capabilities
without
compromising
normal
function
microscope.
This
system
facilitates
immunofluorescence
prefrontal
cortex
macaque
tissue,
extending
depth
from
2
mm
5
mm.
Applied
an
intact
Thy1-EGFP
mouse
brain,
allowed
clear
axonal
visualization
at
high
depth.
Moreover,
this
advancement
enables
large-scale,
3D
tissues.
In
principle,
concept
can
be
extended
any
modality,
including
existing
wide-field,
confocal,
two-photon
microscopy.
would
upgrade
laboratory
configurations
facilitate
study
connectomics
brain
other
Nature Methods,
Journal Year:
2023,
Volume and Issue:
21(1), P. 132 - 141
Published: Dec. 21, 2023
Abstract
Multiphoton
microscopy
can
resolve
fluorescent
structures
and
dynamics
deep
in
scattering
tissue
has
transformed
neural
imaging,
but
applying
this
technique
vivo
be
limited
by
the
mechanical
optical
constraints
of
conventional
objectives.
Short
working
distance
objectives
collide
with
compact
surgical
windows
or
other
instrumentation
preclude
imaging.
Here
we
present
an
ultra-long
(20
mm)
air
objective
called
Cousa
objective.
It
is
optimized
for
performance
across
multiphoton
imaging
wavelengths,
offers
a
more
than
4
mm
2
field
view
submicrometer
lateral
resolution
compatible
commonly
used
systems.
A
novel
design,
wider
typical
microscope
objectives,
enabled
combination
specifications.
We
share
full
prescription,
report
including
two-photon
three-photon
array
species
preparations,
nonhuman
primates.
The
enable
range
experiments
neuroscience
beyond.
Advanced Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 14, 2024
In
robotics,
particularly
for
autonomous
navigation
and
human-robot
collaboration,
the
significance
of
unconventional
imaging
techniques
efficient
data
processing
capabilities
is
paramount.
The
unstructured
environments
encountered
by
robots,
coupled
with
complex
missions
assigned
to
them,
present
numerous
challenges
necessitating
diverse
visual
functionalities,
consequently,
development
multifunctional
robotic
vision
systems
has
become
indispensable.
Meanwhile,
rich
diversity
inherent
in
animal
systems,
honed
over
evolutionary
epochs
meet
their
survival
demands
across
varied
habitats,
serves
as
a
profound
source
inspirations.
Here,
recent
advancements
drawing
inspiration
from
natural
ocular
structures
perception
mechanisms
are
delineated.
First,
unique
functionalities
eyes
terrestrial,
aerial,
aquatic
habitats
signal
mechanism
humans
explored.
Then,
designs
bio-inspired
electronic
explored,
engineered
mimic
key
components
underlying
optical
principles
eyes.
Furthermore,
neuromorphic
image
sensors
discussed,
emulating
functional
properties
synapses,
neurons,
retinas
thereby
enhancing
accuracy
efficiency
tasks.
Next,
integration
examples
mobile
robotic/biological
introduced.
Finally,
forward-looking
outlook
on
provided.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: June 9, 2023
Abstract
Recent
advances
in
tissue
processing,
labeling,
and
fluorescence
microscopy
are
providing
unprecedented
views
of
the
structure
cells
tissues
at
sub-diffraction
resolutions
near
single
molecule
sensitivity,
driving
discoveries
diverse
fields
biology,
including
neuroscience.
Biological
is
organized
over
scales
nanometers
to
centimeters.
Harnessing
molecular
imaging
across
intact,
three-dimensional
samples
on
this
scale
requires
new
types
microscopes
with
larger
view
working
distance,
as
well
higher
throughput.
We
present
a
expansion-assisted
selective
plane
illumination
microscope
(ExA-SPIM)
aberration-free
1×1×3
μm
optical
resolution
large
field
(10.6×8.0
mm
2
)
distance
(35
mm)
speeds
up
946
megavoxels/sec.
Combined
clearing
expansion
methods,
allows
centimeter-scale
250×250×750
nm
(4×
expansion),
entire
mouse
brains,
high
contrast
without
sectioning.
illustrate
ExA-SPIM
by
reconstructing
individual
neurons
brain,
cortico-spinal
macaque
motor
cortex,
visualizing
axons
human
white
matter.
International Journal of Optomechatronics,
Journal Year:
2024,
Volume and Issue:
18(1)
Published: April 14, 2024
In
the
realm
of
optical
technologies,
integration
nature's
designs
and
modern
engineering
has
paved
way
for
groundbreaking
innovations.
Bio-inspired
tunable
optics
photonics,
drawing
from
intricate
mechanisms
found
in
biological
systems,
offer
a
new
frontier
adaptive
efficient
light
management.
Here,
this
review
presents
comprehensive
examination
principles,
advancements,
applications
natural
light-manipulation
adaptation
mechanisms,
highlighting
their
translation
into
artificial
photonic
structures.
Emphasizing
remarkable
potential
bio-inspired
particularly
those
emulating
functionalities
eyes
skins,
it
explores
current
state
devices.
Our
categorizes
these
systems
two
foundational
mechanisms:
light-adaptation,
illustrating
wide-ranging
implications
consumer
electronics
to
next-generation
technologies.
This
also
highlights
challenges
prospects
photonics.
It
emphasizes
role
promoting
properties
multifunctional
devices,
providing
revolutionary
opportunities
across
various
sectors,
including
military
everyday
life,
thus
surpassing
cutting-edge
BME Frontiers,
Journal Year:
2025,
Volume and Issue:
6
Published: Jan. 1, 2025
Combining
transparent
embedding
with
sectioning
is
likely
to
be
the
future
direction
for
tissue
clearing
and
3-dimensional
(3D)
imaging.
A
newly
published
system,
TESOS
(Transparent
Embedding
Solvent
System),
ensures
consistent
submicron
resolution
imaging
throughout
entire
sample,
can
compatible
different
microscopy
systems.
This
method
shows
great
potential
in
connectome
mapping,
might
an
optimal
option
3D
multiplex
immunofluorescence
RNA
situ
hybridization
Additional
efforts
would
needed
innovate
labeling,
imaging,
data
processing
strategies
fully
utilize
of
systems
high-resolution
large-scale
samples.
The
increasing
use
of
tissue
clearing
techniques
underscores
the
urgent
need
for
cost-effective
and
simplified
deep
imaging
methods.
While
traditional
inverted
confocal
microscopes
excel
in
high-resolution
sections
cultured
cells,
they
face
limitations
cleared
tissues
due
to
refractive
index
mismatches
between
immersion
media
objectives
sample
container.
To
overcome
these
challenges,
RIM-Deep
was
developed
significantly
improve
capabilities
without
compromising
normal
function
microscope.
This
system
facilitates
immunofluorescence
prefrontal
cortex
macaque
tissue,
extending
depth
from
2
mm
5
mm.
Applied
an
intact
Thy1-EGFP
mouse
brain,
allowed
clear
axonal
visualization
at
high
depth.
Moreover,
this
advancement
enables
large-scale,
3D
tissues.
In
principle,
concept
can
be
extended
any
modality,
including
existing
wide-field,
confocal,
two-photon
microscopy.
would
upgrade
laboratory
configurations
facilitate
study
connectomics
brain
other
The
increasing
use
of
tissue
clearing
techniques
underscores
the
urgent
need
for
cost-effective
and
simplified
deep
imaging
methods.
While
traditional
inverted
confocal
microscopes
excel
in
high-resolution
sections
cultured
cells,
they
face
limitations
cleared
tissues
due
to
refractive
index
mismatches
between
immersion
media
objectives
sample
container.
To
overcome
these
challenges,
RIM-Deep
was
developed
significantly
improve
capabilities
without
compromising
normal
function
microscope.
This
system
facilitates
immunofluorescence
prefrontal
cortex
macaque
tissue,
extending
depth
from
2
mm
5
mm.
Applied
an
intact
Thy1-EGFP
mouse
brain,
allowed
clear
axonal
visualization
at
high
depth.
Moreover,
this
advancement
enables
large-scale,
3D
tissues.
In
principle,
concept
can
be
extended
any
modality,
including
existing
wide-field,
confocal,
two-photon
microscopy.
would
upgrade
laboratory
configurations
facilitate
study
connectomes
brain
other
