Growth-coupled microbial biosynthesis of the animal pigment xanthommatin DOI Open Access
Leah B. Bushin, Tobias B. Alter,

María V.G. Alván-Vargas

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 6, 2024

Abstract The mining of genomes across life has unearthed a bounty biosynthetic potential to diverse molecules key biobased future. While the heterologous expression metabolic pathways achieved broad success, most approaches suffer similar fate in low initial production levels that require extensive, resource-heavy iterative strain engineering refinement. Herein we introduce growth-coupled (GrowBio) strategy irrevocably connects microbial growth with specialized compound production. We demonstrate plug-and-play versatility GrowBio structurally complex animal biopigment xanthommatin, color-changing ommochrome material and cosmetic potential. Xanthommatin biosynthesis directly fuels newly designed Pseudomonas putida 5,10-methylenetetrahydrofolate auxotroph (PUMA). Aided by genome-scale modeling, PUMA was built be controlled endogenous formate co-produced as coupled byproduct multistep conversion tryptophan xanthommatin. Adaptive laboratory evolution utilized streamline xanthommatin’s gram-scale bioproduction via rate selection, establishing promising biotechnological approach for optimizing value-added molecules.

Language: Английский

Delivering living medicines with biomaterials DOI
Tetsuhiro Harimoto, Wei‐Hung Jung, David Mooney

et al.

Nature Reviews Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

Language: Английский

Citations

3
From Nature to Lab: Sustainable Bacterial Cellulose Production and Modification with Synthetic Biology DOI Open Access

Vid Potočnik,

Selestina Gorgieva, Janja Trček

et al.

Polymers, Journal Year: 2023, Volume and Issue: 15(16), P. 3466 - 3466

Published: Aug. 18, 2023

Bacterial cellulose (BC) is a macromolecule with versatile applications in medicine, pharmacy, biotechnology, cosmetology, food and packaging, ecology, electronics. Although many bacteria synthesize BC, the most efficient BC producers are certain species of genera

Language: Английский

Citations

32
Overview of indigo biosynthesis by Flavin-containing Monooxygenases: History, industrialization challenges, and strategies DOI
Changxin Fan,

Ziqi Xie,

Da Zheng

et al.

Biotechnology Advances, Journal Year: 2024, Volume and Issue: 73, P. 108374 - 108374

Published: May 8, 2024

Language: Английский

Citations

7
Programmable Bacterial Biofilms as Engineered Living Materials DOI Creative Commons
Yan‐Yi Wang, Qian Zhang, Changhao Ge

et al.

Accounts of Materials Research, Journal Year: 2024, Volume and Issue: 5(7), P. 797 - 808

Published: June 15, 2024

ConspectusBiological substances like wood and bone demonstrate extraordinary characteristics of "living" features, such as the ability to self-grow, self-heal upon encountering damage, sense adapt environmental changes. These attributes are crucial for their survival adaptation in complex environments. In field material science, there is a growing interest developing biomimetic materials that can self-monitor, conditions, self-repair when necessary. Such capabilities would extend lifespan pave way intelligent applications. However, creating with autonomy intelligence on par biological systems remains daunting challenge. this context, synthetic biology offers promising avenue. It not only allows harnessing inherent dynamic properties living organisms but provides possibility imparting additional advanced functionalities beyond reach systems. This approach enables integration cells into materials, providing them naturally endowed or artificially designed traits. innovative known Engineered Living Materials (ELMs), represent an emerging category smart capable autonomous functions, applications varying from biomedicine sustainable technology.Microbial biofilms, owing self-organizing serve exemplary starting point ELMs. Biofilms consist communities microorganisms residing within three-dimensional (3D) extracellular matrices polymeric (EPS). offer ideal blueprint designing ELMs, attributing remarkable stability, enhanced resilience against severe genetic programmability EPS components. Various biofilm-based have been developed using biofilm components structural proteins, bacterial cellulose, fungal mycelium, ranging pollution remediation, building construction, clean energy generation, biomedicine. Drawing traits shared natural systems, those ELMs divided three main groups: environmentally responsive composite materials. Self-organizing created by genetically altering components, giving rise new functions while maintaining intrinsic hierarchical self-assembling features biofilms. Environmentally harboring gene circuits enable monitor external conditions respond particular cues. High-performance integrate modified biofilms nonliving artificial substances, unique benefits both account overview these categories highlighting respective design strategies significant By combining principles science biology, potential create adaptive properties. Account also addresses challenges prospects associated intending spark ideas foster interdisciplinary collaborations field.

Language: Английский

Citations

6
Bacterial cellulose-graphene oxide composite membranes with enhanced fouling resistance for bio-effluents management DOI Creative Commons
Ishfaq Showket Mir, Ali Riaz, Julie Fréchette

et al.

npj Clean Water, Journal Year: 2024, Volume and Issue: 7(1)

Published: Oct. 25, 2024

Bacterial cellulose composites hold promise as renewable bioinspired materials for industrial and environmental applications. However, their use free-standing water filtration membranes is hindered by low compressive strength, fouling, poor contaminant selectivity. This study investigates the potential of bacterial cellulose-graphene oxide fouling resistance in pressure-driven filtration. Graphene dispersed poly(ethylene glycol) (PEG-400) incorporated a reinforcing filler into 3D network using an in-situ synthesis method. The effect graphene on situ fermentation yield formation percolated-network shows that optimal membrane properties are reached at loading 2 mg/mL. two-dimensional nanosheets uniformly matrix nanofibers via hydrogen-bonded interactions demonstrated nearly twofold higher flux (380 L m−2 h−1) with molecular weight cut-off ranging between 100–200 KDa sixfold increase wet compression strength than pristine BC. When exposed to synthetic organic foulants rich feed solutions, composite showed more 95% recovery. Additionally, achieved over rejection natural matter showcasing enhanced

Language: Английский

Citations

4
Living Dual Heat- and pH-Responsive Textiles DOI

Dalia Jane Saldanha,

Simon James Alexander Rowat,

Henry Stephenson

et al.

ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

Smart textiles that integrate multiple environmental sensing capabilities are an emerging frontier in wearable technology. In this study, we developed dual pH- and temperature-responsive by combining engineered bacterial systems with bacterially derived proteins. For temperature sensing, characterized the properties of a heat sensitive promoter, Phs, Escherichia coli (E. coli) using enhanced green fluorescent protein as reporter. Our findings demonstrate Phs promoter drives elevated gene expression at temperatures between 37 43 °C, maintaining sustained activity for several hours. Moreover, found short shocks can significantly boost levels promoter. We successfully integrated E. expressing Phs-EGFP cells onto confirmed their ability to retain heat-responsive behavior after integration. To achieve pH responsiveness, utilized curli fibers, genetically incorporate pH-sensitive protein, pHuji. pH-sensing fibers proteins have proven track record creating stable bioresponsive textile coatings. By embedding Phs-EGFP-expressing bacteria within fiber coatings, created dual-responsive capable differentiating acidic alkaline environments while simultaneously responding thermal stimuli. These multifunctional exhibited response capabilities. This work establishes proof-of-concept smart living modular functionalities, paving way toward advanced materials.

Language: Английский

Citations

0
Flavoaffinins, elusive cellulose-binding natural products from an anaerobic bacterium DOI Creative Commons
Duncan J. Kountz, Ruocheng Yu, Jessie H. Lee

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 8, 2025

ABSTRACT Cellulose is the most abundant polymer on earth and plays a key role in carbon cycle, agriculture, human health. Many anaerobic cellulose-degrading bacteria produce uncharacterized yellow-orange, cellulose-binding pigments known as yellow affinity substances (here referred to flavoaffinins) that are associated with efficient cellulose degradation. Here, we isolate structurally characterize flavoaffinins from Clostridium ( Hungateiclostridium ) thermocellum , workhorse for industrial conversion of cellulosic feedstocks ethanol. Flavoaffinins represent an unprecedented structural juxtaposition aryl polyene chain hydroxy-diene γ-lactone. We also shed light their biosynthetic origins using stable-isotope feeding experiments. This effort lays groundwork understanding biological function(s) expands limited number natural products isolated obligately microbes. Abstract Figure

Language: Английский

Citations

0
Engineered Living Energy Materials DOI Creative Commons
Xinyi Yuan, Haiyi Xu, Xingwu Liu

et al.

Interdisciplinary materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

ABSTRACT To foster sustainable development, a pivotal trend lies in harnessing energy supplies that propel modern economic and societal progress. Recent advancements living materials for applications have sparked groundbreaking research area: engineered (ELEMs), which seamlessly integrate biological artificial systems efficient conversion storage. consolidate this area, herein, we summarize delve into the evolution of ELEMs. Firstly, provide an overview structural features mechanisms employed by bio‐modules spanning proteins, organelles, entire organisms. They can be directly used as components constructing ELEMs or inspirations design such entities. Then, comprehensively review latest strides based on their distinct modes. Finally, discuss challenges confronting envision future trajectories. The progress holds immense potential to catalyze interdisciplinary endeavors encompassing medicine, environmental science, technologies.

Language: Английский

Citations

0
Environmentally Conscious Hydrophobic Spray Coatings on Bacterial Cellulose for Sustainable and Reusable Textiles DOI
Katie Gilmour, Bruna Martins Dellagnezze, G.E. Bonney

et al.

Journal of Cleaner Production, Journal Year: 2025, Volume and Issue: unknown, P. 145775 - 145775

Published: May 1, 2025

Language: Английский

Citations

0
CRISPR-guided base editor enables efficient and multiplex genome editing in bacterial cellulose-producing Komagataeibacter species DOI
Bo Xin, Jiaheng Liu,

Jinyang Li

et al.

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

ABSTRACT Bacterial cellulose (BC) is an extracellular polysaccharide produced by bacteria that has wide applications in the food industry, tissue engineering, and battery manufacturing. Genome editing of BC-producing Komagataeibacter species expected to optimize BC production its properties. However, available technology can target only one gene at a time requires foreign DNA templates, which may present regulatory hurdle for genetically modified organisms. In this study, we developed clustered regularly interspaced short palindromic repeats (CRISPR)-guided base method using Cas9 nickase cytidine deaminase. Without C-to-T conversions were performed within 8 bp window with 90% efficiency. Double- triple-gene was achieved 80%–90% Fusing uracil-DNA glycosylase editor enabled C-to-G editing. The worked efficiently various species. Finally, mannitol metabolic genes investigated base-editing-mediated inactivation. This study provides powerful tool multiplex genome IMPORTANCE , bacterial genus belonging family Acetobacteraceae important material biosynthesis. relies on traditional homologous recombination methods. Therefore, be manipulated each round organisms when microorganisms are used industry. implemented up three loci genome. system accelerate basic applied research

Language: Английский

Citations

0