Genome-assisted identification of wheat leaf rust resistance gene Lr30 (synonym Lr.ace-4A) DOI Creative Commons
Shisheng Chen,

Jinwei Yang,

Hongna Li

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Abstract Leaf rust is a devastating fungal disease of wheat. Planting resistant wheat cultivars the most effective strategy to mitigate this threat. Here, we generate 10.51-gigabase chromosome-scale assembly durum landrace PI 192051. Using mutagenesis and transcriptome sequencing, identify leaf resistance gene Lr.ace-4A within recombination-sparse region 192051 demonstrate that identical previously designated Lr30 in hexaploid Lr30/Lr.ace-4A encodes non-canonical coiled-coil nucleotide-binding leucine-rich repeat receptor, featuring tandem NB-ARC domains. This proves both necessary sufficient confer Puccinia triticina, as demonstrated by CRISPR/Cas9-induced mutations transgenic complementation. provides near-immunity wheat, though its effectiveness diminished Two amino acid polymorphisms differentiate susceptible haplotypes, with plants carrying either variant exhibiting susceptibility. Cloning will accelerate deployment breeding programs.

Language: Английский

The wheat stem rust resistance gene Sr43 encodes an unusual protein kinase DOI Creative Commons
Guotai Yu, Oadi Matny, Spyridon Gourdoupis

et al.

Nature Genetics, Journal Year: 2023, Volume and Issue: 55(6), P. 921 - 926

Published: May 22, 2023

Abstract To safeguard bread wheat against pests and diseases, breeders have introduced over 200 resistance genes into its genome, thus nearly doubling the number of designated in gene pool 1 . Isolating these facilitates their fast-tracking breeding programs incorporation polygene stacks for more durable resistance. We cloned stem rust Sr43 , which was crossed from wild grass Thinopyrum elongatum 2,3 encodes an active protein kinase fused to two domains unknown function. The gene, is unique Triticeae, appears arisen through a fusion event 6.7 11.6 million years ago. Transgenic expression conferred high levels wide range isolates pathogen causing rust, highlighting potential value engineering.

Language: Английский

Citations

56
Dissection of a rapidly evolving wheat resistance gene cluster by long-read genome sequencing accelerated the cloning of Pm69 DOI Creative Commons
Yinghui Li, Zhen-Zhen Wei, Hanan Sela

et al.

Plant Communications, Journal Year: 2023, Volume and Issue: 5(1), P. 100646 - 100646

Published: July 6, 2023

Gene cloning in repeat-rich polyploid genomes remains challenging. Here we describe a strategy for overcoming major bottlenecks the of powdery mildew (Pm) resistance gene (R-gene) Pm69 derived from tetraploid wild emmer wheat (WEW). A conventional positional approach was not effective due to suppressed recombination. Chromosome sorting compromised by insufficient purity. physical map, constructed assembling Oxford Nanopore Technology (ONT) long-read genome sequences, revealed rapidly evolving nucleotide-binding leucine-rich repeat (NLR) R-gene cluster with structural variations. single candidate NLR identified anchoring RNASeq reads susceptible mutants ONT contigs and validated virus-induced silencing. is likely newly evolved NLR, which discovered only one location across WEW distribution range Israel. successfully introgressed into cultivated wheat, diagnostic molecular marker used accelerate its deployment pyramiding other R-genes.

Language: Английский

Citations

56
Plant pangenomes for crop improvement, biodiversity and evolution DOI
Mona Schreiber, Murukarthick Jayakodi, Nils Stein

et al.

Nature Reviews Genetics, Journal Year: 2024, Volume and Issue: 25(8), P. 563 - 577

Published: Feb. 20, 2024

Language: Английский

Citations

30
A membrane associated tandem kinase from wild emmer wheat confers broad-spectrum resistance to powdery mildew DOI Creative Commons
Miaomiao Li, Huaizhi Zhang,

Huixin Xiao

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: April 10, 2024

Abstract Crop wild relatives offer natural variations of disease resistance for crop improvement. Here, we report the isolation broad-spectrum powdery mildew gene Pm36 , originated from emmer wheat, that encodes a tandem kinase with transmembrane domain (WTK7-TM) through combination map-based cloning, PacBio SMRT long-read genome sequencing, mutagenesis, and transformation. Mutagenesis assay reveals two domains WTK7-TM are critical function. Consistently, in vitro phosphorylation shows indispensable activity WTK7-TM. Haplotype analysis uncovers is an orphan only present few indicating its single ancient origin potential contribution to current wheat pool. Overall, our findings not provide great breeding but also sheds light into mechanism underlying resistance.

Language: Английский

Citations

22
Wheat powdery mildew resistance gene Pm13 encodes a mixed lineage kinase domain-like protein DOI Creative Commons
Huanhuan Li,

Wenqiang Men,

Chao Ma

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 19, 2024

Abstract Wheat powdery mildew is one of the most destructive diseases threatening global wheat production. The wild relatives constitute rich sources diversity for resistance. Here, we report map-based cloning resistance gene Pm13 from species Aegilops longissima . encodes a mixed lineage kinase domain-like (MLKL) protein that contains an N-terminal-domain MLKL (MLKL_NTD) domain in its N-terminus and C-terminal serine/threonine (STK) domain. function validated by mutagenesis, silencing, transgenic assay, allelic association analyses. development introgression lines with significantly reduced chromosome segments Ae. encompassing enables widespread deployment this into cultivars. may provide valuable insights molecular mechanisms underlying -mediated highlight important roles fusion proteins (KFPs) immunity.

Language: Английский

Citations

21
Pm57 from Aegilops searsii encodes a tandem kinase protein and confers wheat powdery mildew resistance DOI Creative Commons
Yue Zhao,

Zhenjie Dong,

Jingnan Miao

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: June 5, 2024

Abstract Powdery mildew is a devastating disease that affects wheat yield and quality. Wheat wild relatives represent valuable sources of resistance genes. Cloning characterization these genes will facilitate their incorporation into breeding programs. Here, we report the cloning Pm57 , powdery gene from Aegilops searsii . It encodes tandem kinase protein with putative kinase-pseudokinase domains followed by von Willebrand factor A domain (WTK-vWA), being ortholog Lr9 mediates leaf rust resistance. The function validated via independent mutants, silencing, transgenic assays. Stable lines introgression exhibit high levels all-stage to diverse isolates Bgt fungus, no negative impacts on agronomic parameters are observed in our experimental set-up. Our findings highlight emerging role fusion proteins plant provide for breeding.

Language: Английский

Citations

17
Origin and evolution of the bread wheat D genome DOI Creative Commons
Emile Cavalet-Giorsa, Andrea González-Muñoz, Naveenkumar Athiyannan

et al.

Nature, Journal Year: 2024, Volume and Issue: 633(8031), P. 848 - 855

Published: Aug. 14, 2024

Bread wheat (Triticum aestivum) is a globally dominant crop and major source of calories proteins for the human diet. Compared with its wild ancestors, modern bread shows lower genetic diversity, caused by polyploidisation, domestication breeding bottlenecks

Language: Английский

Citations

16
Tandem kinase proteins across the plant kingdom DOI
Tamara Reveguk, Andrii Fatiukha,

Evgenii Potapenko

et al.

Nature Genetics, Journal Year: 2025, Volume and Issue: 57(1), P. 254 - 262

Published: Jan. 1, 2025

Language: Английский

Citations

3
Sequencing trait-associated mutations to clone wheat rust-resistance gene YrNAM DOI Creative Commons
Fei Ni, Yanyan Zheng, Xiaoke Liu

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: July 19, 2023

Abstract Stripe (yellow) rust, caused by Puccinia striiformis f. sp. tritici ( Pst ), can significantly affect wheat production. Cloning resistance genes is critical for efficient and effective breeding of stripe rust resistant cultivars. One gene Yr10 CG ) underlying the locus has been cloned. However, following haplotype linkage analyses indicate presence additional gene(s) underlying/near locus. Here, we report cloning YrNAM in this region using method sequencing trait-associated mutations (STAM). encodes a non-canonical protein with NAM domain ZnF-BED domain. We show that both domains are required resistance. Transgenic harboring driven its endogenous promoter confers to races CYR32 CYR33. an ancient present wild species Aegilops longissima Ae. sharonensis ; however, it absent most cultivars, which indicates value.

Language: Английский

Citations

33
Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides DOI Creative Commons
Hongna Li, Lei Hua, Shuqing Zhao

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Sept. 28, 2023

Leaf rust, caused by Puccinia triticina Eriksson (Pt), is one of the most severe foliar diseases wheat. Breeding for leaf rust resistance a practical and sustainable method to control this devastating disease. Here, we report identification Lr47, broadly effective gene introgressed into wheat from Aegilops speltoides. Lr47 encodes coiled-coil nucleotide-binding leucine-rich repeat protein that both necessary sufficient confer Pt resistance, as demonstrated loss-of-function mutations transgenic complementation. introgression lines with no or reduced linkage drag are generated using Pairing homoeologous1 mutation, diagnostic molecular marker developed. The domain unable induce cell death, nor does it have self-protein interaction. cloning expands number genes can be incorporated multigene cassettes

Language: Английский

Citations

32