Crosstalk Between Phase-Separated Membraneless Condensates and Membrane-Bound Organelles in Cellular Function and Disease DOI

Aydan Torun,

Hoşnaz Tuğral,

Sreeparna Banerjee

et al.

Advances in experimental medicine and biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Nanocarriers address intracellular barriers for efficient drug delivery, overcoming drug resistance, subcellular targeting and controlled release DOI
Jing Liu, Horacio Cabral, Peng Mi

et al.

Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: 207, P. 115239 - 115239

Published: March 3, 2024

Language: Английский

Citations

54
Small-molecule properties define partitioning into biomolecular condensates DOI Creative Commons
Sabareesan Ambadi Thody, Hanna D. Clements, Hamid Baniasadi

et al.

Nature Chemistry, Journal Year: 2024, Volume and Issue: 16(11), P. 1794 - 1802

Published: Sept. 13, 2024

Language: Английский

Citations

38
MYC phase separation selectively modulates the transcriptome DOI
Junjiao Yang, Chan-I Chung, Jessica Koach

et al.

Nature Structural & Molecular Biology, Journal Year: 2024, Volume and Issue: 31(10), P. 1567 - 1579

Published: May 29, 2024

Language: Английский

Citations

16
Protein codes promote selective subcellular compartmentalization DOI
Henry R. Kilgore, Itamar Chinn, Peter G. Mikhael

et al.

Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

Cells have evolved mechanisms to distribute ~10 billion protein molecules subcellular compartments where diverse proteins involved in shared functions must assemble. Here, we demonstrate that with share amino acid sequence codes guide them compartment destinations. A language model, ProtGPS, was developed predicts high performance the localization of human excluded from training set. ProtGPS successfully guided generation novel sequences selectively assemble nucleolus. identified pathological mutations change this code and lead altered proteins. Our results indicate contain not only a folding code, but also previously unrecognized governing their distribution compartments.

Language: Английский

Citations

5
Structured protein domains enter the spotlight: modulators of biomolecular condensate form and function DOI Creative Commons

Nathaniel Hess,

Jerelle A. Joseph

Trends in Biochemical Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

3
Single-fluorogen imaging reveals distinct environmental and structural features of biomolecular condensates DOI Creative Commons
Tingting Wu, Matthew R. King, Yuanxin Qiu

et al.

Nature Physics, Journal Year: 2025, Volume and Issue: unknown

Published: March 14, 2025

Language: Английский

Citations

3
Ionic Effect on the Microenvironment of Biomolecular Condensates DOI

Longchen Zhu,

Yifei Pan,

Ziyi Hua

et al.

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(20), P. 14307 - 14317

Published: May 9, 2024

Biomolecules such as proteins and RNA could organize to form condensates with distinct microenvironments through liquid–liquid phase separation (LLPS). Recent works have demonstrated that the microenvironment of biomolecular plays a crucial role in mediating biological activities, partition biomolecules, subphase organization multiphasic condensates. Ions influence transition point LLPS, following Hofmeister series. However, ion-specific effect on remains unknown. In this study, we utilized fluorescence lifetime imaging microscopy (FLIM), recovery after photobleaching (FRAP), microrheology techniques investigate ion We found ions significantly affect condensates: salting-in increase micropolarity reduce microviscosity condensate, while salting-out induce opposing effects. Furthermore, manipulate miscibility multilayering behavior summary, our work provides first quantitative survey protein presence from series, demonstrating how impact micropolarity, microviscosity, viscoelasticity Our results bear implications membrane-less organelles would exhibit varying continuously changing cellular conditions.

Language: Английский

Citations

15
Biomolecular condensates can function as inherent catalysts DOI

Xiao Guo,

Mina Farag,

Naixin Qian

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 10, 2024

We report the discovery that chemical reactions such as ATP hydrolysis can be catalyzed by condensates formed intrinsically disordered proteins (IDPs), which themselves lack any intrinsic ability to function enzymes. This inherent catalytic feature of derives from electrochemical environments and electric fields at interfaces are direct consequences phase separation. The we studied were capable catalyzing diverse reactions, including radical-dependent breakdown whereby fully decomposes adenine multiple carbohydrates. distinguishes naturally occurring ATPases, only catalyze dephosphorylation ATP. Interphase interfacial properties tuned via sequence design, thus enabling control over catalysis through sequence-dependent features condensates. Incorporation hydrolase-like synthetic into live cells enables activation transcriptional circuits depend on products reactions. Inherent functions condensates, emergent separation, likely affect metabolic regulation in cells.

Language: Английский

Citations

12
Biomolecular condensates in immune cell fate DOI
Srikanth Kodali, Caroline M. Sands, Lei Guo

et al.

Nature reviews. Immunology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Language: Английский

Citations

1
Aging-dependent evolving electrochemical potentials of biomolecular condensates regulate their physicochemical activities DOI
Wen Yu,

Xiao Guo,

Yu Xia

et al.

Nature Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Language: Английский

Citations

1