Identification of Transcriptional Regulators of Immune Evasion Across Cancers: An Alternative Immunotherapeutic Strategy for Cholangiocarcinoma DOI Open Access
Simran Venkatraman, Brinda Balasubramanian, Pornparn Kongpracha

et al.

Cancers, Journal Year: 2024, Volume and Issue: 16(24), P. 4197 - 4197

Published: Dec. 17, 2024

Background: Cancer immune evasion is a multifaceted process that synchronizes pro-tumoral infiltration, immunosuppressive inflammation, and inhibitory checkpoint expression (IC). Current immunotherapies combat this issue by reinstating immunosurveillance of tumors; however, it benefits limited patient population. Thus, more effective immunotherapeutic strategy warranted to cater specific populations. This investigation introduces novel via inhibition master regulators (MR-IE). Methods: Samples the TCGA Pan-Cancer Atlas transcriptomic data were subset stratified based on IC estimated cell infiltration. Transcriptomic analysis was conducted unravel pathways associated with process. Transcription factor enrichment survival analyses identify rank candidate MR-IEs per cancer type. Results: Inhibition top-ranking MR-IE cholangiocarcinoma (CCA), MYC, modulated gene protein PD-L1. Moreover, inflammatory markers, IFNA21 CX3CL1, downregulated, anti-tumoral cytokines, IL-18 IL-16, upregulated. Lastly, MYC potentiated fourth-generation anti-folate receptor alpha (FRα) CAR-T therapy against CCA cells. Conclusions: Cumulatively, study highlights promise as potent for treatment offers list type further validation.

Language: Английский

GNGT1 remodels the tumor microenvironment and promotes immune escape through enhancing tumor stemness and modulating the fibrinogen beta chain-neutrophil extracellular trap signaling axis in lung adenocarcinoma DOI Open Access

Linlin Fan,

Xiaowei Wang, Xiu‐Mei Zhang

et al.

Translational Lung Cancer Research, Journal Year: 2025, Volume and Issue: 14(1), P. 239 - 259

Published: Jan. 1, 2025

Despite the recent advancements in treatment of cancer, 5-year survival patients with non-small cell lung cancer (NSCLC) remains unsatisfactory. Lung adenocarcinoma (LUAD) is NSCLC's most common subtype, and metastasis major cause death cancer. Therefore, identifying novel targets associated NSCLC crucial to improving treatment. This study aimed characterize expression GNGT1 LUAD clarify mechanism underlying association between higher level worse prognosis patients. The transcriptome datasets clinical information were obtained from Cancer Genome Atlas (TCGA) Gene Expression Omnibus (GEO) database. Bioinformatics analyses performed 515 who stratified into two groups (high- low-GNGT1 group) according level. Overall survival, DNA promotor methylation, immune infiltration, gene set enrichment analysis (GSEA), Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway elucidate functions identify related hub genes LUAD. Their verified using tissues transgenic mice overexpressing under control a lung-specific promoter (Scgb1a1-Cre). was overexpressed poor prognosis. significantly correlated alteration hypomethylated status. High advanced lymph node degree infiltration. Functional indicated that differentially expressed (DEGs) high-GNGT1 group participated replication, replication preinitiation, M phase, while adhesion molecules, apoptosis, natural killer cell-mediated cytotoxicity all downregulated. Messenger RNA protein levels correspondingly regulated human Scgb1a1-Cre; LSL-GNGT1 mouse model (GNGT1fl/+ mice). tumor proliferation via enhancement stemness interaction driver genes. Elevated promoted epithelial-mesenchymal transformation, remodeled microenvironment, led metastasis, ultimately worsening survival-related

Language: Английский

Citations

0
Single-cell profiling of SLC family transporters: uncovering the role of SLC7A1 in osteosarcoma DOI Creative Commons

Yan Liao,

Junkai Chen, Hao Yao

et al.

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 22, 2025

Osteosarcoma is the most common malignant bone tumor in children and adolescents, characterized by high disability mortality rates. Over past three decades, therapeutic outcomes have plateaued, underscoring critical need for innovative targets. Solute carrier (SLC) family transporters been implicated progression of a variety tumors, however, their specific role osteosarcoma remains poorly understood. The single-cell sequencing data from GSE152048 GSE162454, along with RNA-seq TARGET GSE21257 cohorts, were utilized analysis this study. LASSO regression was conducted to identify prognostic genes construct an SLC-related signature. Survival ROC evaluated validity ESTIMATE CIBERSORT Packages assess immune infiltration status. Pseudotime CellChat analyses performed investigate relationship between SLC7A1, phenotypes, microenvironment. CCK8 assays, EdU staining, colony formation Transwell co-culture systems used effects SLC7A1 on cell proliferation, metastasis, macrophage polarization. Finally, virtual docking identified potential drugs targeting SLC7A1. SLCs displayed distinct expression patterns across various types within microenvironment, myeloid cells exhibiting preference amino acid uptake. A model comprising nine constructed via regression, showing highest hazard ratio. Multiple analytical algorithms indicated that associated checkpoint gene expression. Single-cell predominantly expressed correlated characteristics. also regulate interactions macrophages, as well modulate function through multiple pathways. In vitro assays survival demonstrated inhibition suppressed phenotype cells, correlating poor prognosis. Co-culture models confirmed involvement screening CETSA Cepharanthine inhibitors signatures can be evaluation osteosarcoma. Pharmacological may feasible approach

Language: Английский

Citations

0
Drug Delivery Systems Based on Metal–Organic Frameworks for Tumor Immunotherapy DOI Creative Commons
Ning Yang,

Zongyan He,

Tianqun Lang

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(2), P. 225 - 225

Published: Feb. 10, 2025

Metal–organic frameworks (MOFs) are a class of inorganic-organic hybrid nanoparticles formed by the coordination metal ions/clusters and organic ligands. Due to their high porosities, large surface areas, adjustable structures, responsiveness light/sound, etc., MOFs have shown great clinical potential in field tumor therapy. Tumor immunotherapy exerts antitumor effects through reshaping immune microenvironment, showing significant preclinical advantages. Based on mechanisms immunity activation, agents can be divided into chemotherapeutic agents, immunomodulators, enzymes, vaccines oligonucleotide drugs, etc. Herein, we review MOFs-based drug delivery systems for immunotherapy. The classification MOFs, followed activation mechanisms, first introduced. Drug based with different also summarized, especially synergetic triggered loadings. Furthermore, merits drawbacks strategies promote applications discussed.

Language: Английский

Citations

0
Liquid-liquid phase separation drives immune signaling transduction in cancer: a bibliometric and visualized study from 1992 to 2024 DOI Creative Commons
Y. P. Pei, Haijie Liang, Yuming Guo

et al.

Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 15

Published: March 4, 2025

Liquid-liquid phase separation (LLPS) is a novel concept that could explain how living cells precisely modulate internal spatial and temporal functions. However, comprehensive bibliometric analysis on LLPS immune signaling processes in cancer still scarce. This study aims to perform assessment of research explore the landscape pathways for cancer. Utilizing Web Science Core Collection database multiple software, we performed quantitative qualitative analyses situation between from 1992 2024. The corresponding authors were primarily China USA. most relevant references "International Journal Molecular Sciences", "Proteomics". annual number publications exhibited fast upward tendency 2020 frequent key terms included expression, separation, activation, immunotherapy, mechanisms. Qualitative evaluation emphasized TCR, BCR, cGAS-STING, RIG-1, NF-κB associated with processes. first integratively map out knowledge structure forward direction area transduction linked over past 30 years. In summary, although this its infancy, illustrating coordinated structures communications within framework will offer deeper insights into molecular mechanisms development further enhance effectiveness existing immunotherapies.

Language: Английский

Citations

0
Insulin resistance and cancer: molecular links and clinical perspectives DOI
Alfredo Caturano, Enes Erul,

Roberto Nilo

et al.

Molecular and Cellular Biochemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 15, 2025

Language: Английский

Citations

0
Molecular Strategies for Constructing Epoxy‐Type Afterglow Probes in Disease Diagnosis and Treatment DOI

Maocheng Zuo,

Yidan Hu,

L. Xiao

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

Abstract Epoxy‐type afterglow luminescence is a cascade reaction of photochemical defects caused as result the formation and release epoxy intermediates, exhibiting continuous phenomenon that remains intrinsic after excitation, with extremely impressive signal‐to‐background ratio (SBR) level in vivo. Afterglow based on this epoxy‐type mechanism ideally suited for applications developments diagnosis treatment biological diseases. In tutorial review, work aims to deeply elaborate mechanisms materials present application imaging Lastly, future perspectives potential challenges technology preclinical diagnostic, therapeutic, predictive analyses, well clinical translations are discussed.

Language: Английский

Citations

0
A STAT3–STING–IFN axis controls the metastatic spread of small cell lung cancer DOI

Aleks C. Guanizo,

Quinton Luong,

W. Samantha N. Jayasekara

et al.

Nature Immunology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 21, 2024

Language: Английский

Citations

3
Personalized nanovaccines for treating solid cancer metastases DOI Creative Commons
Feng Tang, Jia Hu,

Jirui Wen

et al.

Journal of Hematology & Oncology, Journal Year: 2024, Volume and Issue: 17(1)

Published: Nov. 28, 2024

Cancer vaccines have garnered attention as a potential treatment for cancer metastases. Nevertheless, the clinical response rate to remains < 30%. Nanoparticles stabilize and improve antigen recognition presentation, resulting in high tumor penetration or accumulation, effective co-distribution of drugs secondary lymphatic system, adaptable adjuvant administration. Such vaccine-like nanomedicines ability eradicate primary tumors well prevent eliminate This review examines state-of-the-art nanocarriers developed deliver metastases, including synthetic, semi-biogenic, biogenic nanosystems. Moreover, it highlights physical pharmacological properties that enhance their anti-metastasis efficiency. also addresses combination nanovaccines with immunotherapy target various steps metastatic cascade, drawing insights from preclinical studies. The concludes critical analysis challenges frameworks linked translation nanovaccines.

Language: Английский

Citations

2
Withaferin-A induced Vimentin S56 phosphorylation dissociates NEDD9 signaling loop to regress progressive metastatic melanoma into lung adenocarcinoma. DOI

Ankith Sherapura,

Bayram Kıran,

G.S. Pavan Kumar

et al.

Chemico-Biological Interactions, Journal Year: 2024, Volume and Issue: unknown, P. 111319 - 111319

Published: Nov. 1, 2024

Language: Английский

Citations

1
Identification of Transcriptional Regulators of Immune Evasion Across Cancers: An Alternative Immunotherapeutic Strategy for Cholangiocarcinoma DOI Open Access
Simran Venkatraman, Brinda Balasubramanian, Pornparn Kongpracha

et al.

Cancers, Journal Year: 2024, Volume and Issue: 16(24), P. 4197 - 4197

Published: Dec. 17, 2024

Background: Cancer immune evasion is a multifaceted process that synchronizes pro-tumoral infiltration, immunosuppressive inflammation, and inhibitory checkpoint expression (IC). Current immunotherapies combat this issue by reinstating immunosurveillance of tumors; however, it benefits limited patient population. Thus, more effective immunotherapeutic strategy warranted to cater specific populations. This investigation introduces novel via inhibition master regulators (MR-IE). Methods: Samples the TCGA Pan-Cancer Atlas transcriptomic data were subset stratified based on IC estimated cell infiltration. Transcriptomic analysis was conducted unravel pathways associated with process. Transcription factor enrichment survival analyses identify rank candidate MR-IEs per cancer type. Results: Inhibition top-ranking MR-IE cholangiocarcinoma (CCA), MYC, modulated gene protein PD-L1. Moreover, inflammatory markers, IFNA21 CX3CL1, downregulated, anti-tumoral cytokines, IL-18 IL-16, upregulated. Lastly, MYC potentiated fourth-generation anti-folate receptor alpha (FRα) CAR-T therapy against CCA cells. Conclusions: Cumulatively, study highlights promise as potent for treatment offers list type further validation.

Language: Английский

Citations

0