New mouse model for inducible hACE2 expression enables to dissect SARS-CoV-2 pathology beyond the respiratory system DOI Creative Commons

Federica Gambini,

Dominik Arbon, Petr Nickl

et al.

Mammalian Genome, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 22, 2025

The Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection is not limited to the respiratory tract as receptors, including angiotensin-converting enzyme 2 (ACE2), are expressed across many tissues. This study employed a new conditional mouse model, Rosa26creERT2/chACE2, which expresses human ACE2 (hACE2) multiple organs, investigate effects of SARS-CoV-2 beyond system. strain demonstrated susceptibility in dose and sex-dependent manner, showing that infected male mice exhibited more severe disease outcomes, significant weight loss, pronounced lung pathology dysfunction, increased mortality, compared females. In contrast intratracheal infection, intranasal virus administration facilitated viral spread brain, thereby underscoring nasal route's role pathogenesis neurological manifestations. Intranasal also led innate immune system activation administration, even though both routes activated adaptive response. model provides valuable tool individual tissues or use multisystemic approach, it advances possibilities for preclinical evaluation antiviral therapies vaccine strategies.

Language: Английский

Distinct CD8+ T-cell types Associated with COVID-19 Severity in Unvaccinated HLA-A2+ Patients DOI Creative Commons
Kazuya Masuda, Sho Iketani, Lihong Liu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 14, 2025

Abstract Although emerging data have revealed the critical role of memory CD8 + T cells in preventing and controlling SARS-CoV-2 infection, virus-specific T-cell responses against its innate-like subsets unvaccinated COVID-19 patients with various disease manifestations an HLA-restricted fashion remain to be understood. Here, we show strong association protective cellular immunity mild unique cell types virus HLA-A2 restricted manner. ELISpot assays reveal that SARS-CoV-2-specific are significantly higher than severe patients, whereas neutralizing antibody correlate severity. Single-cell analyses HLA-A2-restricted cells, which recognize highly conserved immunodominant epitopes, demonstrate divergent profiles versus disease. including cytotoxic KLRB1 CD8αα signatures, IFNG hi ID3 IL7R proliferative stem cell-like preferentially observed COVID-19, distinct terminally-differentiated predominantly detected COVID-19: activated FASL early-terminated or dysfunctional IL4R GATA3 subset. In conclusion, our findings suggest contrasting may dictate Figure Graphical abstract. epitope-specific subtypes associated patients. Potent gene signature (upper) (lower).

Language: Английский

Citations

0
Post COVID-19 and Long COVID Symptoms in Otorhinolaryngology—A Narrative Review DOI Open Access
Orlando Guntinas–Lichius,

Thomas Bitter,

Robert P. Takes

et al.

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(2), P. 506 - 506

Published: Jan. 14, 2025

Post/Long COVID (syndrome) is defined as a condition with symptoms persisting for more than 12 weeks after the onset of SARS-CoV-2 infection that cannot be explained otherwise. The prevalence self-reported otorhinolaryngological high. aim this review was to analyze current literature regarding actual prevalence, knowledge etiopathology, and evidence-based treatment recommendations otorhinolaryngology-related symptoms. A systematic search articles published since 2019 in PubMed ScienceDirect performed resulted 108 articles. These were basis formed comprehensive series consented therapy statements on most important Otorhinolaryngological did not appear isolated but part multi-organ syndrome. Self-reported often confirmed by objective testing. estimated anosmia, dysgeusia, cough, facial palsy, hoarseness/dysphonia, acute hearing loss, tinnitus, vertigo/dizziness about 4%, 2%, 4-19%, 0%, 17-20%, 8%, 20%, 5-26%, respectively. There are manifold theoretical concepts etiopathology different symptoms, there no clear proof. This certainly contributes fact effective specific option any mentioned. Healthcare pathways must established so can recognized evaluated otorhinolaryngologists provide counseling. would also help establish selectively include patients clinical trials investigating therapeutic concepts.

Language: Английский

Citations

0
Animal Models of Non-Respiratory, Post-Acute Sequelae of COVID-19 DOI Creative Commons
Abigail Vanderheiden, Michael Diamond

Viruses, Journal Year: 2025, Volume and Issue: 17(1), P. 98 - 98

Published: Jan. 14, 2025

Post-acute sequelae of COVID-19 (PASC) are a diverse set symptoms and syndromes driven by dysfunction multiple organ systems that can persist for years negatively impact the quality life millions individuals. We currently lack specific therapeutics patients with PASC, due in part to an incomplete understanding its pathogenesis, especially non-pulmonary sequelae. Here, we discuss three animal models have been utilized investigate PASC: non-human primates (NHPs), hamsters, mice. focus on neurological, gastrointestinal, cardiovascular PASC highlight advances mechanistic insight made using these models, as well discussing warrant continued intensive research.

Language: Английский

Citations

0
Post infectious fatigue and circadian rhythm disruption in long-COVID and other infections: a need for further research DOI Creative Commons

Achilleas Livieratos,

Steven W. Lockley, Sotirios Tsiodras

et al.

EClinicalMedicine, Journal Year: 2025, Volume and Issue: 80, P. 103073 - 103073

Published: Jan. 18, 2025

Language: Английский

Citations

0
Glial activation among individuals with Neurological Post-Acute Sequelae of Coronavirus disease 2019: A Brain Fog Positron Emission Tomography Study of [18F]-FEPPA DOI Creative Commons
Sean Clouston, P. Vaska, Tesleem Kayode Babalola

et al.

Brain Behavior & Immunity - Health, Journal Year: 2025, Volume and Issue: 44, P. 100945 - 100945

Published: Jan. 18, 2025

Language: Английский

Citations

0
Altered immune surveillance of B and T cells in patients with persistent residual lung abnormalities 12 months after severe COVID-19 DOI Creative Commons
Julio Flores‐González, Ivette Buendia‐Roldán,

Fernanda Téllez-Quijada

et al.

Respiratory Research, Journal Year: 2025, Volume and Issue: 26(1)

Published: Jan. 18, 2025

Post-COVID-19 respiratory sequelae often involve lung damage, which is called residual abnormalities, and potentially lead to chronic issues. The adaptive immune response, involving T-cells B-cells, plays a critical role in pathogen control, inflammation, tissue repair. However, the link between dysregulation development of abnormalities remains unclear. 109 patients discharged with after COVID-19 were followed for 12 months divided as full recovery (FRG, n = 88) persistent (PLAG, 21). Cell profiling analysis was done using flow cytometry at 24 h not antigen-specific vitro stimulation. Plasma or supernatant levels IFN-g, IL-4, IL-10, IgM, IgG assessed, 10 (5 FRG, 5 PLAG) randomly selected detailed cell phenotyping functional peripheral blood mononuclear cells cytometry. Compared FRG group, PLAG exhibited an increase unswitched (p 0.0159) decreased double-negative activated B-cells 0.0317), systemic IL-10 lower, displayed reduced frequency total impaired spontaneous IgM 0.0357) 0.0079) release culture. Regarding T-cells, showed reduction effector memory CD4 + TEMRA following Notably, group also higher frequencies central Th2 (GATA3+) response activation than 0.0079). Patients post-critical exhibit B-cell function, increased activation. These imbalances may contribute ongoing dysfunction warrant further investigation potential mechanism abnormalities. Larger studies are necessary confirm these findings.

Language: Английский

Citations

0
Analysis of related factors for systemic lupus erythematosus flare after SARS‐Cov‐2 infection: A retrospective cohort study DOI
Qian Li, Junkang Zhao, Junyan Zhang

et al.

International Journal of Rheumatic Diseases, Journal Year: 2025, Volume and Issue: 28(1)

Published: Jan. 1, 2025

Abstract Objective Since COVID‐19 infections are more common in systemic lupus erythematosus (SLE) patients, most recent research has focused on the outcome of COVID‐19, with fewer studies disease activity SLE. This aims to evaluate flares SLE infection while investigating predictive factors. Methods A questionnaire survey was conducted collect information patients previously diagnosed from multi‐center. infected after December 7, 2022, were selected. Detailed covering demographic characteristics, and clinical features, activity, medication collected through an electronic questionnaire. multivariate logistic regression model constructed factors for onset infection. Results total 240 finally included our analysis. Thirty (12.5%) those enrolled reported flare. Multivariate analysis models confirmed that active stage (OR 2.617, 95% CI 1.008–6.514, p = .041) duration 4.140, 1.412–11.694, .008) predictors flare Covid‐19 In contrast, immunosuppressants associated a low incidence 0.138, 0.042–0.46, .005). Conclusions The phase progression main risk exacerbation use immunosuppressive medications lower flare‐ups. These findings provide valuable insights managing during pandemic.

Language: Английский

Citations

0
From dyspnea to Olympic qualification - a case report as a model for post-acute sequelae of COVID-19 DOI Creative Commons

Baudrexl Jc,

K Steinestel,

D Gagiannis

et al.

Heliyon, Journal Year: 2025, Volume and Issue: 11(2), P. e42195 - e42195

Published: Jan. 1, 2025

Pulmonary post-acute COVID-19 sequelae (PASC) can occur in professional athletes. In these individuals, the physical impairment ultimately mean end of their career due to lack specific treatment strategies. Individualised approaches are required, as demonstrated this case report. Using example a female athlete, an individualised therapy strategy is outlined step by on basis assessment lung function, including spiroergometry, CT imaging and immunocytological histopathological analysis bronchoscopically obtained material. This multimodal diagnostic approach was used identify persistent inflammation without viral persistence create individual plan. addition use azithromycin anti-obstructive inhalation therapy, patient also received three sessions thermoplasty new physiotherapy (myoreflex therapy). gradually freed athlete from her restrictions. Over time, she able regain athletic performance, which led successful qualification for 2024 Summer Olympics Paris. All objective examination parameters indicated remission pulmonary PASC. To date, there no clear recommendations The most commonly prescribed regimens often fail achieve success. present highlights need therapeutic that complete PASC be achieved. Based our findings, multidisciplinary phenotyping cases appears central importance approach. It should emphasised inflammatory processes persist persistence.

Language: Английский

Citations

0
Wirksamkeit Kneipp’scher Kniegüsse auf die Fatigue-Symptomatik des Post-COVID-Syndroms – eine randomisierte kontrollierte Pilotstudie DOI

R. Küchler,

Norman Best, Steffen Derlien

et al.

Physikalische Medizin Rehabilitationsmedizin Kurortmedizin, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

Zusammenfassung Für das vorherrschende Symptom Fatigue im Rahmen des Post-COVID-Syndroms (PCS) existieren weiterhin nur eingeschränkte Behandlungsstrategien. Hydrotherapeutische Verfahren zeigten bei anderen Ursachen der positive Effekte. Das primäre Ziel ist es erstmals objektivierbare Daten mittels HRV zur Wirksamkeit von Kniegüssen nach Kneipp auf die Fatique Post-COVID zu generieren. Personen mit relevanter Fatigue, gemessen Chalder Skala, wurden in Studie eingeschlossen. Die Herzratenvariablitität (HRV) (Zeit und Frequenz-Domänen) sowie Lebensqualität (SF-12) erfasst. Es erfolgte eine Randomisierung Interventionsgruppe (IG) Kontrollgruppe (KG). IG verwendete zwei- bis dreimal pro Woche kaltes Wasser für Kniegüsse, während KG warmes verwendete. Nach sechswöchiger eigenständiger Durchführung erneut erhoben Mittelwertvergleichen undRegressionsmodellen analysiert. Insgesamt 30 Betroffene eingeschlossen, denen 80% angaben, weiblich sein. Ausprägung verbesserten sich allen Teilnehmenden über Zeit. konnte kein signifikanter Unterschied zwischen den HRV-Werten Gruppen gefunden werden (p>0,05). Auch Auswertung weiteren Ourctomeparameter ergab keine Unterschiede Gruppen. Kniegüsse können einen Einfluss beim PCS haben, jedoch fraglich, ob Knieguss seiner relativ geringen Reizfläche ausreichend ist, um relevante Änderung Symptome erreichen. Ein deutlicher warmen kalten Gussgruppe ließ nicht ermitteln, was auch kleinen Stichprobe begründet sein könnte. Weitere Studien optimierten Studienbedingungen einer größeren Kohorte sind notwendig.

Citations

0
Positionspapier zum Post-COVID-Syndrom DOI

Elisabeth Schieffer,

Denise Hilfiker‐Kleiner, Axel Schlitt

et al.

Deleted Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Citations

0