New mouse model for inducible hACE2 expression enables to dissect SARS-CoV-2 pathology beyond the respiratory system DOI Creative Commons

Federica Gambini,

Dominik Arbon, Petr Nickl

et al.

Mammalian Genome, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 22, 2025

The Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection is not limited to the respiratory tract as receptors, including angiotensin-converting enzyme 2 (ACE2), are expressed across many tissues. This study employed a new conditional mouse model, Rosa26creERT2/chACE2, which expresses human ACE2 (hACE2) multiple organs, investigate effects of SARS-CoV-2 beyond system. strain demonstrated susceptibility in dose and sex-dependent manner, showing that infected male mice exhibited more severe disease outcomes, significant weight loss, pronounced lung pathology dysfunction, increased mortality, compared females. In contrast intratracheal infection, intranasal virus administration facilitated viral spread brain, thereby underscoring nasal route's role pathogenesis neurological manifestations. Intranasal also led innate immune system activation administration, even though both routes activated adaptive response. model provides valuable tool individual tissues or use multisystemic approach, it advances possibilities for preclinical evaluation antiviral therapies vaccine strategies.

Language: Английский

Hyperthermia and targeting heat shock proteins: innovative approaches for neurodegenerative disorders and Long COVID DOI Creative Commons
David M. Smadja,

M. Marc Abreu

Frontiers in Neuroscience, Journal Year: 2025, Volume and Issue: 19

Published: Feb. 4, 2025

Neurodegenerative diseases (NDs) and Long COVID represent critical growing global health challenges, characterized by complex pathophysiological mechanisms including neuronal deterioration, protein misfolding, persistent neuroinflammation. The emergence of innovative therapeutic approaches, such as whole-body hyperthermia (WBH), offers promising potential to modulate underlying in NDs related conditions like COVID. WBH, particularly fever-range, enhances mitochondrial function, induces heat shock proteins (HSPs), modulates neuroinflammation—benefits that pharmacological treatments often struggle replicate. HSPs HSP70 HSP90 play pivotal roles folding, aggregation prevention, cellular protection, directly targeting pathological processes seen Alzheimer's, Parkinson's, Huntington's disease. Preliminary findings also suggest WBH's alleviate neurological symptoms COVID, where neuroinflammation serotonin dysregulation are prominent. Despite the absence robust clinical trials, implications WBH extend immune modulation restoration disrupted physiological pathways. However, dual nature hyperthermia's effects—balancing pro-inflammatory anti-inflammatory responses—emphasizes need for dose-controlled applications stringent patient monitoring minimize risks vulnerable populations. While shows interest, significant challenges remain. These include individual variability response, limited accessibility advanced technologies, standardized protocols. Future research must focus on targeted biomarker identification, personalized treatment strategies optimize efficacy integration into paradigms could mark a transformative step addressing these conditions.

Language: Английский

Citations

0
Healthcare utilization patterns before and after a long COVID diagnosis: a case-control study DOI Creative Commons

Rick DeVoss,

Elizabeth J. Carlton, Sarah E. Jolley

et al.

BMC Public Health, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 10, 2025

Documenting Long COVID cases has been challenging partly due to the lack of population-level data and uncertain diagnostic criteria, hindering ability ascertain healthcare utilization patterns over time. The objective this study is examine characteristics patients in Colorado pre- post-diagnosis compared controls. Retrospective, longitudinal case-control using a 100% sample Colorado's All-Payer Claims Database. includes individuals 18 or older diagnosed with between October 1, 2021, August 2022, followed until 2023. was identified International Classification Diseases, 10th Revision, U09.9 code medical insurance claims. Analysis required one year continuous enrollment before after diagnosis. Controls were matched 2:1 on age group, sex, payer, index month account for contemporaneous trends utilization. 26,358 ever COVID, resulting claims-based prevalence 674 per 100,000 during period (population 3,906,402 individuals). Of these, 12,698 had diagnosis: mean (SD) age, 59.0 (17.1); 65.3% female; 60.1% white; 83.0% residing urban areas. 25,376 Before diagnosis, 17% hospitalized at least once, 40% visited an emergency department occasion. Within following acute services significantly decreased relative controls: hospitalizations, -6.1percentage points (p.p.), visits, -7.7 p.p., whereas outpatient medications increased: office 3.6 p.p.; specialist 4.7 5.2 new medications, (controls: 2.8). Changes diagnoses some conditions (e.g., metastatic carcinomas lung cancer) similar groups. increased switching from care settings. change service settings among population suggests that diagnosis could lead better patient management. Healthcare these high, underscoring need understand burden systems data.

Language: Английский

Citations

0
Post-Recovery Mental Health Outcomes in Young Adults with Mild-to-Moderate COVID-19: A Cross-Sectional Study DOI Creative Commons
Ashkan Latifi

COVID, Journal Year: 2025, Volume and Issue: 5(2), P. 27 - 27

Published: Feb. 19, 2025

Background: The long-term effects of COVID-19 infection represent an emerging area research that explores the relationships between a history and its consequential sequelae. This study investigates potential associations among time since infection, severity acute phase disease, sex, while controlling for age, in relation to mental health. Methods: A total 305 university students participated this cross-sectional study, during which data were collected using SCL-90-R questionnaire. analysis was conducted MANCOVA, ANCOVA, partial Kendall’s Tau methods. Results: findings indicated factors such as sex—specifically being female—longer elapsed disease significantly influenced multiple scales SCL-90-R. Conclusions: Based on these findings, it is recommended investigations into health issues consider biological severity, risk young adults with infection.

Language: Английский

Citations

0
Long COVID Psychiatric Sequelae, Biochemical Markers & Tau Protein: A 3-Year Follow-Up Study DOI
Shivani Jaiswal, Swapnajeet Sahoo, Vandana Dhiman

et al.

Psychiatry Research, Journal Year: 2025, Volume and Issue: 347, P. 116413 - 116413

Published: Feb. 19, 2025

Language: Английский

Citations

0
New mouse model for inducible hACE2 expression enables to dissect SARS-CoV-2 pathology beyond the respiratory system DOI Creative Commons

Federica Gambini,

Dominik Arbon, Petr Nickl

et al.

Mammalian Genome, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 22, 2025

The Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection is not limited to the respiratory tract as receptors, including angiotensin-converting enzyme 2 (ACE2), are expressed across many tissues. This study employed a new conditional mouse model, Rosa26creERT2/chACE2, which expresses human ACE2 (hACE2) multiple organs, investigate effects of SARS-CoV-2 beyond system. strain demonstrated susceptibility in dose and sex-dependent manner, showing that infected male mice exhibited more severe disease outcomes, significant weight loss, pronounced lung pathology dysfunction, increased mortality, compared females. In contrast intratracheal infection, intranasal virus administration facilitated viral spread brain, thereby underscoring nasal route's role pathogenesis neurological manifestations. Intranasal also led innate immune system activation administration, even though both routes activated adaptive response. model provides valuable tool individual tissues or use multisystemic approach, it advances possibilities for preclinical evaluation antiviral therapies vaccine strategies.

Language: Английский

Citations

0