Increased expression of schizophrenia-associated gene C4 leads to hypoconnectivity of prefrontal cortex and reduced social interaction DOI Creative Commons
Ashley L. Comer, Tushare Jinadasa, Balaji Sriram

et al.

PLoS Biology, Journal Year: 2020, Volume and Issue: 18(1), P. e3000604 - e3000604

Published: Jan. 14, 2020

Schizophrenia is a severe mental disorder with an unclear pathophysiology. Increased expression of the immune gene C4 has been linked to greater risk developing schizophrenia; however, it not known whether plays causative role in this brain disorder. Using confocal imaging and whole-cell electrophysiology, we demonstrate that overexpression mouse prefrontal cortex neurons leads perturbations dendritic spine development hypoconnectivity, which mirror neuropathologies found schizophrenia patients. We find evidence microglia-mediated synaptic engulfment enhanced increased C4. also show C4-dependent circuit dysfunction frontal decreased social interactions juvenile adult mice. These results schizophrenia-associated causes aberrant wiring deficits behavior, suggesting altered contributes directly pathogenesis.

Language: Английский

3D printing of hydrogels: Rational design strategies and emerging biomedical applications DOI Creative Commons
Jinhua Li, Chengtie Wu, Paul K. Chu

et al.

Materials Science and Engineering R Reports, Journal Year: 2020, Volume and Issue: 140, P. 100543 - 100543

Published: Feb. 18, 2020

3D printing alias additive manufacturing can transform virtual models created by computer-aided design (CAD) into physical objects in a layer-by-layer manner dispensing with conventional molding or machining. Since the incipiency, significant advancements have been achieved understanding process of and relationship component, structure, property application objects. Because hydrogels are one most feasible classes ink materials for this field has rapidly advancing, Review focuses on hydrogel designs development advanced hydrogel-based biomaterial inks bioinks printing. It covers techniques including laser (stereolithography, two-photon polymerization), extrusion (3D plotting, direct writing), inkjet printing, bioprinting, 4D bioprinting. provides comprehensive overview discussion tailorability material, mechanical, physical, chemical biological properties to enable The range hydrogel-forming polymers covered encompasses biopolymers, synthetic polymers, polymer blends, nanocomposites, functional cell-laden systems. representative biomedical applications selected demonstrate how is being exploited tissue engineering, regenerative medicine, cancer research, vitro disease modeling, high-throughput drug screening, surgical preparation, soft robotics flexible wearable electronics. Incomparable thermoplastics, thermosets, ceramics metals, playing pivotal role creation (bio)systems customizable way. An outlook future directions presented.

Language: Английский

Citations

693
CODEX multiplexed tissue imaging with DNA-conjugated antibodies DOI
Sarah Black, Darci J. Phillips, John W. Hickey

et al.

Nature Protocols, Journal Year: 2021, Volume and Issue: 16(8), P. 3802 - 3835

Published: July 2, 2021

Language: Английский

Citations

441
Expansion sequencing: Spatially precise in situ transcriptomics in intact biological systems DOI
Shahar Alon, Daniel Goodwin,

Anubhav Sinha

et al.

Science, Journal Year: 2021, Volume and Issue: 371(6528)

Published: Jan. 28, 2021

Identifying transcript location in cells where specific RNAs occur within a cell or tissue has been limited by technology and imaging capabilities. Expansion microscopy allowed for better visualization of small structures expanding the tissues with polymer- hydrogel-based system. Alon et al. combined expansion long-read situ RNA sequencing, resulting more precise transcripts. This method, termed “ExSeq” was used to detect RNAs, both new transcripts those previously demonstrated localize neuronal dendrites. Unlike other sequencing methods, ExSeq does not target sets genes. thus unites spatial resolution, multiplexing, an unbiased approach reveal insights into localization its physiological roles developing active tissue. Science , this issue p. eaax2656

Language: Английский

Citations

319
Understanding 3D genome organization by multidisciplinary methods DOI
Ivana Jerković, Giacomo Cavalli

Nature Reviews Molecular Cell Biology, Journal Year: 2021, Volume and Issue: 22(8), P. 511 - 528

Published: May 5, 2021

Language: Английский

Citations

303
Synaptic Specificity, Recognition Molecules, and Assembly of Neural Circuits DOI Creative Commons
Joshua R. Sanes, S Lawrence Zipursky

Cell, Journal Year: 2020, Volume and Issue: 181(3), P. 536 - 556

Published: April 1, 2020

Language: Английский

Citations

294
Could 3D models of cancer enhance drug screening? DOI
Virginia Brancato, Joaquím M. Oliveira, Vítor M. Correlo

et al.

Biomaterials, Journal Year: 2019, Volume and Issue: 232, P. 119744 - 119744

Published: Dec. 26, 2019

Language: Английский

Citations

228
Complexities of the glomerular basement membrane DOI
Richard W. Naylor, Mychel Morais, Rachel Lennon

et al.

Nature Reviews Nephrology, Journal Year: 2020, Volume and Issue: 17(2), P. 112 - 127

Published: Aug. 24, 2020

Language: Английский

Citations

160
Super-Resolution Microscopy for Structural Cell Biology DOI
Sheng Liu, Philipp Hoess, Jonas Ries

et al.

Annual Review of Biophysics, Journal Year: 2022, Volume and Issue: 51(1), P. 301 - 326

Published: Feb. 4, 2022

Super-resolution microscopy techniques, and specifically single-molecule localization (SMLM), are approaching nanometer resolution inside cells thus have great potential to complement structural biology techniques such as electron for cell biology. In this review, we introduce the different flavors of super-resolution microscopy, with a special emphasis on SMLM MINFLUX (minimal photon flux). We summarize recent technical developments that pushed these localization-based scales review experimental conditions key obtaining data highest quality. Furthermore, give an overview analysis methods highlight studies used gain insights into biologically relevant molecular machines. Ultimately, our perspective what is needed push even further apply them investigating dynamic rearrangements in living cells.

Language: Английский

Citations

146
Molecular resolution imaging by post-labeling expansion single-molecule localization microscopy (Ex-SMLM) DOI Creative Commons

Fabian U. Zwettler,

Sebastian Reinhard, Davide Gambarotto

et al.

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: July 7, 2020

Abstract Expansion microscopy (ExM) enables super-resolution fluorescence imaging of physically expanded biological samples with conventional microscopes. By combining ExM single-molecule localization (SMLM) it is potentially possible to approach the resolution electron microscopy. However, current attempts combine both methods remained challenging because protein and fluorophore loss during digestion or denaturation, gelation, incompatibility polyelectrolyte hydrogels photoswitching buffers. Here we show that re-embedding d STORM demonstrate post-labeling resolves limitations Using microtubules as a reference structure centrioles, Ex-SMLM preserves ultrastructural details, improves labeling efficiency reduces positional error arising from linking fluorophores into gel thus paving way for immunolabeled endogenous proteins true molecular resolution.

Language: Английский

Citations

145
The cell biologist's guide to super-resolution microscopy DOI Open Access
Guillaume Jacquemet, Alexandre F. Carisey, Hellyeh Hamidi

et al.

Journal of Cell Science, Journal Year: 2020, Volume and Issue: 133(11)

Published: June 1, 2020

ABSTRACT Fluorescence microscopy has become a ubiquitous method to observe the location of specific molecular components within cells. However, resolution light is limited by laws diffraction few hundred nanometers, blurring most cellular details. Over last two decades, several techniques – grouped under ‘super-resolution microscopy’ moniker have been designed bypass this limitation, revealing organization down nanoscale. The number and variety these steadily increased, point that it difficult for cell biologists seasoned microscopists alike identify technique best suited their needs. Available include image processing strategies generate super-resolved images, optical imaging schemes overcome limit sample manipulations expand size biological sample. In Cell Science at Glance article accompanying poster, we provide key pointers help users navigate through various super-resolution methods briefly summarizing principles behind each technique, highlighting both critical strengths weaknesses, as well providing example images.

Language: Английский

Citations

141