Recent Advances in Stimuli‐Responsive Shape‐Morphing Hydrogels

Advanced Functional Materials, Journal Year: 2022, Volume and Issue: 32(39)

Published: July 14, 2022

Abstract Inspired by shape‐morphing organisms in nature, researchers have developed various hydrogels with stimuli‐responsive swelling, shrinking, bending, folding, origami, rolling, twisting, or locomotion. These smart are usually created patterning 4D printing. The shape morphing of allows the fabrication helixing, and rolling microstructures, all which hard to reproduce directly ordinary techniques. More importantly, under external stimuli (e.g., solvent, humidity, temperature, light, pH, electric/magnetic fields), many exhibit recoverable thus find promising applications grippers, sensors, valves, soft robotics, etc. Since determines functions a great number cases, herein, recent advances summarized, their types, mechanisms, methods, modes, extensive covered. conclusion perspectives also presented guide design functional hydrogels.

Language: Английский

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LAG3 associates with TCR–CD3 complexes and suppresses signaling by driving co-receptor–Lck dissociation

Nature Immunology, Journal Year: 2022, Volume and Issue: 23(5), P. 757 - 767

Published: April 18, 2022

Language: Английский

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Technological advances in super-resolution microscopy to study cellular processes

Molecular Cell, Journal Year: 2022, Volume and Issue: 82(2), P. 315 - 332

Published: Jan. 1, 2022

Language: Английский

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Magnify is a universal molecular anchoring strategy for expansion microscopy

Nature Biotechnology, Journal Year: 2023, Volume and Issue: 41(6), P. 858 - 869

Published: Jan. 2, 2023

Abstract Expansion microscopy enables nanoimaging with conventional microscopes by physically and isotropically magnifying preserved biological specimens embedded in a crosslinked water-swellable hydrogel. Current expansion protocols require prior treatment reactive anchoring chemicals to link specific labels biomolecule classes the gel. We describe strategy called Magnify, which uses mechanically sturdy gel that retains nucleic acids, proteins lipids without need for separate step. Magnify expands up 11 times facilitates imaging of cells tissues effectively around 25-nm resolution using diffraction-limited objective lens about 280 nm on optical or 15 effective if combined super-resolution fluctuation imaging. demonstrate broad range specimens, providing insight into nanoscopic subcellular structures, including synaptic from mouse brain, podocyte foot processes formalin-fixed paraffin-embedded human kidney defects cilia basal bodies drug-treated lung organoids.

Language: Английский

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Spatially resolved transcriptomics: a comprehensive review of their technological advances, applications, and challenges

Journal of genetics and genomics/Journal of Genetics and Genomics, Journal Year: 2023, Volume and Issue: 50(9), P. 625 - 640

Published: March 27, 2023

The ability to explore life kingdoms is largely driven by innovations and breakthroughs in technology, from the invention of microscope 350 years ago recent emergence single-cell sequencing, which scientific community has been able visualize at an unprecedented resolution. Most recently, Spatially Resolved Transcriptomics (SRT) technologies have filled gap probing spatial or even three-dimensional organization molecular foundation behind mysteries life, including origin different cellular populations developed totipotent cells human diseases. In this review, we introduce progress challenges on SRT perspectives bioinformatic tools, as well representative applications. With currently fast-moving promising results early adopted research projects, can foresee bright future such new tools understanding most profound analytical level.

Language: Английский

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Xanthene, cyanine, oxazine and BODIPY: the four pillars of the fluorophore empire for super-resolution bioimaging

Chemical Society Reviews, Journal Year: 2023, Volume and Issue: 52(20), P. 7197 - 7261

Published: Jan. 1, 2023

In the realm of biological research, invention super-resolution microscopy (SRM) has enabled visualization ultrafine sub-cellular structures and their functions in live cells at nano-scale level, beyond diffraction limit, which opened up a new window for advanced biomedical studies to unravel complex unknown details physiological disorders level with unprecedented resolution clarity. However, most SRM techniques are highly reliant on personalized special photophysical features fluorophores. recent times, there been an surge development robust fluorophore systems various imaging techniques. To date, xanthene, cyanine, oxazine BODIPY cores have authoritatively utilized as basic units small-molecule-based organic fluorescent probe designing strategies owing excellent characteristics easy synthetic acquiescence. Since future next-generation will be decided by availability probes these four building blocks play important role progressive design, is urgent need review advancements fluorophores different methods based dye cores. This article not only includes comprehensive discussion about developments emphasis effective integration into cell bio-imaging applications but also critically evaluates background each highlight merits demerits towards developing newer SRM.

Language: Английский

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Stimuli-Responsive Hydrogels: The Dynamic Smart Biomaterials of Tomorrow

Macromolecules, Journal Year: 2023, Volume and Issue: 56(21), P. 8377 - 8392

Published: Oct. 18, 2023

In the past decade, stimuli-responsive hydrogels are increasingly studied as biomaterials for tissue engineering and regenerative medicine purposes. Smart can not only replicate physicochemical properties of extracellular matrix but also mimic dynamic processes that crucial regulation cell behavior. Dynamic changes be influenced by hydrogel itself (isotropic vs anisotropic) or guided applying localized triggers. The resulting swelling–shrinking, shape-morphing, well patterns have been shown to influence function in a spatiotemporally controlled manner. Furthermore, use bioinks 4D bioprinting is very promising they allow biofabrication complex microstructures. This perspective discusses recent cutting-edge advances current challenges field smart engineering. Additionally, emerging trends potential future directions addressed.

Language: Английский

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Light-sheets and smart microscopy, an exciting future is dawning

Communications Biology, Journal Year: 2023, Volume and Issue: 6(1)

Published: May 9, 2023

Abstract Light-sheet fluorescence microscopy has transformed our ability to visualize and quantitatively measure biological processes rapidly over long time periods. In this review, we discuss current future developments in light-sheet that expect further expand its capabilities. This includes smart adaptive imaging schemes overcome traditional trade-offs, i.e., spatiotemporal resolution, field of view sample health. microscopy, a microscope will autonomously decide where, when, what how image. We assess image restoration techniques provide avenues these tradeoffs “open top” microscopes may enable multi-modal with high throughput. As such, predict fulfill an important role biomedical clinical the future.

Language: Английский

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Roadmap on Label‐Free Super‐Resolution Imaging

Laser & Photonics Review, Journal Year: 2023, Volume and Issue: 17(12)

Published: Oct. 30, 2023

Abstract Label‐free super‐resolution (LFSR) imaging relies on light‐scattering processes in nanoscale objects without a need for fluorescent (FL) staining required super‐resolved FL microscopy. The objectives of this Roadmap are to present comprehensive vision the developments, state‐of‐the‐art field, and discuss resolution boundaries hurdles that be overcome break classical diffraction limit label‐free imaging. scope spans from advanced interference detection techniques, where diffraction‐limited lateral is combined with unsurpassed axial temporal resolution, techniques true capability based understanding as an information science problem, using novel structured illumination, near‐field scanning, nonlinear optics approaches, designing superlenses nanoplasmonics, metamaterials, transformation optics, microsphere‐assisted approaches. To end, brings under same umbrella researchers physics biomedical communities which such studies have often been developing separately. ultimate intent paper create current future developments LFSR its physical mechanisms great opening series articles field.

Language: Английский

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iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Nov. 30, 2023

Expansion microscopy (ExM) is a highly effective technique for super-resolution fluorescence that enables imaging of biological samples beyond the diffraction limit with conventional microscopes. Despite development several enhanced protocols, ExM has not yet demonstrated ability to achieve precision nanoscopy techniques such as Single Molecule Localization Microscopy (SMLM). Here, address this limitation, we have developed an iterative ultrastructure expansion (iU-ExM) approach achieves SMLM-level resolution. With iU-ExM, it now possible visualize molecular architecture gold-standard samples, eight-fold symmetry nuclear pores or organization conoid in Apicomplexa. its wide-ranging applications, from isolated organelles cells and tissue, iU-ExM opens new avenues scientists studying structures functions.

Language: Английский

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