Multimodal Phasor Approach to Study Breast Cancer Cell Invasion in a 3D Spheroid Model DOI Creative Commons
Giulia Tedeschi, Francesco Palomba, Lorenzo Scipioni

et al.

Chemical & Biomedical Imaging, Journal Year: 2025, Volume and Issue: unknown

Published: May 15, 2025

Language: Английский

A dual-targeted near-infrared fluorescence lifetime probe for detecting viscosity heterogeneity in arthritic mice DOI

Luolin Wang,

Xing Liang, Hanwen Chi

et al.

Chinese Chemical Letters, Journal Year: 2025, Volume and Issue: unknown, P. 110962 - 110962

Published: Feb. 1, 2025

Language: Английский

Citations

0
Fluorine–Nitrogen Codoped Carbon Dots for Visualization Imaging of Nucleic Acids via Two-Photon Fluorescence Lifetime Microscopy DOI

Yong Guo,

Zhenlong Huang, Luwei Wang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

Fluorescence imaging is a key tool for visualizing the morphology and dynamics of nucleic acids (DNA RNA) in living cells to understand their role regulating growth, development, reproduction organisms. However, effective probes capable simultaneously targeting both DNA RNA, as well tools analyzing distribution relative ratios organisms, are currently lacking. Therefore, fluorine-nitrogen codoped carbon dots with two-photon absorption (F-NCDs) were synthesized by hydrothermal method exhibited stable fluorescence, good biocompatibility, fluorescence lifetime sensitive RNA). The as-prepared F-NCDs act probe quantify distinguish RNA nucleus via multicolor microscopy (TP-FLIM). was particularly tracking changes DNA/RNA plant cell nuclei (onion root tips) during different division stages distinguishing animal tissues (zebrafish). development provides insights into preparation offers an visualization TP-FLIM dynamically study function genetic material various life activities.

Language: Английский

Citations

0
High‐Speed Hyperspectral Imaging for Near Infrared Fluorescence and Environmental Monitoring DOI Creative Commons
Jan Stegemann,

Franziska Gröniger,

Krisztian Neutsch

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Hyperspectral imaging captures both spectral and spatial information from a sample but is intrinsically slow. The near infrared (NIR, > 800 nm) advantageous for applications because it falls into the tissue transparency window also contains vibrational overtone combination modes useful molecular fingerprinting. Here, fast hyperspectral NIR demonstrated using phasor transformation (HyperNIR). A liquid crystal variable retarder (LCVR) used tunable, wavelength-dependent sine- cosine-filtering that transforms optical signals 2D (phasor) space. Spectral thus obtained with just three images. LCVR can be adjusted to cover range 900 1600 nm in windows tunable 50 700 nm, which enables distinguishing fluorophores emission peaks less than 5 apart. Furthermore, label-free reflectance identify plastic polymers monitor vivo plant health. approach uses full camera resolution reaches frame rates of 0.2 s-1, limited only by switching rate LCVR. HyperNIR facilitates straightforward biomedicine environmental monitoring.

Language: Английский

Citations

0
Electrochemical fluorescence modulation enables simultaneous multicolour imaging DOI Creative Commons
Ying Yang, Yuanqing Ma, Alexander Macmillan

et al.

Nature Photonics, Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

Language: Английский

Citations

0
Multimodal Phasor Approach to Study Breast Cancer Cell Invasion in a 3D Spheroid Model DOI Creative Commons
Giulia Tedeschi, Francesco Palomba, Lorenzo Scipioni

et al.

Chemical & Biomedical Imaging, Journal Year: 2025, Volume and Issue: unknown

Published: May 15, 2025

Language: Английский

Citations

0