Linking brain activity across scales with simultaneous opto- and electrophysiology DOI Creative Commons
Chris Lewis, Adrian Hoffmann, Fritjof Helmchen

et al.

Neurophotonics, Journal Year: 2023, Volume and Issue: 11(03)

Published: Sept. 1, 2023

The brain enables adaptive behavior via the dynamic coordination of diverse neuronal signals across spatial and temporal scales: from fast action potential patterns in microcircuits to slower distributed activity brain-wide networks. Understanding principles multiscale dynamics requires simultaneous monitoring multiple, network nodes. Combining optical electrical recordings is promising for collecting data multiple scales can reveal aspects coordinated invisible standard, single-modality approaches. We review recent progress combining opto- electrophysiology, focusing on mouse studies that shed new light function single neurons by embedding their context patterns. Optical readouts be tailored desired tackle specific questions. For example, cells or local populations recorded with multi-electrode arrays related simultaneously acquired report specified subpopulations neurons, non-neuronal cells, neuromodulatory pathways. Conversely, two-photon imaging used densely monitor circuits while sampling distant areas at same time. refinement combined approaches will continue previously inaccessible under-appreciated activity.

Language: Английский

Miniscope-LFOV: A large-field-of-view, single-cell-resolution, miniature microscope for wired and wire-free imaging of neural dynamics in freely behaving animals DOI Creative Commons
Changliang Guo, Garrett J. Blair, Megha Sehgal

et al.

Science Advances, Journal Year: 2023, Volume and Issue: 9(16)

Published: April 21, 2023

Imaging large-population, single-cell fluorescent dynamics in freely behaving animals larger than mice remains a key endeavor of neuroscience. We present large-field-of-view open-source miniature microscope (MiniLFOV) designed for large-scale (3.6 mm × 2.7 mm), cellular resolution neural imaging rats. It has an electrically adjustable working distance up to 3.5 ± 100 μm, incorporates absolute head orientation sensor, and weighs only 13.9 g. The MiniLFOV is capable both deep brain cortical been validated rats by simultaneously >1000 GCaMP7s-expressing neurons the hippocampal CA1 layer head-fixed ~2000 dorsal cortex through cranial window. also supports optional wire-free operation using novel, data acquisition expansion board. expect that this new implementation UCLA Miniscope platform will enable researchers address novel hypotheses concerning function animals.

Language: Английский

Citations

72
Multiphoton fluorescence microscopy for in vivo imaging DOI
Chris Xu, Maiken Nedergaard, Deborah J. Fowell

et al.

Cell, Journal Year: 2024, Volume and Issue: 187(17), P. 4458 - 4487

Published: Aug. 1, 2024

Language: Английский

Citations

34
Imagining the future of optical microscopy: everything, everywhere, all at once DOI Creative Commons
Harikrushnan Balasubramanian, Chad M. Hobson, Teng‐Leong Chew

et al.

Communications Biology, Journal Year: 2023, Volume and Issue: 6(1)

Published: Oct. 28, 2023

The optical microscope has revolutionized biology since at least the 17

Language: Английский

Citations

37
Three-photon excited fluorescence imaging in neuroscience: From principles to applications DOI Creative Commons

Yujie Xiao,

Peng Deng,

Yaoguang Zhao

et al.

Frontiers in Neuroscience, Journal Year: 2023, Volume and Issue: 17

Published: Feb. 20, 2023

The development of three-photon microscopy (3PM) has greatly expanded the capability imaging deep within biological tissues, enabling neuroscientists to visualize structure and activity neuronal populations with greater depth than two-photon imaging. In this review, we outline history physical principles 3PM technology. We cover current techniques for improving performance 3PM. Furthermore, summarize applications various brain regions species. Finally, discuss future neuroscience.

Language: Английский

Citations

18
Engineering brain-on-a-chip platforms DOI
Bram Servais, Negar Mahmoudi, Vini Gautam

et al.

Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: 2(8), P. 691 - 709

Published: June 5, 2024

Language: Английский

Citations

9
A unified open-source platform for multimodal neural recording and perturbation during naturalistic behavior DOI Creative Commons
Jonathan P. Newman, Jie Zhang, Aarón Cuevas-López

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 1, 2023

Behavioral neuroscience faces two conflicting demands: long-duration recordings from large neural populations and unimpeded animal behavior. To meet this challenge, we developed ONIX, an open-source data acquisition system with high throughput (2GB/sec) low closed-loop latencies (<1ms) that uses a novel 0.3 mm thin tether to minimize behavioral impact. Head position rotation are tracked in 3D used drive active commutation without torque measurements. ONIX can acquire combinations of passive electrodes, Neuropixels probes, head-mounted microscopes, cameras, 3D-trackers, other sources. We perform uninterrupted, long (~7 hours) mice as they traversed complex 3-dimensional terrain. allowed exploration similar mobility non-implanted animals, contrast conventional tethered systems which restricted movement. By combining full mobility, our technology will enable new progress on questions require high-quality during ethologically grounded behaviors.

Language: Английский

Citations

14
Deep-brain optical recording of neural dynamics during behavior DOI Creative Commons
Zhe Zhou, Adam Gordon-Fennell, Sean C. Piantadosi

et al.

Neuron, Journal Year: 2023, Volume and Issue: 111(23), P. 3716 - 3738

Published: Oct. 6, 2023

Language: Английский

Citations

14
Cortical Integration of Vestibular and Visual Cues for Navigation, Visual Processing, and Perception DOI Creative Commons

Sepiedeh Keshavarzi,

Mateo Vélez‐Fort, Troy W. Margrie

et al.

Annual Review of Neuroscience, Journal Year: 2023, Volume and Issue: 46(1), P. 301 - 320

Published: July 10, 2023

Despite increasing evidence of its involvement in several key functions the cerebral cortex, vestibular sense rarely enters our consciousness. Indeed, extent to which these internal signals are incorporated within cortical sensory representation and how they might be relied upon for sensory-driven decision-making, during, example, spatial navigation, is yet understood. Recent novel experimental approaches rodents have probed both physiological behavioral significance indicate that their widespread integration with vision improves perceptual accuracy self-motion orientation. Here, we summarize recent findings a focus on circuits involved visual perception navigation highlight major remaining knowledge gaps. We suggest vestibulo-visual reflects process constant updating regarding status self-motion, access such information by cortex used predictions may implemented rapid, navigation-related decision-making.

Language: Английский

Citations

13
Double spiral resonant MEMS scanning for ultra-high-speed miniaturized optical microscopy DOI Creative Commons

Linyang Li,

Liang Xiao, Wei Qin

et al.

Optica, Journal Year: 2023, Volume and Issue: 10(9), P. 1195 - 1195

Published: Aug. 7, 2023

Micro–electro–mechanical systems (MEMS)-based optical scanners play a vital role in the development of miniaturized imaging modalities. However, there is longstanding challenge to balance temporal resolution, field view (FOV), and systematic fidelity. Here, we propose double spiral scanning mechanism enable high-frequency resonant MEMS without sacrificing quality, offer versatile interface for applications different scenarios. This arrangement, demonstrated by photoacoustic endoscopy, shows that rate FOV can be improved more than 60 two times, respectively. The proposed method general address limitations MEMS-based microscopies adapted various modalities, such as intraoperative image-guided surgery, wearable devices.

Language: Английский

Citations

13
Three‐Photon Infrared Stimulation of Endogenous Neuroreceptors in Vivo DOI Creative Commons
Rosalba Sortino, Marina Cunquero, Gustavo Castro‐Olvera

et al.

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 62(51)

Published: Oct. 12, 2023

Abstract To interrogate neural circuits and crack their codes, in vivo brain activity imaging must be combined with spatiotemporally precise stimulation three dimensions using genetic or pharmacological specificity. This challenge requires deep penetration focusing as provided by infrared light multiphoton excitation, has promoted two‐photon photopharmacology optogenetics. However, three‐photon remains to demonstrated. We report the regulation of neuronal zebrafish larvae excitation a photoswitchable muscarinic agonist at 50 pM, billion‐fold lower concentration than used for uncaging, mid‐infrared 1560 nm, longest reported photoswitch wavelength. Robust, physiologically relevant photoresponses allow modulating wild‐type animals spatiotemporal precision. Computational calculations predict that azobenzene‐based ligands have high absorption cross‐section can directly pulsed light. The expansion pharmacology will deeply impact basic neurobiology neuromodulation phototherapies.

Language: Английский

Citations

13