Functional architecture of intracellular oscillations in hippocampal dendrites DOI Creative Commons
Zhenrui Liao, Kevin C. Gonzalez,

Deborah M. Li

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 26, 2024

Fast electrical signaling in dendrites is central to neural computations that support adaptive behaviors. Conventional techniques lack temporal and spatial resolution the ability track underlying membrane potential dynamics present across complex three-dimensional dendritic arbor vivo. Here, we perform fast two-photon imaging of somatic single pyramidal cells CA1 region mouse hippocampus during awake behavior. We study subthreshold suprathreshold events throughout vivo by combining voltage with simultaneous local field recording, post hoc morphological reconstruction, a navigation task. systematically quantify modulation event rates locomotion distinct regions, report an advancing gradient theta phase along basal-tuft axis, describe predominant hyperpolarization sharp-wave ripples. Finally, find tuning representations dynamically reorganizes following place formation. Our data reveal how organization maps onto anatomy tree behavior, oscillatory network, functional cell states. Neurons receive their input three dimensions via dendrites, but activity organized unknown. authors work out rules govern this 3D structure different brain

Language: Английский

Closed-loop experiments and brain machine interfaces with multiphoton microscopy DOI Creative Commons
Riichiro Hira

Neurophotonics, Journal Year: 2024, Volume and Issue: 11(03)

Published: Feb. 19, 2024

In the field of neuroscience, importance constructing closed-loop experimental systems has increased in conjunction with technological advances measuring and controlling neural activity live animals. We provide an overview recent field, focusing on where multiphoton microscopy—the only method capable recording targeted population neurons at a single-cell resolution vivo—works through real-time feedback. Specifically, we present some examples brain machine interfaces (BMIs) using vivo two-photon calcium imaging discuss applications optogenetic stimulation adaptive optics to BMIs. also consider conditions for realizing future optical BMIs synaptic level, their possible roles understanding computational principles brain.

Language: Английский

Citations

2
共聚焦激光扫描检眼镜研究进展与应用（特邀） DOI Open Access

叶夏笛 Ye Xiadi,

黄江杰 Huang Jiangjie,

孔文 Kong Wen

et al.

Laser & Optoelectronics Progress, Journal Year: 2024, Volume and Issue: 61(10), P. 1000003 - 1000003

Published: Jan. 1, 2024

眼底成像对眼科学研究、眼底疾病诊断和治疗都有着重要的意义,而共聚焦激光扫描检眼镜由于具有优越的成像质量和泛用性,并具有独特的轴向分辨能力,在眼底成像领域中已经占据主导地位。主要综述了共聚焦激光扫描检眼镜检查法在超广角眼底成像方面和高分辨率眼底成像方面的实现原理和技术发展,分析了目前仍面临的挑战,并基于现存的挑战对其未来发展进行展望。您的浏览器不支持 audio 元素。AI语音播报

Citations

2
Pixel-wise programmability enables dynamic high-SNR cameras for high-speed microscopy DOI Creative Commons
Jie Zhang, Jonathan P. Newman, Zeguan Wang

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: May 27, 2024

High-speed wide-field fluorescence microscopy has the potential to capture biological processes with exceptional spatiotemporal resolution. However, conventional cameras suffer from low signal-to-noise ratio at high frame rates, limiting their ability detect faint fluorescent events. Here, we introduce an image sensor where each pixel individually programmable sampling speed and phase, so that pixels can be arranged simultaneously sample a ratio. In high-speed voltage imaging experiments, our significantly increases output compared low-noise scientific CMOS camera (~2-3 folds). This gain enables detection of weak neuronal action potentials subthreshold activities missed by standard cameras. Our flexible exposure configurations offers versatile strategies improve signal quality in various experimental conditions.

Language: Английский

Citations

2
High-speed two-photon microscopy with adaptive line-excitation DOI Creative Commons
Yunyang Li, Shu Guo,

Ben Mattison

et al.

Optica, Journal Year: 2024, Volume and Issue: 11(8), P. 1138 - 1138

Published: July 1, 2024

We present a two-photon fluorescence microscope designed for high-speed imaging of neural activity at cellular resolution. Our uses an adaptive sampling scheme with line illumination. Instead building images pixel by via scanning diffraction-limited spot across the sample, our only illuminates regions interest (i.e., neuronal cell bodies) and samples large area them in single measurement. Such significantly increases speed reduces overall laser power on brain tissue. Using this approach, we performed mouse cortex

Language: Английский

Citations

2
Functional architecture of intracellular oscillations in hippocampal dendrites DOI Creative Commons
Zhenrui Liao, Kevin C. Gonzalez,

Deborah M. Li

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 26, 2024

Fast electrical signaling in dendrites is central to neural computations that support adaptive behaviors. Conventional techniques lack temporal and spatial resolution the ability track underlying membrane potential dynamics present across complex three-dimensional dendritic arbor vivo. Here, we perform fast two-photon imaging of somatic single pyramidal cells CA1 region mouse hippocampus during awake behavior. We study subthreshold suprathreshold events throughout vivo by combining voltage with simultaneous local field recording, post hoc morphological reconstruction, a navigation task. systematically quantify modulation event rates locomotion distinct regions, report an advancing gradient theta phase along basal-tuft axis, describe predominant hyperpolarization sharp-wave ripples. Finally, find tuning representations dynamically reorganizes following place formation. Our data reveal how organization maps onto anatomy tree behavior, oscillatory network, functional cell states. Neurons receive their input three dimensions via dendrites, but activity organized unknown. authors work out rules govern this 3D structure different brain

Language: Английский

Citations

2