To Acquire or Not to Acquire: Evaluating Compressive Sensing for Raman Spectroscopy in Biology DOI Creative Commons
Piyush Raj, Lintong Wu, Jeong Hee Kim

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 20, 2024

Raman spectroscopy has revolutionized the field of chemical biology by providing detailed and compositional information with minimal sample preparation. Despite its advantages, technique suffers from low throughput due to weak effect, necessitating long acquisition times expensive equipment. This limitation is particularly acute in time-sensitive applications like bioprocess monitoring dynamic studies. Compressive sensing offers a promising solution reducing burden on measurement hardware, lowering costs, decreasing times. It allows for collection sparse data, which can be computationally reconstructed later. paper explores practical application compressive spontaneous across various biological samples. We demonstrate benefits scenarios requiring portable rapid acquisition, storage, such as skin hydration prediction cellular studies involving drug molecules. Our findings highlight potential overcome traditional limitations spectroscopy, paving way broader adoption research clinical diagnostics.

Language: Английский

Click-free imaging of carbohydrate trafficking in live cells using an azido photothermal probe DOI Creative Commons
Qing Xia, Harini A. Perera, Rylie Bolarinho

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(34)

Published: Aug. 21, 2024

Real-time tracking of intracellular carbohydrates remains challenging. While click chemistry allows bio-orthogonal tagging with fluorescent probes, the reaction permanently alters target molecule and only a single snapshot. Here, we demonstrate click-free mid-infrared photothermal (MIP) imaging azide-tagged in live cells. Leveraging micromolar detection sensitivity for 6-azido-trehalose (TreAz) 300-nm spatial resolution MIP imaging, trehalose recycling pathway mycobacteria, from cytoplasmic uptake to membrane localization, is directly visualized. A peak shift azide spectrum further uncovers interactions between TreAz protein. mapping unreacted after reveals heterogeneity within bacterium. Broader applications azido probes visualize initial steps Leloir yeasts newly synthesized glycans mammalian cells are demonstrated.

Language: Английский

Citations

5
Artificial intelligence in tumor drug resistance: Mechanisms and treatment prospects DOI Creative Commons
Jianyou Gu, Junfeng Zhang,

Silüe Zeng

et al.

Published: Feb. 1, 2025

Language: Английский

Citations

0
Detection of radiosensitive subpopulations ex-vivo with Raman microspectroscopy DOI Creative Commons
Aidan D. Meade,

Adrian Maguire,

Jane Bryant

et al.

Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 15

Published: Feb. 27, 2025

Although significant advances in understanding the molecular drivers of acquired and inherited radiosensitivity have occurred recent decades, a single analytical method which can detect classify remains elusive. Raman microspectroscopy has demonstrated capabilities objective classification various diseases, more recently detection modelling radiobiological effect. In this study, spectroscopy is presented as potential tool for subpopulations represented by four lymphoblastoid cell lines derived from individuals with ataxia telangiectasia (2 lines), non-Hodgkins lymphoma, Turner's syndrome. These are classified respect to population mixed radiosensitivity, lymphocytes drawn both healthy controls, prostate cancer patients. spectroscopic measurements were made ex-vivo after exposure X-ray doses 0 Gy, 50 mGy 500 mGy, parallel radiation-induced G2 chromosomal scores, all samples. Support vector machine models developed on basis spectral data capable discrimination radiosensitive populations before irradiation, superior when spectra subjected non-linear dimensionality reduction (UMAP) opposed linear (PCA) approach. Models samples irradiated in-vitro dose 0Gy found provide highest level performance discriminating between classes, performances F1 = 0.92 ± 0.06 achieved held-out test set. Overall, study suggests that may intrinsic using liquid biopsies.

Language: Английский

Citations

0
Optical Photothermal Infrared Imaging Using Metabolic Probes in Biological Systems DOI
Sydney O. Shuster, Anna E. Curtis, Caitlin M. Davis

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Infrared spectroscopy is a powerful tool for identifying biomolecules. In biological systems, infrared spectra provide information on structure, reaction mechanisms, and conformational change of However, the promise applying imaging to systems has been hampered by low spatial resolution overwhelming water background arising from aqueous nature in-cell in vivo work. Recently, optical photothermal microscopy (OPTIR) overcome these barriers achieved both spatially spectrally resolved images live cells organisms. Here, we determine most effective modes collection commercial OPTIR microscope work samples. We examine three cell lines (Huh-7, differentiated 3T3-L1, U2OS) organisms (Escherichia coli, tardigrades, zebrafish). Our results suggest that provided multifrequency comparable hyperspectral while reducing times 20-fold. also explore utility IR active probes using global site-specific noncanonical azide containing amino acid proteins. find photoreactive are not compatible with OPTIR. demonstrate buffers water. 13C glucose metabolism monitored fat E. coli highlights same probe may be used different pathways. Further, some drugs (e.g., neratinib) have moieties can imaged findings illustrate versatility and, together, direction future dynamic living

Language: Английский

Citations

0
Illuminating life processes by vibrational probes DOI
Naixin Qian, Zhilun Zhao, Elsy El Khoury

et al.

Nature Methods, Journal Year: 2025, Volume and Issue: 22(5), P. 928 - 944

Published: May 1, 2025

Language: Английский

Citations

0
Advanced vibrational microscopes for life science DOI
Ji‐Xin Cheng, Yuhao Yuan, Hongli Ni

et al.

Nature Methods, Journal Year: 2025, Volume and Issue: 22(5), P. 912 - 927

Published: May 1, 2025

Language: Английский

Citations

0
Click-free imaging of carbohydrate trafficking in live cells using an azido photothermal probe DOI Creative Commons
Qing Xia,

Harini A. Perera,

Rylie Bolarinho

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 12, 2024

Real-time tracking of intracellular carbohydrates remains challenging. While click chemistry allows bio-orthogonal tagging with fluorescent probes, the reaction permanently alters target molecule and only a single snapshot. Here, we demonstrate click-free mid-infrared photothermal (MIP) imaging azide-tagged in live cells. Leveraging micromolar detection sensitivity for 6-azido-trehalose (TreAz) 300-nm spatial resolution MIP imaging, trehalose recycling pathway mycobacteria, from cytoplasmic uptake to membrane localization, is directly visualized. A peak shift azide spectrum further uncovers interactions between TreAz protein. mapping unreacted after reveals heterogeneity within bacterium. Broader applications azido probes visualize initial steps Leloir yeasts newly synthesized glycans mammalian cells are demonstrated.

Language: Английский

Citations

2
光学显微成像助力肿瘤精准医疗 DOI Open Access

杨彬 Yang Bin,

岳蜀华 Yue Shuhua,

王璞 Wang Pu

et al.

Chinese Journal of Lasers, Journal Year: 2024, Volume and Issue: 51(9), P. 0907004 - 0907004

Published: Jan. 1, 2024

精准医疗是当下肿瘤诊疗的核心问题,即针对不同的病人定制治疗方案,以获得更好的治疗效果和预后。因肿瘤生物机制及诊疗方案的复杂性,现行基于基因测序和传统表型功能检测的肿瘤精准医疗临床实践,难以有效制定可覆盖大部分病患和药物选择的治疗方案。光学显微成像技术能够对肿瘤组织和细胞中的基因组、蛋白组和代谢组信息进行定量分析和空间定位,识别特定病患肿瘤分子特征和生化反应异常,从而建立起肿瘤基因组和表型组的认知桥梁。本文介绍了不同光学显微成像技术在肿瘤精准医疗领域的相关研究和应用,包括多重免疫荧光(mIF)、荧光原位杂交(FISH)和相干拉曼散射(CRS)等光学显微成像技术。这些技术可以从肿瘤微环境、分子特征和代谢组学等角度进行深入洞察,在肿瘤精准医疗领域发挥着重要作用。

Citations

1
Infrared Spectroscopy of Live Cells Using High-Aspect-Ratio Metal-on-Dielectric Metasurfaces DOI
Aditya Mahalanabish, Steven H. Huang, Dias Tulegenov

et al.

Nano Letters, Journal Year: 2024, Volume and Issue: 24(37), P. 11607 - 11614

Published: Sept. 9, 2024

Fourier transform infrared (FTIR) spectroscopy is widely used for molecular analysis. However, the materials situated in an aqueous environment, a precondition live biological objects such as cells, transmission-based FTIR prevented by strong water absorption of mid-infrared (MIR) light. Reflection-based cellular assays using internal reflection elements (IREs) high-index prisms or flat plasmonic metasurfaces mitigate these issues but suffer from shallow probing volume localized near plasma membrane. Inspired recent introduction high-aspect-ratio nanostructures novel platform manipulating behavior, we demonstrate that integration with tall dielectric dramatically enhances sensing capabilities spectroscopy. We also ability metal-on-dielectric metasurface to transduce intracellular processes, protein translocation high-curvature membrane regions during cell adhesion, into interpretable spectral signatures reflected

Language: Английский

Citations

1
Mid-infrared chemical imaging of living cells enabled by plasmonic metasurfaces DOI
Steven H. Huang, Po-Ting Shen, Aditya Mahalanabish

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 18, 2024

Abstract Mid-Infrared (MIR) chemical imaging provides rich information of biological samples in a label-free and non-destructive manner. Yet, its adoption to live-cell analysis is limited by the strong attenuation MIR light water, often necessitating cell culture geometries that are incompatible with prolonged viability cells standard high-throughput workflow. Here, we introduce new approach microscopy, where imaged through their localized near-field interaction plasmonic metasurface. Chemical contrast distinct molecular groups provided sub-cellular resolution images proteins, lipids, nucleic acids were collected using an inverted microscope. Time-lapse living demonstrated behaviors, including motility, viability, substrate adhesion, can be monitored over extended periods time low-power light. The presented method for non-perturbative cells, which well-suited integration modern screening technologies label-free, high-content cells.

Language: Английский

Citations

1