iScience, Journal Year: 2025, Volume and Issue: 28(6), P. 112659 - 112659

Published: May 14, 2025

Studying human development presents significant ethical and technical challenges. Yet, the integration of stem cell technology engineering tools has provided unprecedented insights into early lineage specification morphogenetic events that shape development. Pre-gastrulation models, such as blastoids, replicate aspects blastocyst formation implantation, enabling research on embryo-maternal interactions. Gastrulation including 2D micropatterned systems 3D gastruloid constructs, provide valuable differentiation, signaling pathways, tissue organization during germ layer formation. Beyond gastrulation, post-gastrulation somitoids, mimicking somitogenesis axial elongation, offer opportunities for studying segmentation neural tube Together, these enable investigation peri-gastrulation stage mammalian Here, we discuss technologies, substrates, microfluidic systems, synthetic biology tools, in enhancing precision allowing a greater understanding stages

Language: Английский