FEBS Journal,
Journal Year:
2022,
Volume and Issue:
289(21), P. 6463 - 6483
Published: Jan. 24, 2022
Regeneration
of
the
mammalian
adult
skeletal
muscle
is
a
well‐orchestrated
process
regulated
by
multiple
proteins
and
signalling
pathways.
Cytokines
constitute
major
class
regulators
myogenesis.
It
well
established
that
infiltrating
immune
cells
at
site
injury
secrete
cytokines,
which
play
critical
roles
in
myofibre
repair
regeneration
process.
In
past
10–15
years,
itself
has
emerged
as
prolific
producer
cytokines.
Much
attention
field
been
focused
on
endocrine
effects
muscle‐secreted
cytokines
(myokines)
metabolic
regulation.
However,
ample
evidence
suggests
muscle‐derived
also
regulate
myogenic
differentiation
an
autocrine
manner.
this
review,
we
survey
meet
two
criteria:
(a)
expression
cells;
(b)
demonstrating
function.
Dozens
representing
several
classes
make
up
group,
together
they
all
steps
How
such
large
array
coordinate
their
to
form
regulatory
network
fascinating,
pressing
question.
Functional
studies
can
distinguish
source
vivo
are
much
needed
order
facilitate
exploration
full
therapeutic
potential.
Molecular Therapy,
Journal Year:
2023,
Volume and Issue:
31(7), P. 1920 - 1937
Published: March 24, 2023
The
CRISPR-Cas
system
is
commonly
known
for
its
ability
to
cleave
DNA
in
a
programmable
manner,
which
has
democratized
gene
editing
and
facilitated
recent
breakthroughs
therapy.
However,
newer
iterations
of
the
technology
using
nuclease-disabled
Cas
enzymes
have
spurred
variety
different
types
genetic
engineering
platforms
such
as
transcriptional
modulation
CRISPR
activation
(CRISPRa)
interference
(CRISPRi)
systems.
This
review
introduces
creation
these
modulators,
various
methods
delivery
utilized
systems,
technological
developments.
CRISPRa
CRISPRi
also
been
implemented
screens
interrogating
function
discovering
genes
involved
biological
pathways.
We
describe
compelling
examples
how
tools
become
powerful
means
unravel
networks
uncovering
important
information
about
devastating
diseases.
Finally,
we
provide
an
overview
preclinical
studies
used
therapeutically,
discuss
potential
future
directions
novel
modalities.
Nature Communications,
Journal Year:
2023,
Volume and Issue:
14(1)
Published: Feb. 23, 2023
DNA
origami
may
enable
more
versatile
gene
delivery
applications
through
its
ability
to
create
custom
nanoscale
objects
with
specific
targeting,
cell-invading,
and
intracellular
effector
functionalities.
Toward
this
goal
here
we
describe
the
expression
of
genes
folded
in
delivered
mammalian
cells.
Genes
readily
express
from
custom-sequence
single-strand
scaffolds
within
objects,
provided
that
can
denature
cell.
We
demonstrate
enhanced
efficiency
by
including
tuning
multiple
functional
sequences
structures,
virus-inspired
inverted-terminal
repeat-like
(ITR)
hairpin
motifs
upstream
or
flanking
cassette.
gene-encoding
bricks
assemble
into
multimeric
stoichiometrically
controlled
co-delivery
same
Our
work
provides
a
framework
for
exploiting
applications.
Annual Review of Genomics and Human Genetics,
Journal Year:
2018,
Volume and Issue:
19(1), P. 43 - 71
Published: May 31, 2018
The
eukaryotic
epigenome
has
an
instrumental
role
in
determining
and
maintaining
cell
identity
function.
Epigenetic
components
such
as
DNA
methylation,
histone
tail
modifications,
chromatin
accessibility,
architecture
are
tightly
correlated
with
central
cellular
processes,
while
their
dysregulation
manifests
aberrant
gene
expression
disease.
ability
to
specifically
edit
the
holds
promise
of
enhancing
understanding
how
epigenetic
modifications
function
enabling
manipulation
phenotype
for
research
or
therapeutic
purposes.
Genome
engineering
technologies
use
highly
specific
DNA-targeting
tools
precisely
deposit
changes
a
locus-specific
manner,
creating
diverse
editing
platforms.
This
review
summarizes
these
insights
from
recent
studies,
describes
complex
relationship
between
regulation,
highlights
caveats
promises
emerging
field
editing,
including
applications
translational
purposes,
therapy
regenerative
medicine.
eNeuro,
Journal Year:
2019,
Volume and Issue:
6(1), P. ENEURO.0495 - 18.2019
Published: Jan. 1, 2019
CRISPR-based
technology
has
provided
new
avenues
to
interrogate
gene
function,
but
difficulties
in
transgene
expression
post-mitotic
neurons
delayed
incorporation
of
these
tools
the
central
nervous
system
(CNS).
Here,
we
demonstrate
a
highly
efficient,
neuron-optimized
dual
lentiviral
transcriptional
activation
(CRISPRa)
capable
robust,
modular,
and
tunable
induction
multiplexed
regulation
across
several
primary
rodent
neuron
culture
systems.
CRISPRa
targeting
unique
promoters
complex
multi-transcript
brain-derived
neurotrophic
factor
(
International Journal of Molecular Sciences,
Journal Year:
2019,
Volume and Issue:
20(23), P. 6041 - 6041
Published: Nov. 30, 2019
The
gene
editing
tool
CRISPR-Cas
has
become
the
foundation
for
developing
numerous
molecular
systems
used
in
research
and,
increasingly,
medical
practice.
In
particular,
Cas
proteins
devoid
of
nucleolytic
activity
(dead
proteins;
dCas)
can
be
to
deliver
functional
cargo
programmed
sites
genome.
this
review,
we
describe
current
CRISPR
different
dCas-based
approaches
and
summarize
their
most
significant
applications.
We
conclude
with
comments
on
state-of-art
field
future
directions.
