Reviewer #3 (Public Review): behaviorMate: An Intranet of Things Approach for Adaptable Control of Behavioral and Navigation-Based Experiments DOI Open Access

Published: July 11, 2024

Investigators conducting behavioral experiments often need precise control over the timing of delivery stimuli to subjects and collect times subsequent responses. Furthermore, investigators want fine-tuned how various multi-modal cues are presented. behaviorMate takes an "Intranet Things" approach, using a networked system hardware software components for achieving these goals. The outputs file with integrated timestamp-event pairs that can then format process their own analysis pipelines. We present overview electronic GUI application make up as well mechanical designs compatible experimental rigs provide reader ability set system. A wide variety paradigms supported, including goal-oriented learning, random foraging, context switching. demonstrate behaviorMate's utility reliability range use cases from several published studies benchmark tests. Finally, we validation demonstrating different modalities hippocampal place field studies. Both treadmill burlap belt virtual reality running wheel were performed confirm efficacy flexibility approach. Previous solutions rely on proprietary systems may have large upfront costs or frameworks require customized be developed. uses open-source flexible configuration mitigate both concerns. has proven record head-fixed imaging could easily adopted task in situations.

Language: Английский

Subfield-specific interneuron circuits govern the hippocampal response to novelty in male mice DOI Creative Commons
Thomas Hainmueller, Aurore Cazala, Li-Wen Huang

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Jan. 24, 2024

Abstract The hippocampus is the brain’s center for episodic memories. Its subregions, dentate gyrus and CA1-3, are differentially involved in memory encoding recall. Hippocampal principal cells represent features like movement, space, context, but less known about GABAergic interneurons. Here, we performed two-photon calcium imaging of parvalbumin- somatostatin-expressing interneurons CA1-3 male mice exploring virtual environments. Parvalbumin-interneurons increased activity with running-speed reduced it novel Somatostatin-interneurons behaved similar to parvalbumin-expressing cells, their counterparts during rest Congruently, chemogenetic silencing parvalbumin-interneurons had prominent effects familiar contexts, while similarity granule cell representations between Our data indicate unique roles somatostatin-positive that distinct from those may support routing information.

Language: Английский

Citations

9
A hippocampal circuit mechanism to balance memory reactivation during sleep DOI
Lindsay A. Karaba, Heath L. Robinson, Ryan E. Harvey

et al.

Science, Journal Year: 2024, Volume and Issue: 385(6710), P. 738 - 743

Published: Aug. 15, 2024

Memory consolidation involves the synchronous reactivation of hippocampal cells active during recent experience in sleep sharp-wave ripples (SWRs). How this increase firing rates and synchrony after learning is counterbalanced to preserve network stability not understood. We discovered a event generated by an intrahippocampal circuit formed subset CA2 pyramidal cholecystokinin-expressing (CCK+) basket cells, which fire barrage action potentials (“BARR”) non–rapid eye movement sleep. CA1 neurons assemblies that increased their activity were reactivated SWRs but inhibited BARRs. The initial returned baseline through This trend was abolished silencing CCK+ BARRs, resulting higher impaired memory consolidation.

Language: Английский

Citations

8
Cholecystokinin neurotransmission in the central nervous system: Insights into its role in health and disease DOI Creative Commons
Muhammad Asim, Huajie Wang, Abdul Waris

et al.

BioFactors, Journal Year: 2024, Volume and Issue: unknown

Published: May 22, 2024

Abstract Cholecystokinin (CCK) plays a key role in various brain functions, including both health and disease states. Despite the extensive research conducted on CCK, there remain several important questions regarding its specific brain. As result, existing body of literature subject is complex sometimes conflicting. The primary objective this review article to provide comprehensive overview recent advancements understanding central nervous system with emphasis elucidating CCK's mechanisms for neuroplasticity, exploring interactions other neurotransmitters, discussing significant involvement neurological disorders. Studies demonstrate that CCK mediates inhibitory long‐term potentiation (iLTP) excitatory (eLTP) Activation GPR173 receptor could facilitate iLTP, while B (CCKBR) facilitates eLTP. receptors' expression different neurons regulates activity, neurotransmitter release, plasticity, emphasizing modulating function. Furthermore, pivotal emotional states, Alzheimer's disease, addiction, schizophrenia, epileptic conditions. Targeting cell types circuits holds promise as therapeutic strategy alleviating these

Language: Английский

Citations

6
behaviorMate: An Intranet of Things Approach for Adaptable Control of Behavioral and Navigation-Based Experiments DOI Open Access
John C. Bowler, George Zakka, Hyun Choong Yong

et al.

Published: Jan. 20, 2025

Investigators conducting behavioral experiments often need precise control over the timing of delivery stimuli to subjects and collect times subsequent responses. Furthermore, investigators want fine-tuned how various multi-modal cues are presented. behaviorMate takes an “Intranet Things” approach, using a networked system hardware software components for achieving these goals. The outputs file with integrated timestamp-event pairs that can then format process their own analysis pipelines. We present overview electronic GUI application make up as well mechanical designs compatible experimental rigs provide reader ability set system. A wide variety paradigms supported, including goal-oriented learning, random foraging, context switching. demonstrate behaviorMate’s utility reliability range use cases from several published studies benchmark tests. Finally, we validation demonstrating different modalities hippocampal place field studies. Both treadmill burlap belt virtual reality running wheel were performed confirm efficacy flexibility approach. Previous solutions rely on proprietary systems may have large upfront costs or frameworks require customized be developed. uses open-source flexible configuration mitigate both concerns. has proven record head-fixed imaging could easily adopted task in situations.

Language: Английский

Citations

0
Reciprocal interactions between CA1 pyramidal and axo-axonic cells control sharp wave-ripple events DOI Creative Commons
Daniel F. English,

Earl T. Gilbert,

Lianne M.F. Klaver

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

Diverse sources of inhibition serve to modulate circuits and control cell assembly spiking across various timescales. For example, in hippocampus area CA1 the competition between excitation organizes spike timing pyramidal cells (PYR) network events, including sharp wave-ripples (SPW-R). Specific cellular-synaptic SPW-R remain unclear, as there are > 20 types GABAergic interneurons CA1. Axo-axonic (AAC) defined by their synaptic targeting axon initial segment cells, potently controlling output. The impact AAC activity on is controversial, due mainly ambiguity identification. Here we monitored manipulated opto-tagged AACs behaving mice using silicon probe recordings. We found a large variability neurons, varying from enhanced suppressed during SPW-Rs, contrast near-uniform other parvalbumin-expressing interneurons. received convergent monosynaptic inputs local assemblies, which strongly influenced participation SPW-Rs. Optogenetic silencing increased power duration recruiting greater number PYR, suggesting dynamics. hypothesize that lateral reciprocal PYR-AAC interactions thus supports organization assemblies SPW-R.

Language: Английский

Citations

0
An Intranet of Things approach for adaptable control of behavioral and navigation-based experiments DOI Creative Commons
John C. Bowler, George Zakka, Hyun Choong Yong

et al.

eLife, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 26, 2025

Investigators conducting behavioral experiments often need precise control over the timing of delivery stimuli to subjects and collect times subsequent responses. Furthermore, investigators want fine-tuned how various multi-modal cues are presented. behaviorMate takes an ‘Intranet Things’ approach, using a networked system hardware software components for achieving these goals. The outputs file with integrated timestamp–event pairs that can then format process their own analysis pipelines. We present overview electronic GUI application make up as well mechanical designs compatible experimental rigs provide reader ability set system. A wide variety paradigms supported, including goal-oriented learning, random foraging, context switching. demonstrate behaviorMate’s utility reliability range use cases from several published studies benchmark tests. Finally, we validation demonstrating different modalities hippocampal place field studies. Both treadmill burlap belt virtual reality running wheel were performed confirm efficacy flexibility approach. Previous solutions rely on proprietary systems may have large upfront costs or frameworks require customized be developed. uses open-source flexible configuration mitigate both concerns. has proven record head-fixed imaging could easily adopted task in situations.

Language: Английский

Citations

0
Goal-specific hippocampal inhibition gates learning DOI Creative Commons
Nuri Jeong, Xiao Xiang Zheng, Abigail L. Paulson

et al.

Nature, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

Goal-directed navigation in a new environment requires quickly identifying and exploiting important locations. Identifying goal locations depends on neural computations that rapidly represent connect location information to key outcomes such as food1. However, the mechanisms trigger these at behaviourally relevant are not well understood. Here we show parvalbumin (PV)-positive interneurons mouse hippocampal CA3 have causal role food decreases inhibitory activity around goals enable reactivation bind outcomes. PV substantially reduce firing approach while food-deprived mice learn find food. These anticipate reward more prominent correct trials. Sparse optogenetic stimulation prevent goal-related interneuron impaired learning of Disrupting after receipt food, process associates previous know where seek later. results reveal goal-selective goal-predictive learning, representations outcome associations crucial

Language: Английский

Citations

0
Integrating endocannabinoid signaling, CCK interneurons, and hippocampal circuit dynamics in behaving animals DOI Creative Commons
Shreya Malhotra, Florian Donneger, Jordan S. Farrell

et al.

Neuron, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Learning, Fast and Slow: Single- and Many-Shot Learning in the Hippocampus DOI
Zhenrui Liao, Attila Losonczy

Annual Review of Neuroscience, Journal Year: 2024, Volume and Issue: 47(1), P. 187 - 209

Published: April 25, 2024

The hippocampus is critical for memory and spatial navigation. ability to map novel environments, as well more abstract conceptual relationships, fundamental the cognitive flexibility that humans other animals require survive in a dynamic world. In this review, we survey recent advances our understanding of how implemented anatomically functionally by hippocampal circuitry, during both active exploration (online) rest (offline). We discuss advantages limitations spike timing–dependent plasticity recently discovered behavioral timescale synaptic supporting distinct learning modes hippocampus. Finally, suggest complementary roles these types explaining many-shot single-shot rules could work together support maps.

Language: Английский

Citations

3
An inhibitory circuit-based enhancer of DYRK1A function reverses Dyrk1a-associated impairment in social recognition DOI Creative Commons

Yu‐Tzu Shih,

Jason Bondoc Alipio, Amar Sahay

et al.

Neuron, Journal Year: 2023, Volume and Issue: 111(19), P. 3084 - 3101.e5

Published: Oct. 1, 2023

Heterozygous mutations in the dual-specificity tyrosine phosphorylation-regulated kinase 1a (Dyrk1a) gene define a syndromic form of autism spectrum disorder. The synaptic and circuit mechanisms mediating DYRK1A functions social cognition are unclear. Here, we identify experience-sensitive mechanism hippocampal mossy fiber-parvalbumin interneuron (PV IN) synapses by which recruits feedforward inhibition CA3 CA2 to promote recognition. We employ genetic epistasis logic cytoskeletal protein, ABLIM3, as substrate DYRK1A. demonstrate that Ablim3 downregulation dentate granule cells adult heterozygous Dyrk1a mice is sufficient restore PV IN-mediated Acute chemogenetic activation INs CA3/CA2 also rescued Together, these findings illustrate how targeting substrates "enhancers function" harbors potential reverse haploinsufficiency-associated impairments.

Language: Английский

Citations

8