Tachykinin1-expressing neurons in the parasubthalamic nucleus control active avoidance learning DOI Open Access

Ruining Hu,

Nannan Wu, Tong Liu

et al.

Published: Dec. 18, 2024

Active avoidance is a type of instrumental behavior that requires an organism actively to engage in specific actions avoid or escape from potentially aversive stimulus and crucial for the survival well-being organisms. It widely distributed, hard-wired neural circuits spanning multiple brain regions, including amygdala thalamus. However, less known about whether how hypothalamus encodes controls active learning. Here we identify previously unknown role parasubthalamic nucleus (PSTN), located lateral subdivision posterior hypothalamus, encoding control Fiber photometry calcium imaging shows activity tachykinin1-expressing PSTN (PSTN Tac1 ) neurons progressively increases during this Cell-type ablation optogenetic inhibition attenuates learning, whereas activation these cells promotes learning via negative motivational drive. Moreover, mediates differentially through its downstream targets. Together, study identifies as new member networks involved offers us potential implications therapeutic interventions targeting anxiety disorders other conditions involving maladaptive

Language: Английский

Tachykinin-1-expressing parasubthalamic nucleus neurons are necessary for odorant-induced appetite suppression DOI

Zoe E Kaegi,

Matthew E. Carter

Physiology & Behavior, Journal Year: 2025, Volume and Issue: unknown, P. 114836 - 114836

Published: Jan. 1, 2025

Language: Английский

Citations

1
Theoretical analysis of low-power deep synergistic sono-optogenetic excitation of neurons by co-expressing light-sensitive and mechano-sensitive ion-channels DOI Creative Commons
Sukhdev Roy,

Gur Pyari,

Himanshu Bansal

et al.

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: March 6, 2025

The present challenge in neuroscience is to non-invasively exercise low-power and high-fidelity control of neurons situated deep inside the brain. Although, two-photon optogenetic excitation can activate millimeter depth with sub-cellular specificity millisecond temporal resolution, it also cause heating targeted tissue. On other hand, sonogenetics modulate cellular activity expressed mechano-sensitive proteins deeper areas brain less spatial selectivity. We a theoretical analysis synergistic sono-optogenetic method overcome these limitations by co-expressing (MscL-I92L) ion-channel light-sensitive (CoChR/ChroME2s/ChRmine) hippocampal neurons. It shown that presence low-amplitude subthreshold ultrasound pulses, threshold for neural spiking reduces drastically 73% MscL-I92L-CoChR (0.021 mW/µm2), 66% MscL-I92L-ChroME2s (0.029 64% MscL-I92L-ChRmine (0.013 mW/µm2) at 5 Hz. allows up 1.2 cm combination. useful design new experiments multimodal neuroprosthetic devices circuits.

Language: Английский

Citations

1
Multifunctional ultraflexible neural probe for wireless optogenetics and electrophysiology DOI Creative Commons
Suhao Wang, Lixuan Li, Shun Zhang

et al.

Giant, Journal Year: 2024, Volume and Issue: 18, P. 100272 - 100272

Published: April 26, 2024

Electrophysiology and optogenetics are pivotal in neuroscience for probing modulating neural activities, playing a vital role unraveling the complexities of brain functionality. Despite their importance, efficacy existing devices is hampered by insufficient functional integration, pronounced foreign body reactions, physical constraints that impede natural animal behaviors. Here, we develop multifunctional, ultraflexible probe designed simultaneous electrophysiological monitoring optical modulation, along with mechanical properties conducive to flexibility compliance. By integrating wireless signal acquisition stimulation circuit, achieved recording signals mice optogenetic control over locomotor behavior. The multifunctional presented this study holds substantial promise closed-loop brain-machine interfaces deepening our understanding circuit functions. This innovative approach addresses aforementioned limitations comprehensive solution vivo interrogation manipulation, marking significant advancement tools available research.

Language: Английский

Citations

4
Tandospirone prevents anesthetic-induced respiratory depression through 5-HT1A receptor activation in rats DOI Creative Commons

Meng-ran Song,

Mingzhi Huang,

Weijie Tao

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 3, 2025

Respiratory depression is a side effect of anesthetics. Treatment with specific antagonists or respiratory stimulants can reverse caused by anesthetics; however, they also interfere the sedative effects Previous studies have suggested that tandospirone may ameliorate without affecting Therefore, we evaluated whether (0.1–8 mg/kg) ameliorates in rat model under anesthesia. The protein kinase A redistribution method was used to determine activates α2a/2c and µ receptors. (10 µM) on α1β2γ2 α4β2δ GABA receptor current modulation were explored two-electrode voltage clamping. Prophylactic administration reduced anesthetics rats. Tandospirone increased SaO2 rats treated fentanyl (80 µg/kg) midazolam (P < 0.05). ability prevent inhibited 5-hydroxytryptamine (5-HT)1 antagonist WAY100635 (1 Co-administration dexmedetomidine did not affect receptors activation. Overall, ameliorated through 5-HT1A

Language: Английский

Citations

0
Hierarchical behavioral analysis framework as a platform for standardized quantitative identification of behaviors DOI Creative Commons
Jialin Ye, Yang Xu, Kang Huang

et al.

Cell Reports, Journal Year: 2025, Volume and Issue: 44(2), P. 115239 - 115239

Published: Feb. 1, 2025

Language: Английский

Citations

0
Defense or death? A review of the neural mechanisms underlying sensory modality-triggered innate defensive behaviors DOI

Huating Gu,

Fan Zhao, Zhihui Liu

et al.

Current Opinion in Neurobiology, Journal Year: 2025, Volume and Issue: 92, P. 102977 - 102977

Published: Feb. 27, 2025

Language: Английский

Citations

0
Methods for Assessing Neurodevelopmental Disorders in Mice: A Critical Review of Behavioral Tests and Methodological Considerations Searching to Improve Reliability DOI Creative Commons
Boniface Echefu, Maria Becker, Dan J. Stein

et al.

NeuroSci, Journal Year: 2025, Volume and Issue: 6(2), P. 27 - 27

Published: March 27, 2025

Many neurobehavioral tests are used for the assessment of human-like behaviors in animals. Most them were developed rodents and animal models that mimic human neurodevelopmental neuropsychiatric disorders (NDDs). We have described assessing social behavior, interaction, communication; restricted repetitive behaviors; cognitive impairment, sensory stimuli, anxiety like motor coordination deviations. These to demonstrate autistic-like behavior as well other NDDs. possible general pitfalls performance such studies, probable individual errors each group specific behavior. The mentioned may induce crucial interpretation results, minimizing reliability defined NDD. It is imperative minimize these use sufficient reliable can many traits disorder, grade severity deviations tested NDD by using a scoring system. Due gender differences clinical presentations NDD, it important carry out studies on males females.

Language: Английский

Citations

0
The parasubthalamic nucleus DOI
Matthew E. Carter

Current Biology, Journal Year: 2025, Volume and Issue: 35(10), P. R365 - R367

Published: May 1, 2025

Language: Английский

Citations

0
Anterior cingulate cortex parvalbumin and somatostatin interneurons shape social behavior in male mice DOI Creative Commons
Chuchu Qi, Wenqi Sima, Honghui Mao

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: May 4, 2025

The anterior cingulate cortex (ACC) is essential for social behavior, and its dysfunction implicated in interaction deficits autism. Pyramidal neuron activity the ACC modulated by parvalbumin (PV) somatostatin (SST) interneurons, though their specific roles interactions remain unclear. Here, we demonstrate that PV SST interneurons differentially contribute to regulation of interactions. In a Shank3-deficient autistic model, expression Kcnh7, risk gene autism, reduced both interneurons. Knocking out Kcnh7 either interneuron subtype leads deficits. Furthermore, projections from lateral posterior thalamic nucleus (mediorostral part, LPMR) ventral hippocampus (vHPC) modulate These findings provide new insights into distinct processes contributions autism-related pathophysiology.

Language: Английский

Citations

0
Machine Learning-Enabled Emotion Recognition by Multisource Throat Signals DOI
Junjie Mao, Zhonghui Shen, Jian Wang

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: May 7, 2025

Emotion monitoring plays a crucial role in mental health management. However, traditional methods of emotion recognition predominantly rely on subjective questionnaires or facial expression analyses, which are often inadequate for continuous and highly accurate monitoring. In this study, we propose high-precision, fine-grained system based multisource throat physiological signals. The collects signals through optimized flexible multiporous skin sensors analyzes them using machine learning models capable efficiently capturing complex feature interactions. First, adopt two-step cross-linking strategy to modulate the porous structure sensitive layer enable detection diverse weak throat. By extracting four-dimensional features from input 7025 samples, platform Light Gradient Boosting Machine (LightGBM) captures their nonlinear interactions, ultimately achieving precise classification five emotional states (relaxation, surprise, disgust, fear, neutral) with an accuracy 98.9%. Further validation independent data set reveals average 99.3%, demonstrating system's robustness reliability real-world applications. This work provides viable technological solution real-time monitoring, offering significant potential management related fields.

Language: Английский

Citations

0