The Functional Connectome Mediating Circadian Synchrony in the Suprachiasmatic Nucleus DOI Creative Commons
K. L. Nikhil, Bharat Singhal, Daniel Granados‐Fuentes

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 11, 2024

Abstract Circadian rhythms in mammals arise from the spatiotemporal synchronization of ∼20,000 neuronal clocks Suprachiasmatic Nucleus (SCN). While anatomical, molecular, and genetic approaches have revealed diverse cell types signaling mechanisms, network wiring that enables SCN cells to communicate synchronize remains unclear. To overcome challenges revealing functional connectivity fixed tissue, we developed MITE (Mutual Information & Transfer Entropy), an information theory approach infers directed cell-cell connections with high fidelity. By analyzing 3447 hours continuously recorded clock gene expression 9011 17 mice, found connectome was highly conserved bilaterally across sparse, organized into a dorsomedial ventrolateral module. most were local, discovered long-range ventral both dorsal SCN. Based on their connectivity, can be characterized as circadian signal generators, broadcasters, sinks, or bridges. For example, subset VIP neurons acts hubs generate signals critical daily neural network. Simulations experimentally inferred networks recapitulated stereotypical dorsal-to-ventral wave PER2 ability spontaneously synchronize, emergent dynamics are sculpted by connectivity. We conclude provides powerful method infer connectomes, architecture mediates synchrony space time mammalian Highlights MITE, method, accurately among cells. patterns spatially organize two regions function bridges information. One-third serve drive Key features mediate generation maintenance intercellular waves

Language: Английский

Subsets of extraocular motoneurons produce kinematically distinct saccades during hunting and exploration DOI Creative Commons
Charles K. Dowell, Thomas Hawkins, Isaac H. Bianco

et al.

Current Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

1
A brainstem integrator for self-location memory and positional homeostasis in zebrafish DOI Creative Commons
En Yang, Maarten Zwart, Ben James

et al.

Cell, Journal Year: 2022, Volume and Issue: 185(26), P. 5011 - 5027.e20

Published: Dec. 1, 2022

To track and control self-location, animals integrate their movements through space. Representations of self-location are observed in the mammalian hippocampal formation, but it is unknown if positional representations exist more ancient brain regions, how they arise from integrated self-motion, by what pathways locomotion. Here, a head-fixed, fictive-swimming, virtual-reality preparation, we exposed larval zebrafish to variety involuntary displacements. They tracked these displacements and, many seconds later, moved toward earlier location corrective swimming ("positional homeostasis"). Whole-brain functional imaging revealed network medulla that stores memory induces an error signal inferior olive drive future swimming. Optogenetically manipulating medullary integrator cells evoked displacement-memory behavior. Ablating them, or downstream olivary neurons, abolished displacement corrections. These results reveal multiregional hindbrain circuit vertebrates integrates self-motion locomotor

Language: Английский

Citations

36
Modular architecture confers robustness to damage and facilitates recovery in spiking neural networks modeling in vitro neurons DOI Creative Commons
Takuma Sumi, Akke Mats Houben, Hideaki Yamamoto

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

Abstract Impaired brain function is restored following injury through dynamic processes that involve synaptic plasticity. This restoration supported by the brain’s inherent modular organization, which promotes functional separation and redundancy. However, it remains unclear how structure interacts with plasticity, most notably in form of spike-timing-dependent plasticity (STDP), to define damage response recovery efficiency. In this work, we numerically modeled a neuronal network vitro bearing structure. Consistent observations, silico numerical model effectively captured decline subsequent spontaneous activity injury. We revealed confers robustness injury, minimizes decrease activity, via STDP. Finally, using reservoir computing framework, show information representation improves synchronous activity. Our work provides an experimental-numerical platform for predicting damaged networks may help developing effective models

Language: Английский

Citations

0
Correlative light and electron microscopy reveals the fine circuit structure underlying evidence accumulation in larval zebrafish DOI Creative Commons
Jonathan Boulanger-Weill, F. Kampf, Richard Schalek

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 15, 2025

Accumulating information is a critical component of most circuit computations in the brain across species, yet its precise implementation at synaptic level remains poorly understood. Dissecting such neural circuits vertebrates requires knowledge functional properties and ability to directly correlate dynamics with underlying wiring diagram same animal. Here we combine calcium imaging ultrastructural reconstruction, using visual motion accumulation paradigm larval zebrafish. Using connectomic analyses functionally identified cells computational modeling, show that bilateral inhibition, disinhibition, recurrent connectivity are prominent motifs for sensory within anterior hindbrain. We also demonstrate similar insights about structure-function relationship this can be obtained through complementary methods involving cell-specific morphological labeling via photo-conversion neuronal response types. used our unique ground truth datasets train test novel classifier algorithm, allowing us assign labels neurons from libraries where lacking. The resulting feature-rich library identities connectomes enabled constrain biophysically realistic network model hindbrain reproduce observed make testable predictions future experiments. Our work exemplifies power hypothesis-driven electron microscopy paired recordings gain mechanistic into signal processing provides framework dissecting vertebrates.

Language: Английский

Citations

0
Wiring of a low-dimensional integrator network DOI

Bo Hua Hu,

Rainer W. Friedrich

Nature Neuroscience, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 26, 2024

Language: Английский

Citations

0
The Functional Connectome Mediating Circadian Synchrony in the Suprachiasmatic Nucleus DOI Creative Commons
K. L. Nikhil, Bharat Singhal, Daniel Granados‐Fuentes

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 11, 2024

Abstract Circadian rhythms in mammals arise from the spatiotemporal synchronization of ∼20,000 neuronal clocks Suprachiasmatic Nucleus (SCN). While anatomical, molecular, and genetic approaches have revealed diverse cell types signaling mechanisms, network wiring that enables SCN cells to communicate synchronize remains unclear. To overcome challenges revealing functional connectivity fixed tissue, we developed MITE (Mutual Information & Transfer Entropy), an information theory approach infers directed cell-cell connections with high fidelity. By analyzing 3447 hours continuously recorded clock gene expression 9011 17 mice, found connectome was highly conserved bilaterally across sparse, organized into a dorsomedial ventrolateral module. most were local, discovered long-range ventral both dorsal SCN. Based on their connectivity, can be characterized as circadian signal generators, broadcasters, sinks, or bridges. For example, subset VIP neurons acts hubs generate signals critical daily neural network. Simulations experimentally inferred networks recapitulated stereotypical dorsal-to-ventral wave PER2 ability spontaneously synchronize, emergent dynamics are sculpted by connectivity. We conclude provides powerful method infer connectomes, architecture mediates synchrony space time mammalian Highlights MITE, method, accurately among cells. patterns spatially organize two regions function bridges information. One-third serve drive Key features mediate generation maintenance intercellular waves

Language: Английский

Citations

0