The global phosphorylation landscape of mouse oocytes during meiotic maturation DOI Creative Commons
Hongzheng Sun, Longsen Han, Yueshuai Guo

et al.

The EMBO Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 10, 2024

Abstract Phosphorylation is a key post-translational modification regulating protein function and biological outcomes. However, the phosphorylation dynamics orchestrating mammalian oocyte development remains poorly understood. In present study, we apply high-resolution mass spectrometry-based phosphoproteomics to obtain first global in vivo quantification of mouse phosphorylation. Of more than 8000 phosphosites, 75% significantly oscillate 64% exhibit marked upregulation during meiotic maturation, indicative dominant regulatory role. Moreover, identify numerous novel phosphosites on proteins few highly conserved oocytes from different species. Through functional perturbations, demonstrate that status specific sites participates modulating critical events including metabolism, translation, RNA processing meiosis. Finally, combine inhibitor screening enzyme-substrate network prediction discover previously unexplored kinases phosphatases are essential for maturation. sum, our data define landscape phosphoproteome, enabling in-depth mechanistic insights into developmental control germ cells.

Language: Английский

Epigenetic marks or not? The discovery of novel DNA modifications in eukaryotes DOI

Wei-Ying Meng,

Zixin Wang, Yunfang Zhang

et al.

Journal of Biological Chemistry, Journal Year: 2024, Volume and Issue: 300(4), P. 106791 - 106791

Published: Feb. 23, 2024

Language: Английский

Citations

8
The Mammalian Oocyte: A Central Hub for Cellular Reprogramming and Stemness DOI Creative Commons
Islam M. Saadeldin, Seif Ehab,

Mashan Essa F Alshammari

et al.

Stem Cells and Cloning Advances and Applications, Journal Year: 2025, Volume and Issue: Volume 18, P. 15 - 34

Published: Feb. 1, 2025

The mammalian oocyte is pivotal in reproductive biology, acting as a central hub for cellular reprogramming and stemness. It uniquely contributes half of the zygotic nuclear genome entirety mitochondrial genome, ensuring individual development health. Oocyte-mediated reprogramming, exemplified by transfer, resets somatic cell identity to achieve pluripotency has transformative potential regenerative medicine. This process critical understanding differentiation, improving assisted technologies, advancing cloning stem research. During fertilization, maternal-zygotic transition shifts developmental control from maternal factors activation, establishing totipotency. Oocytes also harbor that guide remodeling, epigenetic modifications, metabolic enabling early embryogenesis. Structures like mitochondria, lipid droplets, cytoplasmic lattices contribute energy production, molecular regulation, organization. Recent insights into components, such ooplasmic nanovesicles endolysosomal vesicular assemblies (ELVAS), highlight their roles maintaining homeostasis, protein synthesis, efficiency. By unraveling mechanisms inherent oocytes, we advance our cloning, therapy, highlighting valuable significance biology

Language: Английский

Citations

1
Distinct dynamics of parental 5-hydroxymethylcytosine during human preimplantation development regulate early lineage gene expression DOI Creative Commons
Dan Liang, Rui Yan, Xin Long

et al.

Nature Cell Biology, Journal Year: 2024, Volume and Issue: 26(9), P. 1458 - 1469

Published: July 30, 2024

The conversion of DNA 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) by TET enzymes represents a significant epigenetic modification, yet its role in early human embryos remains largely unknown. Here we showed that the embryo inherited amount 5hmCs from an oocyte, which unexpectedly underwent de novo hydroxymethylation during growth. Furthermore, generation 5hmC paternal genome after fertilization roughly followed maternal pattern, was linked methylation dynamics and regions sustained methylation. persisted until eight-cell stage exhibited high enrichment at OTX2 binding sites, whereas knockdown compromised expression lineage genes. Specifically, depletion affected activation embryonic genes, further evaluated ectopically expressing mouse Tet3 embryos. These findings revealed distinct unravelled multifaceted functions development.

Language: Английский

Citations

5
Role of epigenetic regulation in diminished ovarian reserve DOI Creative Commons
Wen Chen, Liwei Dong, Chaofeng Wei

et al.

Journal of Assisted Reproduction and Genetics, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 7, 2024

Diminished ovarian reserve (DOR) is characterized by a decrease in the number and quality of oocytes, with its incidence increasing annually. Its pathogenesis remains unclear, making it one most challenging problems field assisted reproduction. Epigenetic modification, molecular mechanism affecting genomic activity expression without altering DNA sequence, has been widely studied reproductive medicine attracted considerable attention regarding DOR. This review comprehensively examines various epigenetic regulatory changes granulosa cells (OGCs) oocytes during methylation plays crucial role regulating cell function, hormone production, oocyte development, maturation, senescence. Histone modifications are involved follicular activation, while non-coding RNAs, such as long noncoding RNAs (lncRNAs) microRNAs (miRNAs), regulate function development. N6-methyladenosine (m6A) associated age-related clocks based on show potential predicting Furthermore, discusses for utilizing mechanisms to better diagnose manage

Language: Английский

Citations

3
Low-complexity regions in fungi display functional groups and are depleted in positively charged amino acids DOI Creative Commons
Kamil Steczkiewicz,

Aleksander Kossakowski,

Stanisław Janik

et al.

NAR Genomics and Bioinformatics, Journal Year: 2025, Volume and Issue: 7(1)

Published: Jan. 7, 2025

Reports on the diversity and occurrence of low-complexity regions (LCR) in Eukaryota are limited. Some studies have provided a more extensive characterization LCR proteins prokaryotes. There is growing body knowledge about plethora biological functions attributable to LCRs. However, it hard determine what extent observed phenomena apply fungi since most fungal LCRs were limited model yeasts. To fill this gap, we performed survey across all tree life branches. We show that abundance with positively correlated proteome size. present protein domains but do not overlap domain regions. associated many duplicated domains. The quantity particular amino acids deviates from background frequency clear over-representation functional groups negative charge. Moreover, discovered each lineage favors distinct expansions. Early diverging lineages differ composition pointing at different evolutionary trajectory group.

Language: Английский

Citations

0
The global phosphorylation landscape of mouse oocytes during meiotic maturation DOI Creative Commons
Hongzheng Sun, Longsen Han, Yueshuai Guo

et al.

The EMBO Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 10, 2024

Abstract Phosphorylation is a key post-translational modification regulating protein function and biological outcomes. However, the phosphorylation dynamics orchestrating mammalian oocyte development remains poorly understood. In present study, we apply high-resolution mass spectrometry-based phosphoproteomics to obtain first global in vivo quantification of mouse phosphorylation. Of more than 8000 phosphosites, 75% significantly oscillate 64% exhibit marked upregulation during meiotic maturation, indicative dominant regulatory role. Moreover, identify numerous novel phosphosites on proteins few highly conserved oocytes from different species. Through functional perturbations, demonstrate that status specific sites participates modulating critical events including metabolism, translation, RNA processing meiosis. Finally, combine inhibitor screening enzyme-substrate network prediction discover previously unexplored kinases phosphatases are essential for maturation. sum, our data define landscape phosphoproteome, enabling in-depth mechanistic insights into developmental control germ cells.

Language: Английский

Citations

1