Protein Science, Journal Year: 2024, Volume and Issue: 33(11)

Published: Oct. 29, 2024

Abstract The inner membrane associated protein of 30 kDa (IM30), a member the endosomal sorting complex required for transport (ESCRT‐III) superfamily, is crucially involved in biogenesis and maintenance thylakoid membranes cyanobacteria chloroplasts. In solution, IM30 assembles into various large oligomeric barrel‐ or tube‐like structures, whereas upon binding it forms large, flat carpet structures. Dynamic localization to changes states are crucial its vivo function. ESCRT‐III proteins known form structures that dynamically assembled from monomeric/smaller proteins, thus these smaller building blocks must be sequentially highly orchestrated manner, still poorly understood process. impact oligomerization on function remains difficult study due high intrinsic tendency homo‐oligomerize. Here, we used molecular dynamics simulations investigate stability individual helices identified unstable regions may provide structural flexibility. Urea‐mediated disassembly barrel was spectroscopically monitored, as well protein's tertiary secondary structure. experimental data were finally compared three‐state model describes oligomer monomer unfolding. this study, stable conserved core discuss advantages having flexible intermediate their putative relevance proteins.

Language: Английский