Reference Gene-Assisted LAMP–LFD for Sensitive and Specific Detection of Soy DNA as a Marker for Allergen Presence in Complex Food Products

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Sensitive, selective screening for allergenic ingredients with internal control is crucial to identify food adulteration and remove allergens from the chain. Here, loop-mediated isothermal amplification combined a lateral flow device (LAMP-LFD) was developed fast easy detection of soy DNA. The integrated quality controls included regular line ensure proper implementation (extraction control) second LAMP-LFD assay cytochrome oxidase gene, which housekeeping gene in plants. LAMP showed limit only 5 pg DNA input per reaction, both pure spiked samples. test subsequently implemented examination 32 real products different compositions declared contents, benchmarked against qPCR. Then, this system digital cube reader, allowing direct interpretation results, facilitating point-of-need applicability.

Language: Английский

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Highly Selective Colorimetric and Smartphone-Based Paper Assay Using Malic Acid–Functionalized Silver Nanoparticles for Thiram Detection

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: May 13, 2025

Language: Английский

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Aptamer&MOF-functionalized multichannel paper chip for point-of-care testing of small extracellular vesicle membrane protein profile

Chinese Chemical Letters, Journal Year: 2025, Volume and Issue: unknown, P. 111333 - 111333

Published: May 1, 2025

Language: Английский

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Multiplexed Centrifugal Microfluidic System for Dynamic Solid-Phase Purification of Polynucleic Acids Direct from Buccal Swabs

Analytical Chemistry, Journal Year: 2021, Volume and Issue: 93(19), P. 7300 - 7309

Published: May 6, 2021

This report describes the development of a centrifugally controlled microfluidic dynamic solid-phase extraction (dSPE) platform to reliably obtain amplification-ready nucleic acids (NAs) directly from buccal swab cuttings. To our knowledge, this work represents first centrifugal microdevice for comprehensive preparation high-purity NAs raw samples. Direct-from-swab cellular lysis was integrated upstream NA extraction, and automatable laser-controlled on-board microvalving strategies provided strict spatiotemporal fluidic control required practical point-of-need use. Solid-phase manipulation during leveraged application bidirectional rotating magnetic field promote thorough interaction with sample (e.g., capture). We illustrate broad utility technology by establishing downstream compatibility extracted three noteworthy assays, namely, polymerase chain reaction (PCR), reverse transcriptase PCR (RT-qPCR), loop-mediated isothermal amplification (LAMP). The PCR-readiness DNA confirmed generating short tandem repeat (STR) profiles following multiplexed amplification. With no changes assay workflow, viral RNA successfully contrived (spiked) SARS-CoV-2 samples, RT-qPCR. Finally, we demonstrate LAMP—a technique well suited genetic analysis due minimal hardware requirements colorimetric readout. describe an automatable, portable acid device that could permit practical, in situ use nontechnical personnel.

Language: Английский

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Automatic quantification of living cells via a non-invasive achromatic colorimetric sensor through machine learning-assisted image analysis using a smartphone

Chemical Engineering Journal, Journal Year: 2022, Volume and Issue: 450, P. 138281 - 138281

Published: July 25, 2022

Language: Английский

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