Regulation of Transmembrane Current through Modulation of Biomimetic Lipid Membrane Composition DOI

Zhiwei Shang,

Jing Zhao, Mengyu Yang

et al.

Faraday Discussions, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 23, 2024

Ion transport through biological channels is influenced not only by the structural properties of themselves but also composition phospholipid membrane, which acts as a scaffold for these nanochannels. Drawing inspiration from how lipid membrane modulates ion currents, seen in activation K

Language: Английский

Single-Molecule Multivalent Interactions Revealed by Plasmon-Enhanced Fluorescence DOI
Kasper R. Okholm, Sjoerd W. Nooteboom, Johan Nygaard Vinther

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(52), P. 35429 - 35442

Published: Dec. 17, 2024

Multivalency as an interaction principle is widely utilized in nature. It enables specific and strong binding by multiple weak interactions through enhanced avidity a core process immune recognition cellular signaling, which also current concept drug design. Here, we use the high signals from plasmon-enhanced fluorescence of nanoparticles to extract kinetics dynamics multivalent on single-molecule level real time. We study mono-, bi-, trivalent using DNA Holliday Junction model construct with programmable valency introduce step-binding for relevant structured macromolecules including experimentally extractable restriction term ω quantify effects conformation, steric effects, rigidity. used this approach explore how length flexibility ligands affect strength, where overall strength decreased spacer length. For systems, increasing additionally activated state, giving insight into design or targeting moieties. By systematically changing receptor density, explored super selectivity HJ at level. find polynomial behavior that clearly shows receptor-density-dependent selective binding. Interestingly, could exploit rapidly decaying near fields plasmon induce dependence signal position dye observe during single events.

Language: Английский

Citations

1
An inverted tetrahedron-mediated DNA walker for sulfadimethoxine detection DOI
Tai Ye, Z. David Luo, Yanting Che

et al.

Microchimica Acta, Journal Year: 2024, Volume and Issue: 191(12)

Published: Nov. 4, 2024

Language: Английский

Citations

0
Regulation of Transmembrane Current through Modulation of Biomimetic Lipid Membrane Composition DOI

Zhiwei Shang,

Jing Zhao, Mengyu Yang

et al.

Faraday Discussions, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 23, 2024

Ion transport through biological channels is influenced not only by the structural properties of themselves but also composition phospholipid membrane, which acts as a scaffold for these nanochannels. Drawing inspiration from how lipid membrane modulates ion currents, seen in activation K

Language: Английский

Citations

0