Abstract
This
review
addresses
the
concept
and
mechanisms
of
bioorthogonal
supramolecular
self-assembly
in
living
organisms.
It
introduces
chemistry,
which
allows
chemical
reactions
to
occur
within
biological
systems
without
interfering
with
natural
processes.
Key
reviewed
include
intracellular
polymerization,
host–guest
interactions
involving
macrocyclic
molecules,
enzyme-mediated
assembly,
peptide
self-assembly.
These
processes
mimic
way
nature
constructs
complex
structures
are
crucial
for
applications
drug
delivery,
bioimaging,
diagnostics,
paving
advancements
personalized
medicine
cancer
therapy.
ACS Nano,
Journal Year:
2024,
Volume and Issue:
18(44), P. 30224 - 30246
Published: Oct. 23, 2024
Each
organelle
referring
to
a
complex
multiorder
architecture
executes
respective
biological
processes
via
its
distinct
spatial
organization
and
internal
microenvironment.
As
the
assembly
of
biomolecules
is
structural
basis
living
cells,
creating
synthetic
nanoassemblies
with
specific
physicochemical
morphological
properties
in
cells
interfere
or
couple
natural
architectures
has
attracted
great
attention
precision
therapeutics
cancers.
In
this
review,
we
give
an
overview
latest
advances
for
precise
regulation,
including
formation
mechanisms,
triggering
strategies,
biomedical
applications
therapeutics.
We
summarize
emerging
material
systems,
polymers,
peptides,
deoxyribonucleic
acids
(DNAs),
their
intermolecular
interactions
intercellular
nanoassemblies,
highlight
design
principles
constructing
precursors
that
assemble
into
targeting
organelles
cellular
environment.
further
showcase
developed
intracellular
mitochondria,
endoplasmic
reticulum,
lysosome,
Golgi
apparatus,
nucleus
describe
underlying
mechanisms
regulation
cancer.
Last,
essential
challenges
field
prospects
future
are
discussed.
This
review
should
facilitate
rational
organelle-targeting
comprehensive
recognition
by
materials
contribute
deep
understanding
application
Journal of the American Chemical Society,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 4, 2025
Intracellular
radical
polymerizations
allow
for
the
direct
bioorthogonal
synthesis
of
various
synthetic
polymers
within
living
cells,
thereby
providing
a
pathway
to
polymer-modified
cells
or
fermentative
production
polymers.
Here,
we
show
that
Escherichia
coli
can
initiate
polymerization
acrylamide,
acrylic,
and
methacrylic
monomers
through
an
atom
transfer
reaction
triggered
by
activity
naturally
occurring
biomolecules
bacterial
cells.
were
confirmed
nuclear
magnetic
resonance
spectroscopy,
gel
permeation
chromatography
extracted
from
fluorescence
labeling
polymer
directly
inside
The
effect
on
cell
behavior
response
was
investigated
microscopy
flow
cytometry
techniques,
as
well
metabolic
membrane
integrity
assays.
resulting
products
are
cell-compatible,
indicated
high
viability
polymerized
In
cellulo
containing
fluorescent
dyes
also
achieved.
These
results
not
only
enhance
our
understanding
untapped
potential
catalysts
but
reveal
intracellular
based
initiators
tool
engineering
biology.
JACS Au,
Journal Year:
2024,
Volume and Issue:
4(11), P. 4162 - 4186
Published: Nov. 1, 2024
A
living
cell
is
an
intricate
machine
that
creates
subregions
to
operate
functions
effectively.
Subcellular
dysfunction
has
been
identified
as
a
potential
druggable
target
for
successful
drug
design
and
therapy.
The
treatments
based
on
intracellular
polymerization,
self-assembly,
or
transformation
offer
various
advantages,
including
enhanced
blood
circulation
of
monomers,
long-term
delivery
pharmacokinetics,
low
resistance,
the
ability
deep
tissues
organelles.
In
this
review,
we
discuss
latest
developments
synthesis
applied
precisely
control
cellular
functions.
First,
applications
endogenous
exogenous
stimuli-triggered
dynamic
morphology
biomolecules
at
subcellular
level.
Second,
highlight
benefits
these
strategies
in
cancer
diagnosis
treatment
modulating
states
metabolism
systems.
Finally,
conclude
recent
progress
field,
future
perspectives,
analyze
challenges
functional
reactions
regulation,
find
opportunities.
Angewandte Chemie International Edition,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Aug. 1, 2024
Abstract
Within
living
organisms,
numerous
nanomachines
are
constantly
involved
in
complex
polymerization
processes,
generating
a
diverse
array
of
biomacromolecules
for
maintaining
biological
activities.
Transporting
artificial
polymerizations
from
lab
settings
into
contexts
has
expanded
opportunities
understanding
and
managing
events,
creating
novel
cellular
compartments,
introducing
new
functionalities.
This
review
summarizes
the
recent
advancements
polymerizations,
including
those
responding
to
external
stimuli,
internal
environmental
factors,
that
polymerize
spontaneously.
More
importantly,
cutting‐edge
biomedical
application
scenarios
polymerization,
notably
safeguarding
cells,
modulating
improving
diagnostic
performance,
facilitating
therapeutic
efficacy
highlighted.
Finally,
this
outlines
key
challenges
technological
obstacles
remain
as
well
offers
insights
potential
directions
advancing
their
practical
applications
clinical
trials.
Angewandte Chemie,
Journal Year:
2024,
Volume and Issue:
136(43)
Published: Aug. 1, 2024
Abstract
Within
living
organisms,
numerous
nanomachines
are
constantly
involved
in
complex
polymerization
processes,
generating
a
diverse
array
of
biomacromolecules
for
maintaining
biological
activities.
Transporting
artificial
polymerizations
from
lab
settings
into
contexts
has
expanded
opportunities
understanding
and
managing
events,
creating
novel
cellular
compartments,
introducing
new
functionalities.
This
review
summarizes
the
recent
advancements
polymerizations,
including
those
responding
to
external
stimuli,
internal
environmental
factors,
that
polymerize
spontaneously.
More
importantly,
cutting‐edge
biomedical
application
scenarios
polymerization,
notably
safeguarding
cells,
modulating
improving
diagnostic
performance,
facilitating
therapeutic
efficacy
highlighted.
Finally,
this
outlines
key
challenges
technological
obstacles
remain
as
well
offers
insights
potential
directions
advancing
their
practical
applications
clinical
trials.
Journal of Materials Chemistry B,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Jan. 1, 2024
Key
intracellular
in
situ
synthesis
processes,
including
the
of
near-infrared
fluorescent
dyes,
oxidative
cross-linking,
polymerization,
and
bioorthogonal
reactions,
as
well
their
biomedical
applications
were
summarized.
